Gene description for MAP3K5
Gene name mitogen-activated protein kinase kinase kinase 5
Gene symbol MAP3K5
Other names/aliases ASK1
MAPKKK5
MEKK5
Species Homo sapiens
 Database cross references - MAP3K5
ExoCarta ExoCarta_4217
Vesiclepedia VP_4217
Entrez Gene 4217
HGNC 6857
MIM 602448
UniProt Q99683  
 MAP3K5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAP3K5
Molecular Function
    magnesium ion binding GO:0000287 IDA
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    JUN kinase kinase kinase activity GO:0004706 IDA
    MAP kinase kinase kinase activity GO:0004709 IBA
    MAP kinase kinase kinase activity GO:0004709 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    protein kinase binding GO:0019901 IEA
    protein phosphatase binding GO:0019903 IPI
    protein domain specific binding GO:0019904 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    MAPK cascade GO:0000165 IDA
    response to ischemia GO:0002931 IEA
    protein phosphorylation GO:0006468 IDA
    JNK cascade GO:0007254 IBA
    JNK cascade GO:0007254 IDA
    JNK cascade GO:0007254 IMP
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IBA
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IDA
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 IEA
    cellular response to amino acid starvation GO:0034198 IDA
    response to endoplasmic reticulum stress GO:0034976 IMP
    neuron intrinsic apoptotic signaling pathway in response to oxidative stress GO:0036480 IGI
    p38MAPK cascade GO:0038066 IBA
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of JUN kinase activity GO:0043507 IMP
    innate immune response GO:0045087 IEA
    positive regulation of myoblast differentiation GO:0045663 IEA
    positive regulation of protein kinase activity GO:0045860 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of JNK cascade GO:0046330 ISS
    neuron apoptotic process GO:0051402 IBA
    neuron apoptotic process GO:0051402 ISS
    stress-activated MAPK cascade GO:0051403 IDA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 IBA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 ISS
    cellular response to hydrogen peroxide GO:0070301 IDA
    cellular response to tumor necrosis factor GO:0071356 IEA
    endothelial cell apoptotic process GO:0072577 IEA
    cellular senescence GO:0090398 TAS
    apoptotic signaling pathway GO:0097190 IDA
    programmed necrotic cell death GO:0097300 IEA
    positive regulation of p38MAPK cascade GO:1900745 IEA
    cellular response to reactive nitrogen species GO:1902170 IEA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    external side of plasma membrane GO:0009897 IEA
    protein-containing complex GO:0032991 IMP
    protein kinase complex GO:1902911 IDA
    IRE1-TRAF2-ASK1 complex GO:1990604 IDA
 Experiment description of studies that identified MAP3K5 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAP3K5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
2 MCRS1  
Affinity Capture-Western Homo sapiens
3 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 GNA12 2768
Affinity Capture-Western Homo sapiens
5 ERN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
6 QARS 5859
Affinity Capture-Western Homo sapiens
7 TRAF4 9618
Affinity Capture-Western Homo sapiens
8 RABEP2 79874
Affinity Capture-MS Homo sapiens
9 YWHAE 7531
Affinity Capture-MS Homo sapiens
10 USP9X 8239
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
11 SOCS1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 PARK7 11315
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 APP 351
Co-localization Homo sapiens
14 CDKN1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
15 Rb1cc1  
Affinity Capture-Western Mus musculus
16 MAP3K5 4217
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
17 DYRK1A 1859
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
19 GLRX 2745
Affinity Capture-Western Homo sapiens
20 YWHAG 7532
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 DMD 1756
Two-hybrid Homo sapiens
22 PSMC2 5701
Affinity Capture-Western Homo sapiens
23 ALK 238
Affinity Capture-MS Homo sapiens
24 YWHAH 7533
Affinity Capture-MS Homo sapiens
25 MAP3K6  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
26 Mbp 17196
Biochemical Activity Mus musculus
27 SOCS3 9021
Affinity Capture-Western Homo sapiens
28 MAPK4  
Affinity Capture-MS Homo sapiens
29 NEDD4 4734
Biochemical Activity Homo sapiens
30 PSMC3 5702
Affinity Capture-Western Homo sapiens
31 FAS 355
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
32 YWHAB 7529
Affinity Capture-MS Homo sapiens
33 EIF2AK2 5610
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 PPP5C 5536
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
35 CUL3 8452
Affinity Capture-MS Homo sapiens
36 RB1CC1 9821
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 PSMC1 5700
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
38 UBE2I 7329
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
39 MBP 4155
Biochemical Activity Homo sapiens
40 YWHAQ 10971
Reconstituted Complex Homo sapiens
41 APOBEC3G  
Affinity Capture-Western Homo sapiens
42 GNA13 10672
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
43 DAXX  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
44 UBXN6 80700
Affinity Capture-MS Homo sapiens
45 MAP2K7 5609
Affinity Capture-Western Homo sapiens
46 TXN2 25828
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
47 PTPN11 5781
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
48 Gstm1 14862
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
49 TNFAIP8L1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 KMT2A  
Affinity Capture-Western Homo sapiens
51 TRAF7  
Affinity Capture-Western Homo sapiens
52 PRKAA2 5563
Affinity Capture-MS Homo sapiens
53 RNF150  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
54 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 GADD45B  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
56 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
57 METTL21B  
Affinity Capture-MS Homo sapiens
58 MAP3K7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 TRAF3 7187
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 CDC25C  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
61 TRAF5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 NACC1 112939
Affinity Capture-MS Homo sapiens
63 HSPA1A 3303
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
64 HSPA4 3308
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 PSMC5 5705
Affinity Capture-Western Homo sapiens
66 RAF1 5894
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
67 PRKAB2 5565
Affinity Capture-MS Homo sapiens
68 TNFSF13B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 BRD3 8019
Affinity Capture-MS Homo sapiens
70 NEDD4L 23327
Biochemical Activity Homo sapiens
71 SUMO1 7341
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
72 STUB1 10273
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
73 DUSP19  
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
74 DAB2IP 153090
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
75 CDC25A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
76 ARRB2 409
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
77 MAP2K6 5608
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
78 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
79 SMN1 6606
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 MAP2K4 6416
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
81 PDCD6 10016
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
82 CREBBP  
Two-hybrid Homo sapiens
83 OTUB1 55611
Affinity Capture-MS Homo sapiens
84 ATAD3A 55210
Affinity Capture-MS Homo sapiens
85 TXN 7295
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
86 GAPDH 2597
Affinity Capture-Western Homo sapiens
87 ARRB1 408
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
88 MAPK8IP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
89 GSTM1 2944
Affinity Capture-Western Homo sapiens
90 HSF2BP  
Affinity Capture-MS Homo sapiens
91 PSMC4 5704
Affinity Capture-Western Homo sapiens
92 JAK2 3717
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
93 PRMT1 3276
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
94 EP300 2033
Two-hybrid Homo sapiens
95 TRAF1 7185
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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