Gene description for Aifm1
Gene name apoptosis-inducing factor, mitochondrion-associated 1
Gene symbol Aifm1
Other names/aliases Aif
Pdcd8
Species Rattus norvegicus
 Database cross references - Aifm1
ExoCarta ExoCarta_83533
Vesiclepedia VP_83533
Entrez Gene 83533
UniProt Q9JM53  
 Aifm1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Aifm1
Molecular Function
    DNA binding GO:0003677 ISO
    DNA binding GO:0003677 ISS
    NADH dehydrogenase activity GO:0003954 IEA
    NAD(P)H oxidase H2O2-forming activity GO:0016174 IBA
    NAD(P)H oxidase H2O2-forming activity GO:0016174 ISO
    NAD(P)H oxidase H2O2-forming activity GO:0016174 ISS
    oxidoreductase activity GO:0016491 IEA
    oxidoreductase activity, acting on NAD(P)H GO:0016651 ISO
    oxidoreductase activity, acting on NAD(P)H GO:0016651 ISS
    protein dimerization activity GO:0046983 IEA
    FAD binding GO:0071949 IBA
    FAD binding GO:0071949 ISO
    FAD binding GO:0071949 ISS
    poly-ADP-D-ribose binding GO:0072572 ISO
    poly-ADP-D-ribose binding GO:0072572 ISS
Biological Process
    response to ischemia GO:0002931 IEP
    response to ischemia GO:0002931 IEP
    apoptotic process GO:0006915 IBA
    apoptotic process GO:0006915 IEA
    apoptotic process GO:0006915 ISO
    response to toxic substance GO:0009636 IEP
    neuron differentiation GO:0030182 ISO
    mitochondrial respiratory chain complex I assembly GO:0032981 ISO
    mitochondrial respiratory chain complex assembly GO:0033108 IBA
    mitochondrial respiratory chain complex assembly GO:0033108 ISO
    mitochondrial respiratory chain complex assembly GO:0033108 ISS
    positive regulation of neuron apoptotic process GO:0043525 IMP
    protein import into mitochondrial intermembrane space GO:0045041 ISO
    protein import into mitochondrial intermembrane space GO:0045041 ISS
    neuron apoptotic process GO:0051402 ISO
    positive regulation of necroptotic process GO:0060545 ISO
    positive regulation of necroptotic process GO:0060545 ISS
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 ISO
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 ISS
    cellular response to hydrogen peroxide GO:0070301 IEP
    cellular response to estradiol stimulus GO:0071392 IEP
    cellular response to hypoxia GO:0071456 IEP
    cellular response to nitric oxide GO:0071732 IEP
    response to L-glutamate GO:1902065 IEP
    regulation of apoptotic DNA fragmentation GO:1902510 ISO
    cellular response to aldosterone GO:1904045 IEP
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial intermembrane space GO:0005758 ISO
    mitochondrial intermembrane space GO:0005758 ISS
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified Aifm1 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Aifm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Stub1 56424
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Aifm1 is involved
No pathways found





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