Gene description for CDK12
Gene name cyclin-dependent kinase 12
Gene symbol CDK12
Other names/aliases CRK7
CRKR
CRKRS
Species Homo sapiens
 Database cross references - CDK12
ExoCarta ExoCarta_51755
Vesiclepedia VP_51755
Entrez Gene 51755
HGNC 24224
MIM 615514
UniProt Q9NYV4  
 CDK12 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for CDK12
Molecular Function
    protein kinase activity GO:0004672 IDA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IBA
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IDA
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IMP
    protein kinase binding GO:0019901 IEA
    cyclin binding GO:0030332 IBA
    cyclin binding GO:0030332 IPI
    protein serine kinase activity GO:0106310 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 kinase activity GO:0140833 IEA
    RNA polymerase II CTD heptapeptide repeat S2 kinase activity GO:0140834 IEA
    RNA polymerase II CTD heptapeptide repeat T4 kinase activity GO:0140835 IEA
    RNA polymerase II CTD heptapeptide repeat S5 kinase activity GO:0140836 IEA
    RNA polymerase II CTD heptapeptide repeat S7 kinase activity GO:0140837 IEA
Biological Process
    transcription by RNA polymerase II GO:0006366 IDA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 ISS
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IBA
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IDA
    regulation of MAP kinase activity GO:0043405 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    protein autophosphorylation GO:0046777 IDA
    regulation of cell cycle GO:0051726 IEA
    negative regulation of stem cell differentiation GO:2000737 IEA
Subcellular Localization
    cyclin K-CDK12 complex GO:0002944 IPI
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cyclin/CDK positive transcription elongation factor complex GO:0008024 IBA
    nuclear speck GO:0016607 IDA
    nuclear cyclin-dependent protein kinase holoenzyme complex GO:0019908 ISS
 Experiment description of studies that identified CDK12 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDK12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 H2AFJ 55766
Affinity Capture-MS Homo sapiens
4 PAXIP1  
Affinity Capture-MS Homo sapiens
5 ZNF346  
Affinity Capture-MS Homo sapiens
6 SRPK2 6733
Affinity Capture-MS Homo sapiens
7 ACAA1 30
Co-fractionation Homo sapiens
8 Gtf2b  
Affinity Capture-MS Mus musculus
9 RPL13A 23521
Affinity Capture-MS Homo sapiens
10 RPL37A 6168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ACTC1 70
Proximity Label-MS Homo sapiens
12 DHX8 1659
Proximity Label-MS Homo sapiens
13 SPTLC1 10558
Affinity Capture-MS Homo sapiens
14 RPL15 6138
Affinity Capture-MS Homo sapiens
15 RPL14 9045
Affinity Capture-MS Homo sapiens
16 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
17 SCAF4 57466
Co-fractionation Homo sapiens
18 PRPF40A 55660
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
19 RPS3 6188
Affinity Capture-MS Homo sapiens
20 ANKRD50 57182
Affinity Capture-MS Homo sapiens
21 TCEB3 6924
Co-fractionation Homo sapiens
22 CCNK  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
23 DDX23 9416
Proximity Label-MS Homo sapiens
24 RNPS1 10921
Affinity Capture-MS Homo sapiens
25 PCGF1 84759
Affinity Capture-MS Homo sapiens
26 LASP1 3927
Affinity Capture-MS Homo sapiens
27 SRRM2 23524
Affinity Capture-MS Homo sapiens
28 YWHAG 7532
Affinity Capture-MS Homo sapiens
29 ZNF2  
Affinity Capture-MS Homo sapiens
30 EZH1  
Affinity Capture-MS Homo sapiens
31 ADARB1 104
Affinity Capture-MS Homo sapiens
32 PRKRA 8575
Affinity Capture-MS Homo sapiens
33 ATG16L1 55054
Affinity Capture-MS Homo sapiens
34 MECP2 4204
Affinity Capture-MS Homo sapiens
35 MYCN  
Affinity Capture-MS Homo sapiens
36 SERBP1 26135
Affinity Capture-MS Homo sapiens
37 EEF1A1 1915
Cross-Linking-MS (XL-MS) Homo sapiens
38 EWSR1 2130
Two-hybrid Homo sapiens
39 LIN28A  
Affinity Capture-MS Homo sapiens
40 TRIM24  
Co-fractionation Homo sapiens
41 IFI27L1  
Affinity Capture-MS Homo sapiens
42 TMEM14B  
Affinity Capture-MS Homo sapiens
43 ZNF606  
Co-fractionation Homo sapiens
44 RPL4 6124
Affinity Capture-MS Homo sapiens
45 VPS51 738
Co-fractionation Homo sapiens
46 HMGN2 3151
Co-fractionation Homo sapiens
47 SRSF6 6431
Affinity Capture-MS Homo sapiens
48 PURG  
Affinity Capture-MS Homo sapiens
49 NOP2 4839
Affinity Capture-MS Homo sapiens
50 CBX3 11335
Proximity Label-MS Homo sapiens
51 FGFBP1 9982
Affinity Capture-MS Homo sapiens
52 BARD1 580
Affinity Capture-MS Homo sapiens
53 FGF8  
Affinity Capture-MS Homo sapiens
54 RAD18  
Affinity Capture-MS Homo sapiens
55 RPL36 25873
Affinity Capture-MS Homo sapiens
56 IPPK  
Co-fractionation Homo sapiens
57 ANAPC15  
Affinity Capture-MS Homo sapiens
58 HECTD1 25831
Affinity Capture-MS Homo sapiens
59 RPLP0 6175
Affinity Capture-MS Homo sapiens
60 MAGEB2 4113
Affinity Capture-MS Homo sapiens
61 CDX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 MGMT 4255
Affinity Capture-MS Homo sapiens
63 BCLAF1 9774
Affinity Capture-MS Homo sapiens
64 TPX2  
Affinity Capture-MS Homo sapiens
65 RBM39 9584
Affinity Capture-MS Homo sapiens
66 ABT1 29777
Affinity Capture-MS Homo sapiens
67 BAZ1B 9031
Affinity Capture-MS Homo sapiens
68 RC3H2  
Affinity Capture-MS Homo sapiens
69 SRSF4 6429
Affinity Capture-MS Homo sapiens
70 SRSF1 6426
Affinity Capture-MS Homo sapiens
71 RPL21 6144
Cross-Linking-MS (XL-MS) Homo sapiens
72 MAP4K4 9448
Co-fractionation Homo sapiens
73 ERLEC1 27248
Affinity Capture-MS Homo sapiens
74 RPS8 6202
Affinity Capture-MS Homo sapiens
75 SRSF5 6430
Affinity Capture-MS Homo sapiens
76 RPL7 6129
Cross-Linking-MS (XL-MS) Homo sapiens
77 WEE1 7465
Synthetic Lethality Homo sapiens
78 AHSP  
Affinity Capture-MS Homo sapiens
79 MYC  
Dosage Lethality Homo sapiens
Affinity Capture-MS Homo sapiens
80 RPA3 6119
Proximity Label-MS Homo sapiens
81 RPS14 6208
Affinity Capture-MS Homo sapiens
82 CDK20  
Affinity Capture-MS Homo sapiens
83 DDB1 1642
FRET Homo sapiens
Reconstituted Complex Homo sapiens
84 DDRGK1 65992
Affinity Capture-MS Homo sapiens
85 SUPT16H 11198
Co-fractionation Homo sapiens
86 MALL  
Affinity Capture-MS Homo sapiens
87 RPL13 6137
Affinity Capture-MS Homo sapiens
88 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
89 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
90 WDR77 79084
Affinity Capture-MS Homo sapiens
91 RRS1 23212
Affinity Capture-MS Homo sapiens
92 PIN1 5300
Reconstituted Complex Homo sapiens
93 SAA1 6288
Affinity Capture-MS Homo sapiens
94 DGCR8  
Affinity Capture-MS Homo sapiens
95 IFI6  
Affinity Capture-MS Homo sapiens
96 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
97 NUP35 129401
Proximity Label-MS Homo sapiens
98 DDX58 23586
Affinity Capture-RNA Homo sapiens
99 C20orf197  
Affinity Capture-MS Homo sapiens
100 NUP50 10762
Proximity Label-MS Homo sapiens
101 CDK13 8621
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
102 CDK9 1025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 ZCCHC10  
Affinity Capture-MS Homo sapiens
104 RPS6 6194
Affinity Capture-MS Homo sapiens
105 RPL28 6158
Affinity Capture-MS Homo sapiens
106 XRCC6 2547
Proximity Label-MS Homo sapiens
107 PRPF4 9128
Co-fractionation Homo sapiens
108 SNW1 22938
Affinity Capture-MS Homo sapiens
109 KRAS 3845
Negative Genetic Homo sapiens
110 CYCS 54205
Affinity Capture-MS Homo sapiens
111 RC3H1 149041
Affinity Capture-MS Homo sapiens
112 UQCR11  
Affinity Capture-MS Homo sapiens
113 EP300 2033
Affinity Capture-MS Homo sapiens
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