Gene description for Ctsb
Gene name cathepsin B
Gene symbol Ctsb
Other names/aliases CB
Species Mus musculus
 Database cross references - Ctsb
ExoCarta ExoCarta_13030
Vesiclepedia VP_13030
Entrez Gene 13030
UniProt P10605  
 Ctsb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ctsb
Molecular Function
    endopeptidase activity GO:0004175 IMP
    endopeptidase activity GO:0004175 ISO
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type endopeptidase activity GO:0004197 ISO
    collagen binding GO:0005518 IEA
    collagen binding GO:0005518 ISO
    peptidase activity GO:0008233 ISO
    cysteine-type peptidase activity GO:0008234 IDA
    cysteine-type peptidase activity GO:0008234 ISO
    kininogen binding GO:0030984 ISO
    peptide binding GO:0042277 ISO
    identical protein binding GO:0042802 ISO
    proteoglycan binding GO:0043394 IEA
    proteoglycan binding GO:0043394 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    proteolysis GO:0006508 ISO
    thyroid hormone generation GO:0006590 IMP
    protein catabolic process GO:0030163 ISO
    collagen catabolic process GO:0030574 IEA
    collagen catabolic process GO:0030574 ISO
    epithelial cell differentiation GO:0030855 IEA
    decidualization GO:0046697 IGI
    symbiont entry into host cell GO:0046718 IMP
    neuron apoptotic process GO:0051402 ISO
    proteolysis involved in protein catabolic process GO:0051603 IBA
    proteolysis involved in protein catabolic process GO:0051603 IMP
    proteolysis involved in protein catabolic process GO:0051603 ISO
    cellular response to thyroid hormone stimulus GO:0097067 IEA
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    mitochondrion GO:0005739 HDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosome GO:0005764 ISO
    caveola GO:0005901 ISO
    external side of plasma membrane GO:0009897 IDA
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 ISO
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    sarcolemma GO:0042383 ISO
    melanosome GO:0042470 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    peptidase inhibitor complex GO:1904090 ISO
 Experiment description of studies that identified Ctsb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ctsb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hba-a1 15122
Biochemical Activity Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Hbb 15127
Biochemical Activity Mus musculus
4 Unk  
Affinity Capture-RNA Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here