Gene description for Hyou1
Gene name hypoxia up-regulated 1
Gene symbol Hyou1
Other names/aliases AI415631
CBP-140
Cab140
Grp170
Orp150
Species Mus musculus
 Database cross references - Hyou1
ExoCarta ExoCarta_12282
Vesiclepedia VP_12282
Entrez Gene 12282
UniProt Q9JKR6  
 Hyou1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hyou1
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IBA
    ATP binding GO:0005524 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    response to hypoxia GO:0001666 ISO
    response to ischemia GO:0002931 IDA
    response to ischemia GO:0002931 IDA
    protein folding GO:0006457 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISO
    response to endoplasmic reticulum stress GO:0034976 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    cellular response to hypoxia GO:0071456 IEA
    cellular response to hypoxia GO:0071456 ISO
    negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway GO:1903298 IBA
    negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway GO:1903298 IEA
    negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway GO:1903298 ISO
    negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway GO:1903382 IMP
Subcellular Localization
    extracellular region GO:0005576 TAS
    endoplasmic reticulum lumen GO:0005788 IEA
    smooth endoplasmic reticulum GO:0005790 ISO
    endoplasmic reticulum chaperone complex GO:0034663 IBA
    endoplasmic reticulum chaperone complex GO:0034663 IDA
 Experiment description of studies that identified Hyou1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hyou1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Sec61a1  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
3 Sil1  
Dosage Rescue Mus musculus
Synthetic Haploinsufficiency Mus musculus
4 Stub1 56424
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Lmo2  
Two-hybrid Mus musculus
6 Tmed10 68581
Co-fractionation Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 Hspa5 14828
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Vcp 269523
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 Os9  
Co-fractionation Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Hyou1 is involved
No pathways found





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