Gene description for MIB2
Gene name mindbomb E3 ubiquitin protein ligase 2
Gene symbol MIB2
Other names/aliases ZZANK1
ZZZ5
Species Homo sapiens
 Database cross references - MIB2
ExoCarta ExoCarta_142678
Vesiclepedia VP_142678
Entrez Gene 142678
HGNC 30577
MIM 611141
UniProt Q96AX9  
 MIB2 identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for MIB2
Molecular Function
    actin binding GO:0003779 IEA
    ubiquitin-protein transferase activity GO:0004842 EXP
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    Notch signaling pathway GO:0007219 IEA
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 TAS
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IEA
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified MIB2 in sEVs
1
Experiment ID 274
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for MIB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 DEFB125  
Affinity Capture-MS Homo sapiens
3 KCNH2  
Affinity Capture-MS Homo sapiens
4 TRIP6 7205
Affinity Capture-MS Homo sapiens
5 C6orf15  
Affinity Capture-MS Homo sapiens
6 MAVS 57506
Affinity Capture-MS Homo sapiens
7 UBE2N 7334
Reconstituted Complex Homo sapiens
8 LPPR4  
Affinity Capture-MS Homo sapiens
9 CCT3 7203
Affinity Capture-MS Homo sapiens
10 CFLAR  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 CUL3 8452
Affinity Capture-MS Homo sapiens
12 TNF  
Affinity Capture-Western Homo sapiens
13 CCT2 10576
Affinity Capture-MS Homo sapiens
14 PMCH  
Affinity Capture-MS Homo sapiens
15 LYPD6B 130576
Affinity Capture-MS Homo sapiens
16 NUPL1 9818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PI15  
Affinity Capture-MS Homo sapiens
18 Bcl10  
Affinity Capture-Western Mus musculus
19 LOXL4 84171
Affinity Capture-MS Homo sapiens
20 BCL10  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 SERINC5 256987
Affinity Capture-MS Homo sapiens
22 IKZF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NAF1  
Affinity Capture-MS Homo sapiens
24 CARD6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 CCT7 10574
Affinity Capture-MS Homo sapiens
26 DMTN  
Affinity Capture-MS Homo sapiens
27 CCT5 22948
Affinity Capture-MS Homo sapiens
28 APEX1 328
Affinity Capture-RNA Homo sapiens
29 TBK1 29110
Biochemical Activity Homo sapiens
30 UBE2D2 7322
Reconstituted Complex Homo sapiens
31 ACTA1 58
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
32 IST1 9798
Co-fractionation Homo sapiens
33 CHRNB4  
Affinity Capture-MS Homo sapiens
34 GRIN2B  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
35 MACC1 346389
Affinity Capture-MS Homo sapiens
36 P2RY1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PLA2G16 11145
Affinity Capture-MS Homo sapiens
38 UBE2U  
Two-hybrid Homo sapiens
39 JAG2  
Reconstituted Complex Homo sapiens
40 RIPK1 8737
Affinity Capture-Western Homo sapiens
41 TMEM74  
Affinity Capture-MS Homo sapiens
42 F9 2158
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ELANE 1991
Affinity Capture-MS Homo sapiens
44 BAGE2  
Affinity Capture-MS Homo sapiens
45 MAP3K7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 SRP14 6727
Affinity Capture-MS Homo sapiens
47 IKZF5 64376
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CYLD  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
49 XRCC5 7520
Affinity Capture-MS Homo sapiens
50 IKBKG 8517
Affinity Capture-Western Homo sapiens
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