Gene description for Mapre1
Gene name microtubule-associated protein, RP/EB family, member 1
Gene symbol Mapre1
Other names/aliases 5530600P05Rik
AI462499
AI504412
AW260097
BIM1p
D2Ertd459e
Eb1
Species Mus musculus
 Database cross references - Mapre1
ExoCarta ExoCarta_13589
Vesiclepedia VP_13589
Entrez Gene 13589
UniProt Q61166  
 Mapre1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mapre1
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    microtubule plus-end binding GO:0051010 IBA
    microtubule plus-end binding GO:0051010 IDA
    microtubule plus-end binding GO:0051010 ISO
    microtubule plus-end binding GO:0051010 ISS
Biological Process
    establishment of mitotic spindle orientation GO:0000132 ISO
    establishment of mitotic spindle orientation GO:0000132 ISS
    microtubule bundle formation GO:0001578 IDA
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    regulation of microtubule polymerization or depolymerization GO:0031110 IBA
    negative regulation of microtubule polymerization GO:0031115 ISO
    negative regulation of microtubule polymerization GO:0031115 ISS
    positive regulation of microtubule polymerization GO:0031116 IEA
    positive regulation of microtubule polymerization GO:0031116 ISO
    protein localization to microtubule GO:0035372 IBA
    protein localization to microtubule GO:0035372 IMP
    protein localization to microtubule GO:0035372 ISO
    protein localization to microtubule GO:0035372 ISS
    microtubule polymerization GO:0046785 IDA
    spindle assembly GO:0051225 IBA
    cell division GO:0051301 IEA
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 ISO
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 ISS
    protein localization to centrosome GO:0071539 IMP
    protein localization to mitotic spindle GO:1902480 IEA
    protein localization to astral microtubule GO:1902888 ISO
    protein localization to astral microtubule GO:1902888 ISS
    positive regulation of microtubule plus-end binding GO:1903033 IDA
    positive regulation of microtubule plus-end binding GO:1903033 IGI
    non-motile cilium assembly GO:1905515 IMP
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    microtubule organizing center GO:0005815 IBA
    spindle GO:0005819 ISO
    spindle GO:0005819 ISS
    microtubule GO:0005874 IDA
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISS
    cytoplasmic microtubule GO:0005881 IBA
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    microtubule cytoskeleton GO:0015630 IDA
    cortical microtubule cytoskeleton GO:0030981 ISO
    cortical microtubule cytoskeleton GO:0030981 ISS
    cell projection membrane GO:0031253 IDA
    microtubule plus-end GO:0035371 IBA
    microtubule plus-end GO:0035371 IDA
    ciliary basal body GO:0036064 IDA
    cell projection GO:0042995 IDA
    spindle midzone GO:0051233 IBA
    mitotic spindle pole GO:0097431 ISO
    mitotic spindle pole GO:0097431 ISS
    mitotic spindle astral microtubule end GO:1905721 IEA
    mitotic spindle astral microtubule end GO:1905721 ISO
    mitotic spindle microtubule GO:1990498 IEA
 Experiment description of studies that identified Mapre1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mapre1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK5RAP2 55755
Affinity Capture-MS Homo sapiens
2 DST 667
Affinity Capture-MS Homo sapiens
3 CLIP1 6249
Affinity Capture-MS Homo sapiens
4 SVIL 6840
Affinity Capture-MS Homo sapiens
5 CLASP2 23122
Affinity Capture-MS Homo sapiens
6 KIAA2013 90231
Affinity Capture-MS Homo sapiens
7 SPAG5 10615
Affinity Capture-MS Homo sapiens
8 AKAP9 10142
Affinity Capture-MS Homo sapiens
9 PRPF6 24148
Two-hybrid Homo sapiens
10 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
11 Cnn3 71994
Two-hybrid Mus musculus
12 MRPL37 51253
Affinity Capture-MS Homo sapiens
13 GTSE1 51512
Two-hybrid Homo sapiens
14 TMOD1  
Affinity Capture-MS Homo sapiens
15 SPTBN1 6711
Affinity Capture-MS Homo sapiens
16 NXF1 10482
Two-hybrid Homo sapiens
17 Tmem173  
Proximity Label-MS Mus musculus
18 CLASP1 23332
Affinity Capture-MS Homo sapiens
19 PRKACA 5566
Affinity Capture-MS Homo sapiens
20 TPM1 7168
Affinity Capture-MS Homo sapiens
21 INF2 64423
Affinity Capture-MS Homo sapiens
22 KIF18B 146909
Affinity Capture-MS Homo sapiens
23 Unk  
Affinity Capture-RNA Mus musculus
24 SYBU  
Affinity Capture-MS Homo sapiens
25 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
26 FLII 2314
Affinity Capture-MS Homo sapiens
27 Clip2  
Two-hybrid Mus musculus
28 GAS2L1 10634
Affinity Capture-MS Homo sapiens
29 MBNL2 10150
Two-hybrid Homo sapiens
30 TPM4 7171
Affinity Capture-MS Homo sapiens
31 PRKACB 5567
Affinity Capture-MS Homo sapiens
32 ZNF830  
Two-hybrid Homo sapiens
33 Nono 53610
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Mapre1 is involved
PathwayEvidenceSource
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
G2/M Transition IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome





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