Gene description for ODF2
Gene name outer dense fiber of sperm tails 2
Gene symbol ODF2
Other names/aliases CT134
ODF2/1
ODF2/2
ODF84
Species Homo sapiens
 Database cross references - ODF2
ExoCarta ExoCarta_4957
Vesiclepedia VP_4957
Entrez Gene 4957
HGNC 8114
MIM 602015
UniProt Q5BJF6  
 ODF2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ODF2
Molecular Function
    structural molecule activity GO:0005198 TAS
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
Biological Process
    spermatogenesis GO:0007283 IEA
    protein localization GO:0008104 IMP
    centriole-centriole cohesion GO:0010457 IGI
    cell differentiation GO:0030154 IEA
    cilium organization GO:0044782 IGI
    regulation of cilium assembly GO:1902017 IBA
    regulation of cilium assembly GO:1902017 IMP
Subcellular Localization
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 HDA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    centriole GO:0005814 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    cilium GO:0005929 IDA
    cilium GO:0005929 IDA
    ciliary basal body GO:0036064 IDA
    sperm flagellum GO:0036126 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    sperm midpiece GO:0097225 ISS
    sperm principal piece GO:0097228 ISS
    centriolar subdistal appendage GO:0120103 IDA
 Experiment description of studies that identified ODF2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ODF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC14A  
Proximity Label-MS Homo sapiens
2 MARK4  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
3 NRP1 8829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DNAJC8 22826
Cross-Linking-MS (XL-MS) Homo sapiens
5 SVIL 6840
Two-hybrid Homo sapiens
6 CCDC114  
Affinity Capture-MS Homo sapiens
7 NINL  
Proximity Label-MS Homo sapiens
8 LTBR 4055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SP1  
Affinity Capture-MS Homo sapiens
10 ZDHHC17 23390
Two-hybrid Homo sapiens
11 AURKA 6790
Affinity Capture-MS Homo sapiens
12 FBXO25  
Biochemical Activity Homo sapiens
13 BRCA1 672
Co-localization Homo sapiens
14 RAB3IL1 5866
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ODF2L  
Affinity Capture-MS Homo sapiens
16 Ndc80  
Affinity Capture-MS Mus musculus
17 SMURF1 57154
Biochemical Activity Homo sapiens
18 CEACAM16  
Affinity Capture-MS Homo sapiens
19 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
20 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
21 PLOD1 5351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MAP3K1 4214
Biochemical Activity Homo sapiens
23 CFL1 1072
Cross-Linking-MS (XL-MS) Homo sapiens
24 EPRS 2058
Cross-Linking-MS (XL-MS) Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
26 PRPF38B 55119
Cross-Linking-MS (XL-MS) Homo sapiens
27 TRIM55  
Two-hybrid Homo sapiens
28 RAB8A 4218
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
29 PIBF1  
Affinity Capture-MS Homo sapiens
30 MKRN2 23609
Affinity Capture-RNA Homo sapiens
31 Mta2 23942
Affinity Capture-MS Mus musculus
32 NIN 51199
Proximity Label-MS Homo sapiens
33 CEP135  
Proximity Label-MS Homo sapiens
34 ING5  
Two-hybrid Homo sapiens
35 PLK1 5347
Affinity Capture-MS Homo sapiens
36 CCDC14  
Proximity Label-MS Homo sapiens
37 NDC80 10403
Proximity Label-MS Homo sapiens
38 MEOX2  
Two-hybrid Homo sapiens
39 SNW1 22938
Two-hybrid Homo sapiens
40 IKZF5 64376
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PCM1 5108
Proximity Label-MS Homo sapiens
42 TRIM63  
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here