Gene description for Gstm1
Gene name glutathione S-transferase, mu 1
Gene symbol Gstm1
Other names/aliases Gstb-1
Gstb1
Species Mus musculus
 Database cross references - Gstm1
ExoCarta ExoCarta_14862
Vesiclepedia VP_14862
Entrez Gene 14862
UniProt P10649  
 Gstm1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gstm1
Molecular Function
    glutathione transferase activity GO:0004364 IBA
    glutathione transferase activity GO:0004364 ISO
    glutathione transferase activity GO:0004364 ISS
    steroid binding GO:0005496 ISO
    protein binding GO:0005515 IPI
    nickel cation binding GO:0016151 ISO
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    glutathione binding GO:0043295 ISO
Biological Process
    prostaglandin metabolic process GO:0006693 ISS
    glutathione metabolic process GO:0006749 IBA
    glutathione metabolic process GO:0006749 ISO
    xenobiotic catabolic process GO:0042178 ISO
    hepoxilin biosynthetic process GO:0051122 ISS
    cellular response to xenobiotic stimulus GO:0071466 IDA
    glutathione derivative biosynthetic process GO:1901687 ISS
Subcellular Localization
    extracellular region GO:0005576 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    protein-containing complex GO:0032991 ISO
    myelin sheath GO:0043209 HDA
    intercellular bridge GO:0045171 ISO
 Experiment description of studies that identified Gstm1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gstm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sstr3  
Proximity Label-MS Mus musculus
2 MAP3K5 4217
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 Kctd13  
Affinity Capture-MS Mus musculus
4 MAPT  
Reconstituted Complex Homo sapiens
5 Tia1  
Affinity Capture-MS Mus musculus
6 HSD17B10 3028
Affinity Capture-MS Homo sapiens
7 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Gstm1 is involved
PathwayEvidenceSource
Biological oxidations IEA Reactome
Glutathione conjugation IEA Reactome
Metabolism IEA Reactome
Phase II - Conjugation of compounds IEA Reactome





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