Gene description for Prkacb
Gene name protein kinase, cAMP dependent, catalytic, beta
Gene symbol Prkacb
Other names/aliases Pkacb
Species Mus musculus
 Database cross references - Prkacb
ExoCarta ExoCarta_18749
Vesiclepedia VP_18749
Entrez Gene 18749
UniProt P68181  
 Prkacb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prkacb
Molecular Function
    magnesium ion binding GO:0000287 IDA
    magnesium ion binding GO:0000287 ISO
    protein serine/threonine kinase activity GO:0004674 TAS
    cAMP-dependent protein kinase activity GO:0004691 IBA
    cAMP-dependent protein kinase activity GO:0004691 IDA
    cAMP-dependent protein kinase activity GO:0004691 ISO
    ATP binding GO:0005524 IDA
    ATP binding GO:0005524 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein serine kinase activity GO:0106310 IEA
Biological Process
    neural tube closure GO:0001843 IGI
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    signal transduction GO:0007165 IBA
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 TAS
    negative regulation of smoothened signaling pathway GO:0045879 IGI
    negative regulation of meiotic cell cycle GO:0051447 ISO
    regulation of protein processing GO:0070613 IGI
    negative regulation of TORC1 signaling GO:1904262 ISO
    negative regulation of TORC1 signaling GO:1904262 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    cAMP-dependent protein kinase complex GO:0005952 IBA
    cAMP-dependent protein kinase complex GO:0005952 ISO
    cAMP-dependent protein kinase complex GO:0005952 TAS
    perinuclear region of cytoplasm GO:0048471 ISO
    ciliary base GO:0097546 IDA
 Experiment description of studies that identified Prkacb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prkacb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tnik  
Affinity Capture-MS Mus musculus
2 Cnksr2  
Affinity Capture-MS Mus musculus
3 Pde4dip  
Affinity Capture-MS Mus musculus
4 GOLGA3 2802
Affinity Capture-MS Homo sapiens
5 CEP131 22994
Affinity Capture-MS Homo sapiens
6 KIAA0753  
Affinity Capture-MS Homo sapiens
7 VAPB 9217
Affinity Capture-MS Homo sapiens
8 CANX 821
Affinity Capture-MS Homo sapiens
9 CEP68 23177
Affinity Capture-MS Homo sapiens
10 CEP170 9859
Affinity Capture-MS Homo sapiens
11 AKAP9 10142
Affinity Capture-MS Homo sapiens
12 CDK5RAP2 55755
Affinity Capture-MS Homo sapiens
13 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
14 Atxn1  
Affinity Capture-MS Mus musculus
15 PRKAR1A 5573
Affinity Capture-MS Homo sapiens
16 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
17 AKAP13 11214
Affinity Capture-MS Homo sapiens
18 CSPP1  
Affinity Capture-MS Homo sapiens
19 Iqcb1  
Affinity Capture-MS Mus musculus
20 Ywhaz 22631
Affinity Capture-MS Mus musculus
21 MAPRE1 22919
Affinity Capture-MS Homo sapiens
22 Tmem173  
Proximity Label-MS Mus musculus
23 OFD1 8481
Affinity Capture-MS Homo sapiens
24 PRKACA 5566
Affinity Capture-MS Homo sapiens
25 CEP72  
Affinity Capture-MS Homo sapiens
26 CCDC77  
Affinity Capture-MS Homo sapiens
27 PCNT  
Affinity Capture-MS Homo sapiens
28 Agap2  
Affinity Capture-MS Mus musculus
29 FGFR1OP2  
Affinity Capture-MS Homo sapiens
30 PKP4 8502
Affinity Capture-MS Homo sapiens
31 AKAP11 11215
Affinity Capture-MS Homo sapiens
32 TBC1D31  
Affinity Capture-MS Homo sapiens
33 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
34 PRKX 5613
Affinity Capture-MS Homo sapiens
35 Sstr3  
Proximity Label-MS Mus musculus
36 PCM1 5108
Affinity Capture-MS Homo sapiens
37 PRKAR1B  
Affinity Capture-MS Homo sapiens
38 Tia1  
Affinity Capture-MS Mus musculus
39 AKAP1 8165
Affinity Capture-MS Homo sapiens
40 FGFR1OP  
Affinity Capture-MS Homo sapiens
41 Fancd2  
Affinity Capture-MS Mus musculus
42 SCO2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Prkacb is involved
PathwayEvidenceSource
Activation of NMDA receptors and postsynaptic events IEA Reactome
Adaptive Immune System IEA Reactome
Aquaporin-mediated transport IEA Reactome
Axon guidance IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Ca-dependent events IEA Reactome
Calmodulin induced events IEA Reactome
CaM pathway IEA Reactome
CD209 (DC-SIGN) signaling IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
CREB1 phosphorylation through the activation of Adenylate Cyclase IEA Reactome
DAG and IP3 signaling IEA Reactome
DARPP-32 events IEA Reactome
Developmental Biology IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (s) signalling events IEA Reactome
G-protein mediated events IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
Glucagon signaling in metabolic regulation IEA Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
GPCR downstream signalling IEA Reactome
GPER1 signaling IEA Reactome
HDL assembly IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Integration of energy metabolism IEA Reactome
Intracellular signaling by second messengers IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Metabolism IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Opioid Signalling IEA Reactome
PKA activation IEA Reactome
PKA activation in glucagon signalling IEA Reactome
PKA-mediated phosphorylation of CREB IEA Reactome
Plasma lipoprotein assembly IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
PLC beta mediated events IEA Reactome
Post NMDA receptor activation events IEA Reactome
Rap1 signalling IEA Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
RET signaling IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by VEGF IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome





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