Gene description for Capzb
Gene name capping protein (actin filament) muscle Z-line, beta
Gene symbol Capzb
Other names/aliases 1700120C01Rik
AI325129
CPB1
CPB2
CPbeat2
CPbeta1
CPbeta2
Cappb1
Species Mus musculus
 Database cross references - Capzb
ExoCarta ExoCarta_12345
Vesiclepedia VP_12345
Entrez Gene 12345
UniProt P47757  
 Capzb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Capzb
Molecular Function
    actin binding GO:0003779 IEA
    actin binding GO:0003779 ISO
    protein binding GO:0005515 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    cell morphogenesis GO:0000902 IBA
    cytoskeleton organization GO:0007010 ISO
    cytoskeleton organization GO:0007010 ISS
    actin polymerization or depolymerization GO:0008154 IDA
    regulation of lamellipodium assembly GO:0010591 IBA
    regulation of cell morphogenesis GO:0022604 ISO
    regulation of cell morphogenesis GO:0022604 ISS
    cell projection organization GO:0030030 IMP
    lamellipodium assembly GO:0030032 IMP
    barbed-end actin filament capping GO:0051016 IBA
    barbed-end actin filament capping GO:0051016 IEA
    negative regulation of filopodium assembly GO:0051490 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    brush border GO:0005903 IDA
    F-actin capping protein complex GO:0008290 IBA
    F-actin capping protein complex GO:0008290 IEA
    postsynaptic density GO:0014069 IDA
    membrane GO:0016020 IDA
    Z disc GO:0030018 IEA
    lamellipodium GO:0030027 IDA
    cortical cytoskeleton GO:0030863 IDA
    asymmetric synapse GO:0032279 IDA
    cytoskeletal calyx GO:0033150 IEA
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 ISO
    WASH complex GO:0071203 ISO
    WASH complex GO:0071203 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IDA
    sperm head-tail coupling apparatus GO:0120212 IDA
 Experiment description of studies that identified Capzb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Capzb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
5 Ap3b1  
Co-fractionation Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Myo6  
Affinity Capture-Western Mus musculus
8 Ywhae 22627
Affinity Capture-MS Mus musculus
9 Mapt  
Affinity Capture-MS Mus musculus
10 Capza2 12343
Cross-Linking-MS (XL-MS) Mus musculus
11 Mtpn  
Co-fractionation Mus musculus
12 Fkbp15  
Co-fractionation Mus musculus
13 Lcp1 18826
Co-fractionation Mus musculus
14 Bag3  
Affinity Capture-Western Mus musculus
15 Capzb 12345
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
16 Nanog  
Affinity Capture-MS Mus musculus
17 Fancd2  
Affinity Capture-MS Mus musculus
18 E430025E21Rik  
Co-fractionation Mus musculus
19 Ywhaz 22631
Affinity Capture-MS Mus musculus
20 Snx27 76742
Co-fractionation Mus musculus
21 HACE1 57531
Affinity Capture-MS Homo sapiens
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