Gene description for MAP2K6
Gene name mitogen-activated protein kinase kinase 6
Gene symbol MAP2K6
Other names/aliases MAPKK6
MEK6
MKK6
PRKMK6
SAPKK-3
SAPKK3
Species Homo sapiens
 Database cross references - MAP2K6
ExoCarta ExoCarta_5608
Vesiclepedia VP_5608
Entrez Gene 5608
HGNC 6846
MIM 601254
UniProt P52564  
 MAP2K6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
 Gene ontology annotations for MAP2K6
Molecular Function
    protein serine/threonine kinase activity GO:0004674 TAS
    MAP kinase kinase activity GO:0004708 IBA
    MAP kinase kinase activity GO:0004708 IDA
    MAP kinase kinase activity GO:0004708 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    JUN kinase kinase activity GO:0008545 IEA
    protein kinase binding GO:0019901 IPI
    histone H3Y41 kinase activity GO:0035401 IEA
    identical protein binding GO:0042802 IPI
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    MAPK cascade GO:0000165 IDA
    osteoblast differentiation GO:0001649 IEA
    response to ischemia GO:0002931 IEA
    chromatin remodeling GO:0006338 IEA
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 TAS
    JNK cascade GO:0007254 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    ovulation cycle process GO:0022602 IEA
    stress-activated protein kinase signaling cascade GO:0031098 IDA
    positive regulation of prostaglandin secretion GO:0032308 IEA
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    p38MAPK cascade GO:0038066 IDA
    p38MAPK cascade GO:0038066 TAS
    signal transduction in response to DNA damage GO:0042770 TAS
    positive regulation of apoptotic process GO:0043065 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    stress-activated MAPK cascade GO:0051403 IDA
    regulation of cell cycle GO:0051726 TAS
    cardiac muscle contraction GO:0060048 IEA
    bone development GO:0060348 IEA
    cellular response to sorbitol GO:0072709 IEA
    cellular senescence GO:0090398 TAS
    negative regulation of cold-induced thermogenesis GO:0120163 ISS
    regulation of signal transduction by p53 class mediator GO:1901796 TAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified MAP2K6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAP2K6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA 5970
Co-localization Homo sapiens
2 MAPKAPK2 9261
Biochemical Activity Homo sapiens
3 CST1  
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 TRAF6 7189
Affinity Capture-Western Homo sapiens
6 CDCA4 55038
Affinity Capture-MS Homo sapiens
7 MAP2K4 6416
Biochemical Activity Homo sapiens
8 SMAD7  
Affinity Capture-Western Homo sapiens
9 PLCB2  
Affinity Capture-Western Homo sapiens
10 MAPK1 5594
Biochemical Activity Homo sapiens
11 GADD45A  
Co-localization Homo sapiens
12 C1QA 712
Affinity Capture-MS Homo sapiens
13 CDC37 11140
Affinity Capture-MS Homo sapiens
14 Mapk14  
Affinity Capture-Western Mus musculus
15 PTEN 5728
Affinity Capture-MS Homo sapiens
16 MAPK12  
Biochemical Activity Homo sapiens
17 MLH1 4292
Two-hybrid Homo sapiens
18 NPHS1 4868
Phenotypic Suppression Homo sapiens
19 MAP3K7  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
20 MAP3K5 4217
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
21 MAP2K3 5606
Phenotypic Enhancement Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TAOK2 9344
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
23 LRRK2 120892
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
24 TLR3  
Biochemical Activity Homo sapiens
25 ZG16B 124220
Affinity Capture-MS Homo sapiens
26 MAP3K4 4216
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
27 DUSP4 1846
Affinity Capture-MS Homo sapiens
28 PKN1 5585
Affinity Capture-Western Homo sapiens
29 GNB2L1 10399
Affinity Capture-Western Homo sapiens
30 MAPK14 1432
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
31 LMNA 4000
Proximity Label-MS Homo sapiens
32 FBXO31 79791
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MAP2K6 is involved
PathwayEvidenceSource
activated TAK1 mediates p38 MAPK activation TAS Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Bacterial Infection Pathways TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Interferon Signaling TAS Reactome
Interleukin-1 family signaling TAS Reactome
Interleukin-1 signaling TAS Reactome
Interleukin-17 signaling TAS Reactome
MAP kinase activation TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Myogenesis IEA Reactome
NOD1/2 Signaling Pathway TAS Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
PI5P Regulates TP53 Acetylation TAS Reactome
PKR-mediated signaling TAS Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Activity through Acetylation TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Signaling by Interleukins TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome
Uptake and actions of bacterial toxins TAS Reactome
Uptake and function of anthrax toxins TAS Reactome





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