Gene description for Hspa9
Gene name heat shock protein 9
Gene symbol Hspa9
Other names/aliases 74kDa
Csa
Grp75
Hsc74
Hsp74
Hsp74a
Hspa9a
Mortalin
Mot-2
Mot2
Mthsp70
Pbp74
Species Mus musculus
 Database cross references - Hspa9
ExoCarta ExoCarta_15526
Vesiclepedia VP_15526
Entrez Gene 15526
UniProt P38647  
 Hspa9 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hspa9
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    fibroblast growth factor binding GO:0017134 ISO
    enzyme binding GO:0019899 IPI
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein folding chaperone GO:0044183 IBA
    unfolded protein binding GO:0051082 IEA
    protein-folding chaperone binding GO:0051087 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IEA
    protein export from nucleus GO:0006611 IDA
    iron-sulfur cluster assembly GO:0016226 IBA
    iron-sulfur cluster assembly GO:0016226 IMP
    iron-sulfur cluster assembly GO:0016226 ISO
    erythrocyte differentiation GO:0030218 ISO
    erythrocyte differentiation GO:0030218 ISS
    calcium import into the mitochondrion GO:0036444 ISS
    protein refolding GO:0042026 IBA
    positive regulation of apoptotic process GO:0043065 IEA
    regulation of erythrocyte differentiation GO:0045646 ISO
    regulation of erythrocyte differentiation GO:0045646 ISS
    negative regulation of erythrocyte differentiation GO:0045647 ISO
    negative regulation of erythrocyte differentiation GO:0045647 ISS
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    negative regulation of hematopoietic stem cell differentiation GO:1902037 IMP
    negative regulation of hemopoiesis GO:1903707 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 TAS
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 ISO
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrial nucleoid GO:0042645 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Hspa9 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hspa9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Elf5  
Affinity Capture-MS Mus musculus
3 SNCA 6622
Affinity Capture-MS Homo sapiens
4 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
5 Hspd1 15510
Co-fractionation Mus musculus
6 Mycbp2 105689
Affinity Capture-MS Mus musculus
7 Tubgcp2  
Co-fractionation Mus musculus
8 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
9 Sirt3  
Co-fractionation Mus musculus
10 Spg20  
Co-fractionation Mus musculus
11 MTNR1B  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
12 Kcnma1  
Affinity Capture-MS Mus musculus
13 Ubxn2a  
Affinity Capture-Western Mus musculus
14 Stub1 56424
Affinity Capture-Western Mus musculus
15 Kcna3  
Affinity Capture-MS Mus musculus
16 Kctd13  
Affinity Capture-MS Mus musculus
17 Trap1 68015
Cross-Linking-MS (XL-MS) Mus musculus
18 Ykt6 56418
Co-fractionation Mus musculus
19 Tfe3  
Affinity Capture-MS Mus musculus
20 MTNR1A  
Reconstituted Complex Homo sapiens
21 Tia1  
Affinity Capture-MS Mus musculus
22 Dnaja3  
Affinity Capture-MS Mus musculus
23 Mks1  
Affinity Capture-MS Mus musculus
24 Iqcb1  
Affinity Capture-MS Mus musculus
25 Ywhaz 22631
Affinity Capture-MS Mus musculus
26 Mettl21e  
Affinity Capture-MS Mus musculus
27 Nphp4  
Affinity Capture-MS Mus musculus
28 Fzr1  
Affinity Capture-MS Mus musculus
29 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
30 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
31 Sdha 66945
Co-fractionation Mus musculus
32 Unk  
Affinity Capture-RNA Mus musculus
33 Tnfaip8  
Co-fractionation Mus musculus
34 HACE1 57531
Affinity Capture-MS Homo sapiens
35 Invs  
Affinity Capture-MS Mus musculus
36 Atxn1  
Proximity Label-MS Mus musculus
37 Grpel1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
38 Eed  
Affinity Capture-MS Mus musculus
39 Hspa9 15526
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
40 Ahi1  
Affinity Capture-MS Mus musculus
41 Atg16l1  
Affinity Capture-MS Mus musculus
42 Nphp1  
Affinity Capture-MS Mus musculus
43 Fancd2  
Affinity Capture-MS Mus musculus
44 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
45 Tmem173  
Proximity Label-MS Mus musculus
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