Gene description for Cltc
Gene name clathrin, heavy chain (Hc)
Gene symbol Cltc
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Cltc
ExoCarta ExoCarta_54241
Vesiclepedia VP_54241
Entrez Gene 54241
UniProt P11442  
 Cltc identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Cortical neurones 16446100    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Cltc
Molecular Function
    double-stranded RNA binding GO:0003725 IEA
    double-stranded RNA binding GO:0003725 ISO
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 ISO
    ankyrin binding GO:0030506 IDA
    heat shock protein binding GO:0031072 IDA
    clathrin light chain binding GO:0032051 IBA
    clathrin light chain binding GO:0032051 IEA
    clathrin light chain binding GO:0032051 ISO
    peptide binding GO:0042277 IDA
    low-density lipoprotein particle receptor binding GO:0050750 IEA
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    disordered domain specific binding GO:0097718 IEA
    disordered domain specific binding GO:0097718 ISO
    protein serine/threonine kinase binding GO:0120283 IPI
    ubiquitin-specific protease binding GO:1990381 IEA
    ubiquitin-specific protease binding GO:1990381 ISO
Biological Process
    mitotic cell cycle GO:0000278 IBA
    mitotic cell cycle GO:0000278 IEA
    mitotic cell cycle GO:0000278 IMP
    mitotic cell cycle GO:0000278 ISO
    intracellular protein transport GO:0006886 IEA
    receptor-mediated endocytosis GO:0006898 IBA
    receptor-mediated endocytosis GO:0006898 IMP
    receptor-mediated endocytosis GO:0006898 ISO
    autophagy GO:0006914 IEA
    Golgi organization GO:0007030 IMP
    vesicle-mediated transport GO:0016192 IEA
    receptor internalization GO:0031623 IEA
    receptor internalization GO:0031623 ISO
    transferrin transport GO:0033572 IEA
    transferrin transport GO:0033572 ISO
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    clathrin coat assembly GO:0048268 IBA
    clathrin coat assembly GO:0048268 IEA
    clathrin coat assembly GO:0048268 ISO
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    regulation of mitotic spindle organization GO:0060236 IEA
    regulation of mitotic spindle organization GO:0060236 ISO
    clathrin coat disassembly GO:0072318 ISS
    clathrin-dependent endocytosis GO:0072583 IEA
    clathrin-dependent endocytosis GO:0072583 ISO
    mitotic spindle assembly GO:0090307 ISO
    amyloid-beta clearance by transcytosis GO:0150093 IEA
    amyloid-beta clearance by transcytosis GO:0150093 ISO
    negative regulation of hyaluronan biosynthetic process GO:1900126 IEA
    negative regulation of hyaluronan biosynthetic process GO:1900126 ISO
    negative regulation of hyaluronan biosynthetic process GO:1900126 ISS
    negative regulation of protein localization to plasma membrane GO:1903077 IEA
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
Subcellular Localization
    lysosome GO:0005764 IEA
    lysosome GO:0005764 ISO
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    spindle GO:0005819 IBA
    spindle GO:0005819 IDA
    spindle GO:0005819 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    clathrin-coated pit GO:0005905 ISO
    membrane GO:0016020 ISO
    membrane coat GO:0030117 ISO
    clathrin coat GO:0030118 IDA
    clathrin coat GO:0030118 ISO
    clathrin coat GO:0030118 TAS
    clathrin coat of trans-Golgi network vesicle GO:0030130 IEA
    clathrin coat of coated pit GO:0030132 IEA
    clathrin-coated vesicle GO:0030136 ISO
    T-tubule GO:0030315 IDA
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    Myb complex GO:0031523 ISO
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 IDA
    melanosome GO:0042470 IEA
    terminal bouton GO:0043195 HDA
    clathrin-coated endocytic vesicle GO:0045334 IBA
    clathrin complex GO:0071439 IBA
    clathrin complex GO:0071439 IEA
    clathrin complex GO:0071439 ISO
    mitotic spindle GO:0072686 ISO
    photoreceptor ribbon synapse GO:0098684 ISO
    presynaptic endocytic zone membrane GO:0098835 ISO
    postsynaptic endocytic zone GO:0098843 ISO
    extrinsic component of synaptic vesicle membrane GO:0098850 IDA
    glutamatergic synapse GO:0098978 ISO
    mitotic spindle microtubule GO:1990498 IEA
    mitotic spindle microtubule GO:1990498 ISO
 Experiment description of studies that identified Cltc in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 30
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
4
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cltc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Epn1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
2 Gja1  
Affinity Capture-MS Rattus norvegicus
3 Gabarap  
Affinity Capture-MS Rattus norvegicus
Reconstituted Complex Rattus norvegicus
4 Snca  
Affinity Capture-Western Rattus norvegicus
5 Ncald  
Affinity Capture-MS Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
6 Amph  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
7 Fbxo21  
Affinity Capture-MS Rattus norvegicus
8 Dab2 13132
Affinity Capture-Western Mus musculus
9 Bin1  
Affinity Capture-Western Rattus norvegicus
10 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
11 Sumo3  
Affinity Capture-MS Rattus norvegicus
12 Arfgap1  
Affinity Capture-MS Rattus norvegicus
Reconstituted Complex Rattus norvegicus
13 Park7 117287
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
14 Ap2b1 140670
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Cltc is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
Formation of annular gap junctions IEA Reactome
Gap junction degradation IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Golgi Associated Vesicle Biogenesis IEA Reactome
Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Lysosome Vesicle Biogenesis IEA Reactome
Membrane Trafficking IEA Reactome
MHC class II antigen presentation IEA Reactome
Nervous system development IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
RHO GTPase cycle IEA Reactome
RHOU GTPase cycle IEA Reactome
RHOV GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
trans-Golgi Network Vesicle Budding IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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