Gene description for Dab2
Gene name disabled 2, mitogen-responsive phosphoprotein
Gene symbol Dab2
Other names/aliases 5730435J12Rik
AA960054
AI957090
D15Wsu122e
D630005B22Rik
Doc-2
Doc2
p96
Species Mus musculus
 Database cross references - Dab2
ExoCarta ExoCarta_13132
Vesiclepedia VP_13132
Entrez Gene 13132
UniProt P98078  
 Dab2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dab2
Molecular Function
    integrin binding GO:0005178 IMP
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    clathrin binding GO:0030276 IDA
    phosphatidylinositol binding GO:0035091 IDA
    AP-2 adaptor complex binding GO:0035612 IDA
    clathrin adaptor activity GO:0035615 IBA
    clathrin adaptor activity GO:0035615 IDA
    clathrin adaptor activity GO:0035615 ISO
    cargo receptor activity GO:0038024 IBA
    cargo receptor activity GO:0038024 IDA
    cargo receptor activity GO:0038024 ISO
    SMAD binding GO:0046332 ISO
    low-density lipoprotein particle receptor binding GO:0050750 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    cell morphogenesis GO:0000902 IMP
    in utero embryonic development GO:0001701 IMP
    positive regulation of receptor recycling GO:0001921 IMP
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of receptor internalization GO:0002092 IMP
    endocytosis GO:0006897 IEA
    receptor-mediated endocytosis GO:0006898 IBA
    receptor-mediated endocytosis GO:0006898 IPI
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    positive regulation of epithelial to mesenchymal transition GO:0010718 IBA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IMP
    positive regulation of epithelial to mesenchymal transition GO:0010718 ISO
    negative regulation of neuron projection development GO:0010977 ISO
    protein transport GO:0015031 IEA
    Wnt signaling pathway GO:0016055 IEA
    myeloid cell differentiation GO:0030099 IEP
    negative regulation of cell growth GO:0030308 ISO
    positive regulation of cell migration GO:0030335 IMP
    positive regulation of cell migration GO:0030335 ISO
    negative regulation of protein binding GO:0032091 ISO
    positive regulation of aldosterone biosynthetic process GO:0032349 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IMP
    leading edge cell differentiation GO:0035026 ISO
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of cell adhesion GO:0045785 IDA
    positive regulation of cell adhesion GO:0045785 IMP
    positive regulation of endocytosis GO:0045807 IBA
    positive regulation of endocytosis GO:0045807 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of JNK cascade GO:0046330 IDA
    clathrin coat assembly GO:0048268 IDA
    clathrin coat assembly GO:0048268 ISO
    response to steroid hormone GO:0048545 ISO
    negative regulation of epithelial cell proliferation GO:0050680 ISO
    positive regulation of SMAD protein signal transduction GO:0060391 ISO
    negative regulation of androgen receptor signaling pathway GO:0060766 ISO
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    hematopoietic stem cell proliferation GO:0071425 IDA
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IBA
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISO
    renal protein absorption GO:0097017 IMP
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IDA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    negative regulation of protein localization to plasma membrane GO:1903077 IMP
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
    positive regulation of Wnt signaling pathway, planar cell polarity pathway GO:2000096 ISO
    positive regulation of clathrin-dependent endocytosis GO:2000370 IDA
    positive regulation of clathrin-dependent endocytosis GO:2000370 ISO
    positive regulation of early endosome to late endosome transport GO:2000643 ISO
    positive regulation of aldosterone secretion GO:2000860 ISO
    positive regulation of integrin-mediated signaling pathway GO:2001046 IMP
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
Subcellular Localization
    fibrillar center GO:0001650 ISO
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    clathrin-coated pit GO:0005905 IBA
    clathrin-coated pit GO:0005905 ISO
    apical plasma membrane GO:0016324 IDA
    clathrin coat of coated pit GO:0030132 IDA
    clathrin-coated vesicle GO:0030136 ISO
    clathrin-coated vesicle membrane GO:0030665 IEA
    clathrin-coated vesicle membrane GO:0030665 ISO
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Dab2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dab2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cltc 54241
Affinity Capture-Western Rattus norvegicus
2 Ap2m1 116563
Affinity Capture-Western Rattus norvegicus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Mmp2  
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 ITSN1 6453
Affinity Capture-Western Homo sapiens
7 Ap2a2  
Affinity Capture-Western Rattus norvegicus
8 EPS15 2060
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 Ap2a1  
Affinity Capture-Western Rattus norvegicus
10 Atg5  
Affinity Capture-MS Mus musculus
11 Clta 83800
Affinity Capture-Western Rattus norvegicus
12 Tsc22d3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
13 Nostrin 329416
Co-fractionation Mus musculus
14 Ap2b1 140670
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here