Gene description for AKAP9
Gene name A kinase (PRKA) anchor protein 9
Gene symbol AKAP9
Other names/aliases AKAP-9
AKAP350
AKAP450
CG-NAP
HYPERION
LQT11
MU-RMS-40.16A
PPP1R45
PRKA9
YOTIAO
Species Homo sapiens
 Database cross references - AKAP9
ExoCarta ExoCarta_10142
Vesiclepedia VP_10142
Entrez Gene 10142
HGNC 379
MIM 604001
UniProt Q99996  
 AKAP9 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Malignant pleural effusions 14975938    
 Gene ontology annotations for AKAP9
Molecular Function
    DNA binding GO:0003677 IEA
    signaling receptor binding GO:0005102 TAS
    protein binding GO:0005515 IPI
    potassium channel regulator activity GO:0015459 IBA
    potassium channel regulator activity GO:0015459 IMP
    protein kinase A regulatory subunit binding GO:0034237 IBA
    protein kinase A regulatory subunit binding GO:0034237 IDA
    protein kinase A regulatory subunit binding GO:0034237 IPI
    transmembrane transporter binding GO:0044325 IPI
    molecular adaptor activity GO:0060090 IBA
    molecular adaptor activity GO:0060090 IDA
Biological Process
    microtubule nucleation GO:0007020 IBA
    microtubule nucleation GO:0007020 IMP
    signal transduction GO:0007165 IEA
    chemical synaptic transmission GO:0007268 TAS
    positive regulation of microtubule polymerization GO:0031116 IMP
    protein-containing complex localization GO:0031503 IDA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    response to electrical stimulus GO:0051602 IEA
    maintenance of centrosome location GO:0051661 IBA
    maintenance of centrosome location GO:0051661 IMP
    regulation of membrane repolarization GO:0060306 IMP
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 IMP
    cellular response to cAMP GO:0071320 IMP
    regulation of heart rate by cardiac conduction GO:0086091 IMP
    regulation of cardiac muscle cell action potential involved in regulation of contraction GO:0098909 IMP
    positive regulation of potassium ion transmembrane transporter activity GO:1901018 IMP
    regulation of Golgi organization GO:1903358 IDA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    Golgi stack GO:0005795 IBA
    Golgi stack GO:0005795 IDA
    cis-Golgi network GO:0005801 IBA
    cis-Golgi network GO:0005801 IDA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    voltage-gated potassium channel complex GO:0008076 IDA
    potassium channel complex GO:0034705 IDA
    neuronal cell body GO:0043025 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    dendritic branch GO:0044307 IEA
    synaptic membrane GO:0097060 IBA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    extrinsic component of postsynaptic density membrane GO:0099147 IEA
 Experiment description of studies that identified AKAP9 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 51
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
 Protein-protein interactions for AKAP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC71  
Affinity Capture-MS Homo sapiens
2 TUBGCP3 10426
Affinity Capture-Western Homo sapiens
3 Csnk1d  
Two-hybrid Rattus norvegicus
Biochemical Activity Rattus norvegicus
4 ABCF3 55324
Affinity Capture-MS Homo sapiens
5 AP2B1 163
Proximity Label-MS Homo sapiens
6 OR10H1  
Affinity Capture-MS Homo sapiens
7 STK25 10494
Affinity Capture-MS Homo sapiens
8 CLIP1 6249
Affinity Capture-MS Homo sapiens
9 PMS1  
Affinity Capture-Western Homo sapiens
10 ROPN1  
Affinity Capture-MS Homo sapiens
11 Csnk1e  
Two-hybrid Rattus norvegicus
12 SYCE1  
Affinity Capture-MS Homo sapiens
13 PPIE 10450
Affinity Capture-MS Homo sapiens
14 PKP2 5318
Two-hybrid Homo sapiens
15 SCNM1  
Two-hybrid Homo sapiens
16 FMR1 2332
Two-hybrid Homo sapiens
17 TPTE2  
Proximity Label-MS Homo sapiens
18 THOC2 57187
Affinity Capture-MS Homo sapiens
19 TSG101 7251
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
20 EFCAB1  
Affinity Capture-MS Homo sapiens
21 CALM1 801
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 CAMSAP2  
Proximity Label-MS Homo sapiens
23 GSK3A 2931
Affinity Capture-MS Homo sapiens
24 C1orf87  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PCNT  
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
26 IMPDH2 3615
Proximity Label-MS Homo sapiens
27 USO1 8615
Two-hybrid Homo sapiens
28 SSX5  
Two-hybrid Homo sapiens
29 NOLC1 9221
Affinity Capture-MS Homo sapiens
30 SOGA1 140710
Affinity Capture-MS Homo sapiens
31 WHAMMP3  
Affinity Capture-MS Homo sapiens
32 PRC1 9055
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 COQ3  
Affinity Capture-MS Homo sapiens
34 TNIK 23043
Two-hybrid Homo sapiens
35 Fgfr1op  
Affinity Capture-MS Mus musculus
36 TDP2 51567
Two-hybrid Homo sapiens
37 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NDUFAF1  
Affinity Capture-MS Homo sapiens
39 CEP57  
Affinity Capture-MS Homo sapiens
40 GTSE1 51512
Two-hybrid Homo sapiens
41 VDR  
Affinity Capture-MS Homo sapiens
42 CSNK1D 1453
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
43 CARD9 64170
Affinity Capture-MS Homo sapiens
44 SLU7  
Affinity Capture-MS Homo sapiens
45 RBM14 10432
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 KIF14 9928
Affinity Capture-MS Homo sapiens
47 CASK 8573
Two-hybrid Homo sapiens
48 RASSF2 9770
Proximity Label-MS Homo sapiens
49 MED1 5469
Two-hybrid Homo sapiens
50 PFN1 5216
Proximity Label-MS Homo sapiens
51 CALM2 805
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
52 KRT8 3856
Proximity Label-MS Homo sapiens
53 SAMD3  
Two-hybrid Homo sapiens
54 CCDC116  
Two-hybrid Homo sapiens
55 BEX2  
Two-hybrid Homo sapiens
56 TLX3  
Two-hybrid Homo sapiens
57 BNIP1 662
Affinity Capture-MS Homo sapiens
58 CTNNA3  
Two-hybrid Homo sapiens
59 CTBP1 1487
Two-hybrid Homo sapiens
60 GLIS2  
Two-hybrid Homo sapiens
61 KLC4 89953
Two-hybrid Homo sapiens
62 MAPRE1 22919
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 GOLGA2 2801
Proximity Label-MS Homo sapiens
64 SSX2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
65 RSRC2  
Two-hybrid Homo sapiens
66 RAMP1  
Affinity Capture-MS Homo sapiens
67 SLAIN1 122060
Two-hybrid Homo sapiens
68 FYN 2534
Affinity Capture-Western Homo sapiens
69 CEP170 9859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 TRIM21 6737
Affinity Capture-MS Homo sapiens
71 LINC00346  
Affinity Capture-MS Homo sapiens
72 TRIP10 9322
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
73 PKN1 5585
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
74 FNBP1 23048
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 STIL  
Proximity Label-MS Homo sapiens
76 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 Cdk5rap2  
Affinity Capture-MS Mus musculus
78 CEBPA  
Affinity Capture-MS Homo sapiens
79 PRKACB 5567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 FGFR1OP  
Affinity Capture-MS Homo sapiens
81 RBM10  
Two-hybrid Homo sapiens
82 YWHAE 7531
Two-hybrid Homo sapiens
83 DISC1 27185
Two-hybrid Homo sapiens
84 SF3B2 10992
Two-hybrid Homo sapiens
85 PRKACG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 GPC4 2239
Two-hybrid Homo sapiens
87 IPO5 3843
Affinity Capture-MS Homo sapiens
88 HDDC3  
Two-hybrid Homo sapiens
89 PXN 5829
Proximity Label-MS Homo sapiens
90 IKZF3  
Two-hybrid Homo sapiens
91 MTX2 10651
Proximity Label-MS Homo sapiens
92 SNRPB 6628
Two-hybrid Homo sapiens
93 AKAP9 10142
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
94 PRKAR2A 5576
Protein-peptide Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
95 SSX3  
Two-hybrid Homo sapiens
96 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
97 CUL3 8452
Affinity Capture-MS Homo sapiens
98 CCNE1  
Affinity Capture-Western Homo sapiens
99 MYO5A 4644
Two-hybrid Homo sapiens
100 FAM90A1  
Two-hybrid Homo sapiens
101 SNF8 11267
Two-hybrid Homo sapiens
102 SUZ12  
Affinity Capture-MS Homo sapiens
103 THAP1  
Two-hybrid Homo sapiens
104 SCYL2 55681
Affinity Capture-MS Homo sapiens
105 USHBP1  
Two-hybrid Homo sapiens
106 CDK5RAP2 55755
Proximity Label-MS Homo sapiens
107 MAPRE3  
Proximity Label-MS Homo sapiens
108 PRKACA 5566
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
109 CTCFL  
Affinity Capture-MS Homo sapiens
110 Uso1 56041
Affinity Capture-MS Mus musculus
111 FXR2 9513
Two-hybrid Homo sapiens
112 SUV39H2 79723
Two-hybrid Homo sapiens
113 C1orf94  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
114 NDC80 10403
Proximity Label-MS Homo sapiens
115 DNAJC10 54431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 PCGF1 84759
Affinity Capture-MS Homo sapiens
117 PIGF  
Affinity Capture-MS Homo sapiens
118 MAGEA6  
Two-hybrid Homo sapiens
119 RNF2  
Affinity Capture-MS Homo sapiens
120 BMI1  
Affinity Capture-MS Homo sapiens
121 PCM1 5108
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
122 STK4 6789
Affinity Capture-MS Homo sapiens
123 TCEANC  
Two-hybrid Homo sapiens
124 FAM46B  
Two-hybrid Homo sapiens
125 NANOG  
Affinity Capture-MS Homo sapiens
126 KRT19 3880
Proximity Label-MS Homo sapiens
127 Prkar2a 19087
Affinity Capture-MS Mus musculus
128 FTSJ3 117246
Affinity Capture-MS Homo sapiens
129 WAC  
Two-hybrid Homo sapiens
130 Prkacb 18749
Affinity Capture-MS Mus musculus
131 DDX55  
Affinity Capture-MS Homo sapiens
132 HUWE1 10075
Affinity Capture-MS Homo sapiens
133 ACBD5 91452
Proximity Label-MS Homo sapiens
134 TACC3  
Two-hybrid Homo sapiens
135 DVL2 1856
Affinity Capture-MS Homo sapiens
136 CLIC1 1192
Affinity Capture-Western Homo sapiens
137 CCDC68  
Affinity Capture-MS Homo sapiens
138 CEP170P1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 PRDM14  
Two-hybrid Homo sapiens
140 PARD6G 84552
Two-hybrid Homo sapiens
141 KIF19  
Two-hybrid Homo sapiens
142 FXR1 8087
Affinity Capture-MS Homo sapiens
143 BLZF1  
Two-hybrid Homo sapiens
144 BIRC6 57448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 ROPN1L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
146 HNRNPM 4670
Two-hybrid Homo sapiens
147 SEPT10 151011
Proximity Label-MS Homo sapiens
148 DCTN2 10540
Proximity Label-MS Homo sapiens
149 HDAC1 3065
Affinity Capture-MS Homo sapiens
150 KCNQ1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
151 LOC338797  
Affinity Capture-MS Homo sapiens
152 PRKAR2B 5577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
153 LATS1  
Proximity Label-MS Homo sapiens
154 BRK1 55845
Affinity Capture-MS Homo sapiens
155 TPM2 7169
Affinity Capture-MS Homo sapiens
156 CCDC146  
Two-hybrid Homo sapiens
157 ABI2 10152
Two-hybrid Homo sapiens
158 KDM1A 23028
Two-hybrid Homo sapiens
159 MAGED1 9500
Two-hybrid Homo sapiens
160 Mapre1 13589
Affinity Capture-MS Mus musculus
161 Prkar2b 19088
Affinity Capture-MS Mus musculus
162 CEP135  
Proximity Label-MS Homo sapiens
163 NDEL1 81565
Two-hybrid Homo sapiens
164 STK11 6794
Affinity Capture-MS Homo sapiens
165 RNF145  
Affinity Capture-MS Homo sapiens
166 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
167 POLR1D 51082
Affinity Capture-MS Homo sapiens
168 LMO1  
Two-hybrid Homo sapiens
169 ATOH1  
Affinity Capture-MS Homo sapiens
170 RPA2 6118
Two-hybrid Homo sapiens
171 RPA3 6119
Proximity Label-MS Homo sapiens
172 ZBTB44  
Affinity Capture-MS Homo sapiens
173 CDC5L 988
Two-hybrid Homo sapiens
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