Gene description for Stub1
Gene name STIP1 homology and U-Box containing protein 1
Gene symbol Stub1
Other names/aliases 0610033N24Rik
2210017D18Rik
2310040B03Rik
AW046544
Chip
Species Mus musculus
 Database cross references - Stub1
ExoCarta ExoCarta_56424
Vesiclepedia VP_56424
Entrez Gene 56424
UniProt Q9WUD1  
 Stub1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stub1
Molecular Function
    G protein-coupled receptor binding GO:0001664 IEA
    G protein-coupled receptor binding GO:0001664 ISO
    ubiquitin-protein transferase activity GO:0004842 ISO
    ubiquitin-protein transferase activity GO:0004842 ISS
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 ISO
    kinase binding GO:0019900 IEA
    kinase binding GO:0019900 ISO
    Hsp70 protein binding GO:0030544 ISO
    Hsp70 protein binding GO:0030544 ISS
    Hsp70 protein binding GO:0030544 TAS
    protein-macromolecule adaptor activity GO:0030674 TAS
    TPR domain binding GO:0030911 ISO
    TPR domain binding GO:0030911 ISS
    heat shock protein binding GO:0031072 ISO
    heat shock protein binding GO:0031072 TAS
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin-ubiquitin ligase activity GO:0034450 IDA
    protein homodimerization activity GO:0042803 IPI
    protein homodimerization activity GO:0042803 ISO
    SMAD binding GO:0046332 ISO
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 ISO
    misfolded protein binding GO:0051787 IEA
    misfolded protein binding GO:0051787 ISO
    Hsp90 protein binding GO:0051879 ISO
    Hsp90 protein binding GO:0051879 ISS
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 ISO
    R-SMAD binding GO:0070412 IEA
    R-SMAD binding GO:0070412 ISO
Biological Process
    MAPK cascade GO:0000165 ISS
    protein polyubiquitination GO:0000209 IBA
    protein polyubiquitination GO:0000209 IDA
    protein polyubiquitination GO:0000209 ISO
    protein polyubiquitination GO:0000209 ISS
    DNA repair GO:0006281 IEA
    protein folding GO:0006457 TAS
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    protein monoubiquitination GO:0006513 IEA
    protein monoubiquitination GO:0006513 ISO
    protein quality control for misfolded or incompletely synthesized proteins GO:0006515 IBA
    protein quality control for misfolded or incompletely synthesized proteins GO:0006515 IMP
    protein quality control for misfolded or incompletely synthesized proteins GO:0006515 ISO
    negative regulation of cardiac muscle hypertrophy GO:0010614 IEA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 TAS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IEA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 ISO
    endoplasmic reticulum unfolded protein response GO:0030968 IDA
    positive regulation of protein ubiquitination GO:0031398 IDA
    positive regulation of protein ubiquitination GO:0031398 ISO
    positive regulation of protein ubiquitination GO:0031398 ISS
    regulation of protein stability GO:0031647 ISO
    regulation of glucocorticoid metabolic process GO:0031943 ISO
    regulation of glucocorticoid metabolic process GO:0031943 ISS
    negative regulation of protein binding GO:0032091 IDA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISS
    negative regulation of smooth muscle cell apoptotic process GO:0034392 ISO
    negative regulation of smooth muscle cell apoptotic process GO:0034392 ISS
    positive regulation of smooth muscle cell apoptotic process GO:0034393 ISS
    negative regulation of peroxisome proliferator activated receptor signaling pathway GO:0035359 IDA
    ERAD pathway GO:0036503 IEA
    ERAD pathway GO:0036503 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISS
    positive regulation of proteolysis GO:0045862 IBA
    positive regulation of proteolysis GO:0045862 ISO
    positive regulation of proteolysis GO:0045862 ISS
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    positive regulation of ubiquitin-protein transferase activity GO:0051443 IDA
    protein maturation GO:0051604 TAS
    protein autoubiquitination GO:0051865 ISO
    protein autoubiquitination GO:0051865 ISS
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K63-linked ubiquitination GO:0070534 ISS
    cellular response to misfolded protein GO:0071218 IBA
    cellular response to misfolded protein GO:0071218 IMP
    cellular response to misfolded protein GO:0071218 ISO
    positive regulation of chaperone-mediated protein complex assembly GO:0090035 IEA
    positive regulation of chaperone-mediated protein complex assembly GO:0090035 ISO
    positive regulation of mitophagy GO:1901526 ISS
    positive regulation of ERAD pathway GO:1904294 ISO
    positive regulation of ERAD pathway GO:1904294 ISS
    negative regulation of vascular associated smooth muscle contraction GO:1904694 IEA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 ISO
    ubiquitin ligase complex GO:0000151 ISS
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 ISS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Z disc GO:0030018 IBA
    Z disc GO:0030018 IDA
    ubiquitin conjugating enzyme complex GO:0031371 TAS
    nuclear inclusion body GO:0042405 IEA
    nuclear inclusion body GO:0042405 ISO
    protein folding chaperone complex GO:0101031 IEA
    protein folding chaperone complex GO:0101031 ISO
 Experiment description of studies that identified Stub1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stub1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CUL1 8454
Affinity Capture-Western Homo sapiens
2 UBE2N 7334
Co-crystal Structure Homo sapiens
3 Akt1  
Affinity Capture-Western Mus musculus
4 Fitm2  
Negative Genetic Mus musculus
5 Hspa8 15481
Co-fractionation Mus musculus
6 Dyx1c1  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
7 SOD1 6647
Affinity Capture-Western Homo sapiens
8 Ube2d1  
Co-fractionation Mus musculus
9 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
10 Il4ra  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 Cdk5 12568
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
12 Tssc1  
Co-fractionation Mus musculus
13 Mapt  
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
14 Stub1 56424
Co-crystal Structure Mus musculus
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
Co-crystal Structure Mus musculus
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
15 Foxp3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
16 SKP2  
Affinity Capture-Western Homo sapiens
17 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
18 MAP3K2 10746
Two-hybrid Homo sapiens
19 Hspa1b 15511
Affinity Capture-Western Mus musculus
20 Chaf1a  
Affinity Capture-MS Mus musculus
21 PLEKHO1 51177
Two-hybrid Homo sapiens
22 Ube2n 93765
Co-fractionation Mus musculus
23 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
24 Lrrk2  
Affinity Capture-Western Mus musculus
25 UBE2V1 7335
Co-crystal Structure Homo sapiens
26 Cand1 71902
Co-fractionation Mus musculus
27 DYX1C1  
Two-hybrid Homo sapiens
28 Atxn3  
Affinity Capture-Western Mus musculus
29 Ubxn2a  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
30 Atcay  
Co-fractionation Mus musculus
31 Map3k14  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
32 Bag3  
Co-fractionation Mus musculus
33 Traf6  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
34 Chrna3  
Affinity Capture-Western Mus musculus
35 Atg5  
Affinity Capture-RNA Mus musculus
36 Josd2  
Co-fractionation Mus musculus
37 Cdc37l1  
Co-fractionation Mus musculus
38 Nanog  
Affinity Capture-MS Mus musculus
39 Twist1  
Proximity Label-MS Mus musculus
40 Bag4  
Co-fractionation Mus musculus
41 Bag5 70369
Affinity Capture-Western Mus musculus
42 Sp7  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
43 HSPA4 3308
Affinity Capture-Western Homo sapiens
44 Sec61a1  
Affinity Capture-Western Mus musculus
45 Map3k2  
Affinity Capture-Western Mus musculus
46 Aifm1 83533
Affinity Capture-Western Rattus norvegicus
47 Bag1  
Co-fractionation Mus musculus
48 App 11820
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
49 UBE2D3 7323
Reconstituted Complex Homo sapiens
50 Fbxo2  
Co-fractionation Mus musculus
51 Traf3  
Affinity Capture-Western Mus musculus
52 Hspa9 15526
Affinity Capture-Western Mus musculus
53 Hyou1 12282
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
54 BMPR1B 658
Affinity Capture-Luminescence Homo sapiens
55 Fancd2  
Affinity Capture-MS Mus musculus
56 Tcf3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
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