Gene description for Prkar2a
Gene name protein kinase, cAMP dependent regulatory, type II alpha
Gene symbol Prkar2a
Other names/aliases 1110061A24Rik
AI317181
AI836829
RII(alpha)
Species Mus musculus
 Database cross references - Prkar2a
ExoCarta ExoCarta_19087
Vesiclepedia VP_19087
Entrez Gene 19087
 Prkar2a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prkar2a
Molecular Function
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IBA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    protein binding GO:0005515 IPI
    cAMP-dependent protein kinase regulator activity GO:0008603 IEA
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
    protein domain specific binding GO:0019904 ISO
    cAMP binding GO:0030552 IBA
    cAMP binding GO:0030552 IEA
    cAMP binding GO:0030552 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    beta-2 adrenergic receptor binding GO:0031698 ISO
    protein kinase A catalytic subunit binding GO:0034236 IBA
    protein kinase A catalytic subunit binding GO:0034236 ISO
    small molecule binding GO:0036094 ISO
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    negative regulation of cAMP/PKA signal transduction GO:0141162 ISO
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    axoneme GO:0005930 ISO
    cAMP-dependent protein kinase complex GO:0005952 IBA
    cAMP-dependent protein kinase complex GO:0005952 IDA
    cAMP-dependent protein kinase complex GO:0005952 ISO
    T-tubule GO:0030315 ISO
    nucleotide-activated protein kinase complex GO:0031588 ISO
    protein-containing complex GO:0032991 ISO
    plasma membrane raft GO:0044853 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Prkar2a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prkar2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tnik  
Affinity Capture-MS Mus musculus
2 Gskip  
Co-fractionation Mus musculus
3 Pde4dip  
Affinity Capture-MS Mus musculus
4 GOLGA3 2802
Affinity Capture-MS Homo sapiens
5 CEP131 22994
Affinity Capture-MS Homo sapiens
6 HADHB 3032
Affinity Capture-MS Homo sapiens
7 KIAA0753  
Affinity Capture-MS Homo sapiens
8 CEP68 23177
Affinity Capture-MS Homo sapiens
9 WDR90  
Affinity Capture-MS Homo sapiens
10 CRYBG3  
Affinity Capture-MS Homo sapiens
11 CEP170 9859
Affinity Capture-MS Homo sapiens
12 AKAP9 10142
Affinity Capture-MS Homo sapiens
13 Syngap1  
Affinity Capture-MS Mus musculus
14 CEP72  
Affinity Capture-MS Homo sapiens
15 PRKAR1A 5573
Affinity Capture-MS Homo sapiens
16 PLIN4  
Affinity Capture-MS Homo sapiens
17 Kctd13  
Affinity Capture-MS Mus musculus
18 MIB1 57534
Affinity Capture-MS Homo sapiens
19 AKAP13 11214
Affinity Capture-MS Homo sapiens
20 PIBF1  
Affinity Capture-MS Homo sapiens
21 Ywhaz 22631
Affinity Capture-MS Mus musculus
22 CEP295  
Affinity Capture-MS Homo sapiens
23 CEP78 84131
Affinity Capture-MS Homo sapiens
24 MAPRE1 22919
Affinity Capture-MS Homo sapiens
25 CSPP1  
Affinity Capture-MS Homo sapiens
26 CDK5RAP2 55755
Affinity Capture-MS Homo sapiens
27 OFD1 8481
Affinity Capture-MS Homo sapiens
28 PRKACA 5566
Affinity Capture-MS Homo sapiens
29 CCDC77  
Affinity Capture-MS Homo sapiens
30 PCNT  
Affinity Capture-MS Homo sapiens
31 CEP57L1  
Affinity Capture-MS Homo sapiens
32 FARP1 10160
Affinity Capture-MS Homo sapiens
33 CCDC14  
Affinity Capture-MS Homo sapiens
34 TBC1D31  
Affinity Capture-MS Homo sapiens
35 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
36 AKAP2 11217
Affinity Capture-MS Homo sapiens
37 PCM1 5108
Affinity Capture-MS Homo sapiens
38 CEP350 9857
Affinity Capture-MS Homo sapiens
39 AKAP1 8165
Affinity Capture-MS Homo sapiens
40 PRKACB 5567
Affinity Capture-MS Homo sapiens
41 FGFR1OP  
Affinity Capture-MS Homo sapiens
42 CCDC61  
Affinity Capture-MS Homo sapiens
43 SSX2IP  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Prkar2a is involved
No pathways found





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