Gene description for Cacybp
Gene name calcyclin binding protein
Gene symbol Cacybp
Other names/aliases SIP
Species Mus musculus
 Database cross references - Cacybp
ExoCarta ExoCarta_12301
Vesiclepedia VP_12301
Entrez Gene 12301
UniProt Q9CXW3  
 Cacybp identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cacybp
Molecular Function
    protein binding GO:0005515 IPI
    tubulin binding GO:0015631 IEA
    protein domain specific binding GO:0019904 IPI
    ubiquitin protein ligase binding GO:0031625 EXP
    ubiquitin protein ligase binding GO:0031625 IPI
    protein homodimerization activity GO:0042803 ISO
    S100 protein binding GO:0044548 IEA
    molecular adaptor activity GO:0060090 EXP
Biological Process
    heart development GO:0007507 IBA
    positive regulation of DNA replication GO:0045740 ISO
    cardiac muscle cell differentiation GO:0055007 ISO
    response to growth hormone GO:0060416 ISO
    cellular response to leukemia inhibitory factor GO:1990830 IEP
Subcellular Localization
    nucleus GO:0005634 IBA
    nuclear envelope lumen GO:0005641 IDA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    SCF ubiquitin ligase complex GO:0019005 IMP
    beta-catenin destruction complex GO:0030877 ISO
    beta-catenin destruction complex GO:0030877 ISS
    cell body GO:0044297 ISO
 Experiment description of studies that identified Cacybp in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cacybp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 S100a1  
Affinity Capture-Western Mus musculus
2 Tpd52  
Co-fractionation Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Ndn  
Two-hybrid Mus musculus
7 S100a6 20200
Affinity Capture-Western Mus musculus
8 Skp1a 21402
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
9 Atg16l1  
Affinity Capture-MS Mus musculus
10 Rpap3  
Co-fractionation Mus musculus
11 Siah1a  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Cacybp is involved
No pathways found





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