Gene description for Grb2
Gene name growth factor receptor bound protein 2
Gene symbol Grb2
Other names/aliases AA408164
Ash
Species Mus musculus
 Database cross references - Grb2
ExoCarta ExoCarta_14784
Vesiclepedia VP_14784
Entrez Gene 14784
UniProt Q60631  
 Grb2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Grb2
Molecular Function
    phosphotyrosine residue binding GO:0001784 IBA
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 IGI
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 ISO
    guanyl-nucleotide exchange factor adaptor activity GO:0005091 IDA
    guanyl-nucleotide exchange factor adaptor activity GO:0005091 ISO
    epidermal growth factor receptor binding GO:0005154 IBA
    epidermal growth factor receptor binding GO:0005154 IEA
    epidermal growth factor receptor binding GO:0005154 ISO
    neurotrophin TRKA receptor binding GO:0005168 IEA
    neurotrophin TRKA receptor binding GO:0005168 ISO
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 ISO
    SH3 domain binding GO:0017124 ISS
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 ISO
    protein phosphatase binding GO:0019903 IPI
    protein domain specific binding GO:0019904 IPI
    protein domain specific binding GO:0019904 ISO
    protein-macromolecule adaptor activity GO:0030674 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    insulin receptor substrate binding GO:0043560 IEA
    insulin receptor substrate binding GO:0043560 ISO
    protein-containing complex binding GO:0044877 ISO
    ephrin receptor binding GO:0046875 IPI
    ephrin receptor binding GO:0046875 ISO
    phosphoprotein binding GO:0051219 ISO
    molecular adaptor activity GO:0060090 ISO
Biological Process
    signal transduction GO:0007165 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IGI
    epidermal growth factor receptor signaling pathway GO:0007173 ISO
    Ras protein signal transduction GO:0007265 TAS
    insulin receptor signaling pathway GO:0008286 IDA
    insulin receptor signaling pathway GO:0008286 ISO
    fibroblast growth factor receptor signaling pathway GO:0008543 IGI
    Schwann cell development GO:0014044 IMP
    actin cytoskeleton organization GO:0030036 IEA
    actin cytoskeleton organization GO:0030036 ISO
    positive regulation of actin filament polymerization GO:0030838 IGI
    receptor internalization GO:0031623 IEA
    receptor internalization GO:0031623 ISO
    endodermal cell differentiation GO:0035987 IMP
    ERBB2-ERBB3 signaling pathway GO:0038133 ISO
    T cell activation GO:0042110 IEA
    natural killer cell mediated cytotoxicity GO:0042267 IEA
    natural killer cell mediated cytotoxicity GO:0042267 ISO
    myelination GO:0042552 IMP
    signal transduction in response to DNA damage GO:0042770 IEA
    signal transduction in response to DNA damage GO:0042770 ISO
    regulation of MAPK cascade GO:0043408 IBA
    regulation of MAPK cascade GO:0043408 IGI
    insulin-like growth factor receptor signaling pathway GO:0048009 IDA
    anatomical structure formation involved in morphogenesis GO:0048646 IMP
    B cell receptor signaling pathway GO:0050853 IDA
    branching involved in labyrinthine layer morphogenesis GO:0060670 IMP
    cellular response to ionizing radiation GO:0071479 IEA
    cellular response to ionizing radiation GO:0071479 ISO
    positive regulation of reactive oxygen species metabolic process GO:2000379 IEA
    positive regulation of reactive oxygen species metabolic process GO:2000379 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 ISO
    endosome GO:0005768 ISS
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell-cell junction GO:0005911 IDA
    cell cortex GO:0005938 IGI
    COP9 signalosome GO:0008180 IBA
    COP9 signalosome GO:0008180 IDA
    COP9 signalosome GO:0008180 ISO
    vesicle membrane GO:0012506 IDA
    membrane GO:0016020 IDA
    protein-containing complex GO:0032991 ISO
    Grb2-EGFR complex GO:0070436 IEA
    Grb2-EGFR complex GO:0070436 ISO
 Experiment description of studies that identified Grb2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Grb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nufip2  
Two-hybrid Mus musculus
2 Gab2  
Affinity Capture-MS Mus musculus
3 Csf1r  
Co-fractionation Mus musculus
4 CD28  
Affinity Capture-Western Homo sapiens
5 Pdgfrb 18596
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
6 EXOSC7 23016
Two-hybrid Homo sapiens
7 Sh3kbp1 58194
Co-fractionation Mus musculus
8 Sf3b2  
Two-hybrid Mus musculus
9 Cpsf6  
Two-hybrid Mus musculus
10 Prnp 19122
Affinity Capture-Western Mus musculus
11 Lrrk1  
Affinity Capture-MS Mus musculus
12 Met 17295
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
13 Cpne2  
Affinity Capture-MS Mus musculus
14 Zfp106  
Affinity Capture-Western Mus musculus
15 Pik3r1  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
16 YTHDF1 54915
Two-hybrid Homo sapiens
17 Cstf2t  
Two-hybrid Mus musculus
18 RBMX 27316
Two-hybrid Homo sapiens
19 Ptpn18  
Co-fractionation Mus musculus
20 SF3B4 10262
Two-hybrid Homo sapiens
21 Ybx1 22608
Affinity Capture-MS Mus musculus
22 DMRTB1  
Two-hybrid Homo sapiens
23 Dvl2  
Affinity Capture-Western Mus musculus
24 Kctd13  
Affinity Capture-MS Mus musculus
25 Rbm14  
Two-hybrid Mus musculus
26 Ptpn23 104831
Co-fractionation Mus musculus
27 Kit  
Two-hybrid Mus musculus
28 Axl 26362
Affinity Capture-Western Mus musculus
29 Shc1  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
30 Dok3  
Affinity Capture-Western Mus musculus
31 Frs2  
Affinity Capture-Western Mus musculus
32 SNRPA 6626
Two-hybrid Homo sapiens
33 PRRC2B  
Two-hybrid Homo sapiens
34 APP 351
Affinity Capture-Western Homo sapiens
35 Zc3h7b  
Two-hybrid Mus musculus
36 Gab1  
Affinity Capture-Western Mus musculus
37 Krt36  
Co-fractionation Mus musculus
38 Kdr  
Affinity Capture-Western Mus musculus
39 Dok1  
Affinity Capture-MS Mus musculus
40 Spry2  
Affinity Capture-Western Mus musculus
41 Fam175b  
Co-fractionation Mus musculus
42 Spry1  
Affinity Capture-Western Mus musculus
43 FRS2 10818
Affinity Capture-Western Homo sapiens
44 Tek  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
45 Bcr  
Affinity Capture-MS Mus musculus
46 Ralgps2  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
47 SNRPN 6638
Two-hybrid Homo sapiens
48 Nf2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
49 CPSF7 79869
Two-hybrid Homo sapiens
50 Sh2b2  
Affinity Capture-Western Mus musculus
51 Hnrnpk 15387
Two-hybrid Mus musculus
52 Fip1l1  
Two-hybrid Mus musculus
53 Dynlt1b  
Two-hybrid Mus musculus
54 DDX17 10521
Two-hybrid Homo sapiens
55 Syp  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
56 TFIP11  
Two-hybrid Homo sapiens
57 Egfr 13649
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
58 Nudt21 68219
Two-hybrid Mus musculus
59 Ube2i  
Two-hybrid Mus musculus
60 Pspc1  
Two-hybrid Mus musculus
61 Atg16l1  
Affinity Capture-MS Mus musculus
62 Sos1  
Co-fractionation Mus musculus
63 4732456N10Rik 239673
Affinity Capture-MS Mus musculus
64 Cbl 12402
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
65 Fancd2  
Affinity Capture-MS Mus musculus
66 Bre  
Co-fractionation Mus musculus
67 EXOSC8  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Grb2 is involved
PathwayEvidenceSource
Activated NTRK2 signals through FRS2 and FRS3 IEA Reactome
Adaptive Immune System IEA Reactome
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers IEA Reactome
Axon guidance IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
CD28 co-stimulation IEA Reactome
CD28 dependent Vav1 pathway IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cell-Cell communication IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Costimulation by the CD28 family IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DAP12 interactions IEA Reactome
DAP12 signaling IEA Reactome
Developmental Biology IEA Reactome
Downstream signal transduction IEA Reactome
Downstream signaling of activated FGFR1 IEA Reactome
Downstream signaling of activated FGFR2 IEA Reactome
Downstream signaling of activated FGFR3 IEA Reactome
Downstream signaling of activated FGFR4 IEA Reactome
EGFR downregulation IEA Reactome
EGFR Transactivation by Gastrin IEA Reactome
Erythropoietin activates RAS IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated Ca+2 mobilization IEA Reactome
FCERI mediated MAPK activation IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
FLT3 Signaling IEA Reactome
FRS-mediated FGFR1 signaling IEA Reactome
FRS-mediated FGFR2 signaling IEA Reactome
FRS-mediated FGFR3 signaling IEA Reactome
FRS-mediated FGFR4 signaling IEA Reactome
G alpha (q) signalling events IEA Reactome
GAB1 signalosome IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
GPCR downstream signalling IEA Reactome
GRB2 events in EGFR signaling IEA Reactome
GRB2 events in ERBB2 signaling IEA Reactome
GRB2:SOS provides linkage to MAPK signaling for Integrins IEA Reactome
Hemostasis IEA Reactome
IGF1R signaling cascade IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Insulin receptor signalling cascade IEA Reactome
Integrin signaling IEA Reactome
Interleukin receptor SHC signaling IEA Reactome
Interleukin-15 signaling IEA Reactome
Interleukin-2 family signaling IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
IRS-mediated signalling IEA Reactome
IRS-related events triggered by IGF1R IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Membrane Trafficking IEA Reactome
MET activates PI3K/AKT signaling IEA Reactome
MET activates PTPN11 IEA Reactome
MET activates RAP1 and RAC1 IEA Reactome
MET activates RAS signaling IEA Reactome
MET promotes cell motility IEA Reactome
MET receptor recycling IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of MET activity IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
Nervous system development IEA Reactome
PI-3K cascade:FGFR1 IEA Reactome
PI-3K cascade:FGFR2 IEA Reactome
PI-3K cascade:FGFR3 IEA Reactome
PI-3K cascade:FGFR4 IEA Reactome
PI3K Cascade IEA Reactome
PI3K events in ERBB2 signaling IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet Aggregation (Plug Formation) IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
Regulation of KIT signaling IEA Reactome
Regulation of signaling by CBL IEA Reactome
RET signaling IEA Reactome
RHO GTPase cycle IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RHOU GTPase cycle IEA Reactome
Role of LAT2/NTAL/LAB on calcium mobilization IEA Reactome
SHC-mediated cascade:FGFR1 IEA Reactome
SHC-mediated cascade:FGFR2 IEA Reactome
SHC-mediated cascade:FGFR3 IEA Reactome
SHC-mediated cascade:FGFR4 IEA Reactome
SHC-related events triggered by IGF1R IEA Reactome
SHC1 events in EGFR signaling IEA Reactome
SHC1 events in ERBB4 signaling IEA Reactome
Signal attenuation IEA Reactome
Signal regulatory protein family interactions IEA Reactome
Signal Transduction IEA Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by ERBB2 IEA Reactome
Signaling by ERBB4 IEA Reactome
Signaling by Erythropoietin IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Insulin receptor IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by LTK IEA Reactome
Signaling by MET IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRK2 (TRKB) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by PDGF IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) IEA Reactome
Signalling to ERKs IEA Reactome
Signalling to RAS IEA Reactome
SOS-mediated signalling IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Tie2 Signaling IEA Reactome
Vesicle-mediated transport IEA Reactome





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