Gene description for Capn1
Gene name calpain 1
Gene symbol Capn1
Other names/aliases Capa-1
Capa1
mu-calpin
Species Mus musculus
 Database cross references - Capn1
ExoCarta ExoCarta_12333
Vesiclepedia VP_12333
Entrez Gene 12333
UniProt O35350  
 Capn1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Capn1
Molecular Function
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 IBA
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 IDA
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 ISO
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 TAS
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 ISO
    peptidase activity GO:0008233 ISO
    enzyme binding GO:0019899 ISO
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IMP
    proteolysis GO:0006508 ISO
    proteolysis GO:0006508 ISS
    proteolysis GO:0006508 NAS
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 ISO
    protein autoprocessing GO:0016540 ISO
    protein catabolic process GO:0030163 ISO
    negative regulation of actin filament polymerization GO:0030837 ISO
    receptor catabolic process GO:0032801 IDA
    positive regulation of vascular permeability GO:0043117 ISO
    regulation of catalytic activity GO:0050790 ISO
    regulation of catalytic activity GO:0050790 ISS
    mammary gland involution GO:0060056 IMP
    cellular response to hydrogen peroxide GO:0070301 ISO
    self proteolysis GO:0097264 ISO
    self proteolysis GO:0097264 ISS
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 ISO
    positive regulation of leukocyte tethering or rolling GO:1903238 ISO
    response to angiotensin GO:1990776 ISO
Subcellular Localization
    cornified envelope GO:0001533 IDA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IDA
    lysosome GO:0005764 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 ISO
    calpain complex GO:0110158 ISS
 Experiment description of studies that identified Capn1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Capn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ptpn5  
Biochemical Activity Mus musculus
2 Uggt1 320011
Co-fractionation Mus musculus
3 Fzr1  
Affinity Capture-MS Mus musculus
4 Sdf2  
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Rps6ka1 20111
Co-fractionation Mus musculus
7 Os9  
Co-fractionation Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here