Gene description for MAPKAPK2
Gene name mitogen-activated protein kinase-activated protein kinase 2
Gene symbol MAPKAPK2
Other names/aliases MAPKAP-K2
MK-2
MK2
Species Homo sapiens
 Database cross references - MAPKAPK2
ExoCarta ExoCarta_9261
Vesiclepedia VP_9261
Entrez Gene 9261
HGNC 6887
MIM 602006
UniProt P49137  
 MAPKAPK2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MAPKAPK2
Molecular Function
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 EXP
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    calcium/calmodulin-dependent protein kinase activity GO:0004683 IBA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    ATP binding GO:0005524 IEA
    calcium-dependent protein serine/threonine kinase activity GO:0009931 IBA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    mitogen-activated protein kinase binding GO:0051019 IBA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    MAPK cascade GO:0000165 TAS
    toll-like receptor signaling pathway GO:0002224 IBA
    toll-like receptor signaling pathway GO:0002224 ISS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    leukotriene metabolic process GO:0006691 TAS
    inflammatory response GO:0006954 IBA
    inflammatory response GO:0006954 ISS
    DNA damage response GO:0006974 IMP
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    response to lipopolysaccharide GO:0032496 ISS
    regulation of interleukin-6 production GO:0032675 ISS
    regulation of tumor necrosis factor production GO:0032680 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    response to cytokine GO:0034097 IBA
    response to cytokine GO:0034097 IDA
    intracellular signal transduction GO:0035556 IBA
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IBA
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IMP
    p38MAPK cascade GO:0038066 IEA
    regulation of mRNA stability GO:0043488 TAS
    macropinocytosis GO:0044351 ISS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IBA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    inner ear development GO:0048839 IEA
    positive regulation of macrophage cytokine production GO:0060907 IEA
    3'-UTR-mediated mRNA stabilization GO:0070935 IDA
    regulation of cellular response to heat GO:1900034 TAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MAPKAPK2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAPKAPK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPATS2  
Affinity Capture-MS Homo sapiens
2 LSP1 4046
Biochemical Activity Homo sapiens
3 Mapk14  
Biochemical Activity Mus musculus
Reconstituted Complex Mus musculus
4 SHC1 6464
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
5 DHX8 1659
Affinity Capture-MS Homo sapiens
6 CPSF4  
Affinity Capture-MS Homo sapiens
7 ZC3H4 23211
Affinity Capture-MS Homo sapiens
8 BPNT1 10380
Co-fractionation Homo sapiens
9 PRPF40A 55660
Affinity Capture-MS Homo sapiens
10 TTPAL 79183
Affinity Capture-MS Homo sapiens
11 SAFB 6294
Affinity Capture-MS Homo sapiens
12 TCF3  
Reconstituted Complex Homo sapiens
13 TRIM28 10155
Biochemical Activity Homo sapiens
14 UTP14A 10813
Affinity Capture-MS Homo sapiens
15 KIF23 9493
Affinity Capture-MS Homo sapiens
16 CDK11B 984
Affinity Capture-MS Homo sapiens
17 ZNF638 27332
Affinity Capture-MS Homo sapiens
18 PRKY  
Affinity Capture-MS Homo sapiens
19 COPS5 10987
Affinity Capture-MS Homo sapiens
20 CDK12 51755
Affinity Capture-MS Homo sapiens
21 MAPK14 1432
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
22 Ube2j1  
Affinity Capture-Western Mus musculus
23 Zfp36  
Biochemical Activity Mus musculus
24 DNAJB1 3337
Co-fractionation Homo sapiens
25 MTHFD2L 441024
Affinity Capture-MS Homo sapiens
26 ECHDC1 55862
Co-fractionation Homo sapiens
27 CSNK2B 1460
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
28 Tsc2 24855
Biochemical Activity Rattus norvegicus
29 SREK1 140890
Affinity Capture-MS Homo sapiens
30 TES 26136
Co-fractionation Homo sapiens
31 AGO2 27161
Biochemical Activity Homo sapiens
32 MAPK1 5594
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
33 CHMP4C 92421
Affinity Capture-MS Homo sapiens
34 RCSD1  
Biochemical Activity Homo sapiens
35 MAPK3 5595
PCA Homo sapiens
36 DDX21 9188
Affinity Capture-MS Homo sapiens
37 WASF1 8936
Reconstituted Complex Homo sapiens
38 PARK2  
Affinity Capture-MS Homo sapiens
39 ACIN1 22985
Affinity Capture-MS Homo sapiens
40 MAPKAPK3 7867
Affinity Capture-MS Homo sapiens
41 RSRC2  
Affinity Capture-MS Homo sapiens
42 CHMP4B 128866
Affinity Capture-MS Homo sapiens
43 DDX5 1655
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
44 PIAS2  
Biochemical Activity Homo sapiens
45 PLK1 5347
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
46 SCAF11  
Affinity Capture-MS Homo sapiens
47 ARPC5 10092
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
48 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
49 ETV1  
Biochemical Activity Homo sapiens
50 MAPK11  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 EGF 1950
Phenotypic Enhancement Homo sapiens
52 RBBP6 5930
Affinity Capture-MS Homo sapiens
53 PRPF4B 8899
Affinity Capture-MS Homo sapiens
54 XPO1 7514
Affinity Capture-MS Homo sapiens
55 WDR33 55339
Affinity Capture-MS Homo sapiens
56 CIR1  
Affinity Capture-MS Homo sapiens
57 CUL3 8452
Affinity Capture-MS Homo sapiens
58 YWHAZ 7534
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
59 YLPM1 56252
Affinity Capture-MS Homo sapiens
60 SLTM 79811
Affinity Capture-MS Homo sapiens
61 NXF1 10482
Affinity Capture-RNA Homo sapiens
62 HIBADH 11112
Co-fractionation Homo sapiens
63 MAPKAPK5 8550
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
64 CEP131 22994
Biochemical Activity Homo sapiens
65 PPP1R10  
Affinity Capture-MS Homo sapiens
66 DDRGK1 65992
Affinity Capture-MS Homo sapiens
67 ACY1 95
Co-fractionation Homo sapiens
68 PGAM1 5223
Co-fractionation Homo sapiens
69 AKT1 207
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
70 BRD9 65980
Affinity Capture-MS Homo sapiens
71 MDM2  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
72 UBE2J1 51465
Biochemical Activity Homo sapiens
73 DDX46 9879
Affinity Capture-MS Homo sapiens
74 Pde4a  
Biochemical Activity Rattus norvegicus
75 PAXBP1  
Affinity Capture-MS Homo sapiens
76 MTHFD2 10797
Affinity Capture-MS Homo sapiens
77 MAPKAPK2 9261
PCA Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
PCA Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
78 PDLIM7 9260
Co-fractionation Homo sapiens
79 MAP2K6 5608
Biochemical Activity Homo sapiens
80 PRRC2A 7916
Affinity Capture-MS Homo sapiens
81 DIDO1  
Affinity Capture-MS Homo sapiens
82 RFC1 5981
Affinity Capture-MS Homo sapiens
83 ZFP36  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
84 GBP1 2633
Co-fractionation Homo sapiens
85 WBP11  
Affinity Capture-MS Homo sapiens
86 RBM43 375287
Affinity Capture-MS Homo sapiens
87 PRRC2C 23215
Affinity Capture-MS Homo sapiens
88 C1orf35  
Affinity Capture-MS Homo sapiens
89 HSPB1 3315
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
90 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
91 SIRT1  
Affinity Capture-MS Homo sapiens
92 ATF1  
Biochemical Activity Homo sapiens
93 SUGP2  
Affinity Capture-MS Homo sapiens
94 ESR1  
Affinity Capture-MS Homo sapiens
95 SFSWAP  
Affinity Capture-MS Homo sapiens
96 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
97 MOV10 4343
Affinity Capture-RNA Homo sapiens
98 BECN1 8678
Biochemical Activity Homo sapiens
99 CIT 11113
Affinity Capture-MS Homo sapiens
100 PHF3  
Affinity Capture-MS Homo sapiens
101 DES 1674
Two-hybrid Homo sapiens
102 ZFP36L1  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
103 PHC2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
104 TRIM29 23650
Biochemical Activity Homo sapiens
105 MAGI1  
Affinity Capture-MS Homo sapiens
106 PRKRIR  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MAPKAPK2 is involved
PathwayEvidenceSource
activated TAK1 mediates p38 MAPK activation TAS Reactome
Arachidonate metabolism TAS Reactome
Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA TAS Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
CREB phosphorylation TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Death Receptor Signaling TAS Reactome
Fatty acid metabolism TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Interleukin-17 signaling TAS Reactome
MAP kinase activation TAS Reactome
MAPK targets/ Nuclear events mediated by MAP kinases TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Metabolism of RNA TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Nuclear Events (kinase and transcription factor activation) TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
p38MAPK events TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements TAS Reactome
Regulation of TNFR1 signaling TAS Reactome
Signal Transduction TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by NTRK1 (TRKA) TAS Reactome
Signaling by NTRKs TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by VEGF TAS Reactome
Signalling to ERKs TAS Reactome
Signalling to RAS TAS Reactome
Synthesis of Leukotrienes (LT) and Eoxins (EX) TAS Reactome
TNF signaling TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome
Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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