Gene description for RIOK2
Gene name RIO kinase 2
Gene symbol RIOK2
Other names/aliases RIO2
Species Homo sapiens
 Database cross references - RIOK2
ExoCarta ExoCarta_55781
Vesiclepedia VP_55781
Entrez Gene 55781
HGNC 18999
UniProt Q9BVS4  
 RIOK2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RIOK2
Molecular Function
    protein kinase activity GO:0004672 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of mitotic metaphase/anaphase transition GO:0030071 IMP
    maturation of SSU-rRNA GO:0030490 IBA
    maturation of SSU-rRNA GO:0030490 IMP
    ribosomal small subunit biogenesis GO:0042274 IMP
    protein autophosphorylation GO:0046777 IMP
    positive regulation of ribosomal small subunit export from nucleus GO:2000208 IMP
    positive regulation of rRNA processing GO:2000234 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IMP
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    preribosome, small subunit precursor GO:0030688 IBA
    preribosome, small subunit precursor GO:0030688 IDA
 Experiment description of studies that identified RIOK2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RIOK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOB1  
Affinity Capture-MS Homo sapiens
2 AMY2A 279
Affinity Capture-MS Homo sapiens
3 EIF3B 8662
Affinity Capture-MS Homo sapiens
4 RELA 5970
Two-hybrid Homo sapiens
5 H2AFJ 55766
Affinity Capture-MS Homo sapiens
6 VPS16 64601
Affinity Capture-MS Homo sapiens
7 RPL26L1 51121
Affinity Capture-MS Homo sapiens
8 RIT1 6016
Negative Genetic Homo sapiens
9 EIF3A 8661
Affinity Capture-MS Homo sapiens
10 BCAR3 8412
Affinity Capture-MS Homo sapiens
11 Cep120  
Affinity Capture-MS Mus musculus
12 ABT1 29777
Affinity Capture-MS Homo sapiens
13 ZRANB2 9406
Co-fractionation Homo sapiens
14 FBXL6  
Affinity Capture-MS Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 SERF2 10169
Affinity Capture-MS Homo sapiens
17 JADE1  
Affinity Capture-MS Homo sapiens
18 ABCF3 55324
Affinity Capture-MS Homo sapiens
19 EHHADH 1962
Affinity Capture-MS Homo sapiens
20 G3BP1 10146
Affinity Capture-MS Homo sapiens
21 VPS35 55737
Affinity Capture-MS Homo sapiens
22 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
23 TXNL4B  
Affinity Capture-Western Homo sapiens
24 BYSL 705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
25 EPHX1 2052
Co-fractionation Homo sapiens
26 VPS26A 9559
Affinity Capture-MS Homo sapiens
27 C1orf35  
Affinity Capture-MS Homo sapiens
28 CSNK2A1 1457
Biochemical Activity Homo sapiens
29 FOXN2  
Affinity Capture-MS Homo sapiens
30 MINK1 50488
Affinity Capture-MS Homo sapiens
31 MRPS27 23107
Affinity Capture-MS Homo sapiens
32 GHITM 27069
Affinity Capture-MS Homo sapiens
33 BEND3  
Affinity Capture-MS Homo sapiens
34 APP 351
Reconstituted Complex Homo sapiens
35 MYO1C 4641
Affinity Capture-MS Homo sapiens
36 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
37 HP1BP3 50809
Affinity Capture-MS Homo sapiens
38 RPS11 6205
Affinity Capture-MS Homo sapiens
39 AKR7L 246181
Affinity Capture-MS Homo sapiens
40 NUP50 10762
Affinity Capture-MS Homo sapiens
41 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 LBH  
Affinity Capture-MS Homo sapiens
43 TSTA3 7264
Two-hybrid Homo sapiens
44 SPCS1 28972
Affinity Capture-MS Homo sapiens
45 LTV1  
Affinity Capture-MS Homo sapiens
46 CAPN12  
Affinity Capture-MS Homo sapiens
47 HECTD1 25831
Affinity Capture-MS Homo sapiens
48 PARK2  
Affinity Capture-MS Homo sapiens
49 ACACA 31
Affinity Capture-MS Homo sapiens
50 EGFR 1956
Affinity Capture-MS Homo sapiens
51 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
52 SPIN1  
Affinity Capture-MS Homo sapiens
53 RPS6 6194
Affinity Capture-MS Homo sapiens
54 RPSAP58 388524
Affinity Capture-MS Homo sapiens
55 RPL27A 6157
Affinity Capture-MS Homo sapiens
56 PARP1 142
Affinity Capture-MS Homo sapiens
57 STK11 6794
Affinity Capture-MS Homo sapiens
58 NSRP1  
Affinity Capture-MS Homo sapiens
59 ANAPC15  
Affinity Capture-MS Homo sapiens
60 SMAD3 4088
Two-hybrid Homo sapiens
61 KRAS 3845
Negative Genetic Homo sapiens
62 HNRNPU 3192
Affinity Capture-MS Homo sapiens
63 TMEM63A 9725
Affinity Capture-MS Homo sapiens
64 RPS8 6202
Affinity Capture-MS Homo sapiens
65 Rpl35 66489
Affinity Capture-MS Mus musculus
66 CDC20B  
Affinity Capture-MS Homo sapiens
67 BIRC8  
Affinity Capture-MS Homo sapiens
68 TSR1 55720
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
69 SOGA3  
Affinity Capture-MS Homo sapiens
70 EIF3L 51386
Affinity Capture-MS Homo sapiens
71 CAPZB 832
Affinity Capture-MS Homo sapiens
72 FAM207A  
Co-crystal Structure Homo sapiens
73 RPRD1A  
Two-hybrid Homo sapiens
74 SRPK1 6732
Affinity Capture-MS Homo sapiens
75 FBXO5  
Affinity Capture-MS Homo sapiens
76 RPS16 6217
Affinity Capture-MS Homo sapiens
77 EWSR1 2130
Affinity Capture-MS Homo sapiens
78 RPS13 6207
Affinity Capture-MS Homo sapiens
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