Gene description for MGMT
Gene name O-6-methylguanine-DNA methyltransferase
Gene symbol MGMT
Other names/aliases -
Species Homo sapiens
 Database cross references - MGMT
ExoCarta ExoCarta_4255
Vesiclepedia VP_4255
Entrez Gene 4255
HGNC 7059
MIM 156569
UniProt P16455  
 MGMT identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MGMT
Molecular Function
    DNA binding GO:0003677 IEA
    methylated-DNA-[protein]-cysteine S-methyltransferase activity GO:0003908 IBA
    methyltransferase activity GO:0008168 TAS
    DNA-methyltransferase activity GO:0009008 TAS
    metal ion binding GO:0046872 IEA
Biological Process
    DNA ligation GO:0006266 TAS
    DNA repair GO:0006281 IBA
    DNA alkylation repair GO:0006307 IEA
    methylation GO:0032259 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    positive regulation of double-strand break repair GO:2000781 IDA
Subcellular Localization
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified MGMT in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MGMT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 H2BFM  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 FBL 2091
Affinity Capture-MS Homo sapiens
4 RELA 5970
Co-localization Homo sapiens
5 MCM3 4172
Affinity Capture-MS Homo sapiens
6 PHB 5245
Affinity Capture-MS Homo sapiens
7 HNRNPC 3183
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 SRP9 6726
Affinity Capture-MS Homo sapiens
10 ORC1  
Affinity Capture-Western Homo sapiens
11 FBXO27  
Affinity Capture-MS Homo sapiens
12 SLC25A6 293
Affinity Capture-MS Homo sapiens
13 EEF1A1 1915
Affinity Capture-MS Homo sapiens
14 MSH2 4436
Affinity Capture-MS Homo sapiens
15 YWHAZ 7534
Affinity Capture-MS Homo sapiens
16 PKM 5315
Affinity Capture-MS Homo sapiens
17 PSME2 5721
Affinity Capture-MS Homo sapiens
18 IL25  
Affinity Capture-MS Homo sapiens
19 SSRP1 6749
Affinity Capture-MS Homo sapiens
20 SP1  
Co-localization Homo sapiens
21 CDK1 983
Affinity Capture-Western Homo sapiens
22 CTNNB1 1499
Co-localization Homo sapiens
23 KPNB1 3837
Affinity Capture-MS Homo sapiens
24 ANXA1 301
Affinity Capture-MS Homo sapiens
25 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
26 PRKCH 5583
Affinity Capture-MS Homo sapiens
27 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
28 SET 6418
Affinity Capture-MS Homo sapiens
29 TUBB4B 10383
Affinity Capture-MS Homo sapiens
30 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
31 CSNK2A1 1457
Biochemical Activity Homo sapiens
32 FBXO2 26232
Affinity Capture-MS Homo sapiens
33 CDKL5  
Affinity Capture-MS Homo sapiens
34 CREBBP  
Affinity Capture-Western Homo sapiens
35 CDK2 1017
Affinity Capture-Western Homo sapiens
36 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
37 EEF1B2 1933
Affinity Capture-MS Homo sapiens
38 ANP32E 81611
Affinity Capture-MS Homo sapiens
39 MYO7A 4647
Affinity Capture-MS Homo sapiens
40 PRKDC 5591
Affinity Capture-MS Homo sapiens
41 HIST3H3 8290
Affinity Capture-MS Homo sapiens
42 ESR1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
43 CDKN1A  
Affinity Capture-Western Homo sapiens
44 ACTB 60
Affinity Capture-MS Homo sapiens
45 UBB 7314
Affinity Capture-MS Homo sapiens
46 PRKACA 5566
Biochemical Activity Homo sapiens
47 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
48 PTPN4  
Affinity Capture-MS Homo sapiens
49 BRCA2 675
Affinity Capture-Western Homo sapiens
50 CCNB1 891
Affinity Capture-Western Homo sapiens
51 NCL 4691
Affinity Capture-MS Homo sapiens
52 TUBA1C 84790
Affinity Capture-MS Homo sapiens
53 SLC25A4 291
Affinity Capture-MS Homo sapiens
54 DDX5 1655
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
55 DDX24 57062
Affinity Capture-MS Homo sapiens
56 PRKCA 5578
Biochemical Activity Homo sapiens
57 CSNK2A2 1459
Biochemical Activity Homo sapiens
58 TOP1 7150
Affinity Capture-MS Homo sapiens
59 USP19 10869
Affinity Capture-MS Homo sapiens
60 EEF1A2 1917
Affinity Capture-MS Homo sapiens
61 RPLP1 6176
Affinity Capture-MS Homo sapiens
62 CDK12 51755
Affinity Capture-MS Homo sapiens
63 XRCC6 2547
Affinity Capture-MS Homo sapiens
64 PRPF4 9128
Affinity Capture-MS Homo sapiens
65 PSMD9 5715
Affinity Capture-MS Homo sapiens
66 SMC1A 8243
Affinity Capture-MS Homo sapiens
67 POLD1 5424
Affinity Capture-Western Homo sapiens
68 WNT9B  
Affinity Capture-MS Homo sapiens
69 PCNA 5111
Affinity Capture-Western Homo sapiens
70 ANXA2 302
Affinity Capture-MS Homo sapiens
71 RNF8  
Affinity Capture-MS Homo sapiens
72 VIM 7431
Affinity Capture-MS Homo sapiens
73 CDK10  
Affinity Capture-MS Homo sapiens
74 PABPC3 5042
Affinity Capture-MS Homo sapiens
75 RPS15A 6210
Affinity Capture-MS Homo sapiens
76 FANCA  
Affinity Capture-Luminescence Homo sapiens
77 MCM2 4171
Affinity Capture-Western Homo sapiens
78 EP300 2033
Affinity Capture-Western Homo sapiens
79 TTPA  
Affinity Capture-MS Homo sapiens
80 EIF3H 8667
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MGMT is involved
PathwayEvidenceSource
DNA Damage Reversal TAS Reactome
DNA Repair TAS Reactome
MGMT-mediated DNA damage reversal TAS Reactome





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