Gene description for MYO7A
Gene name myosin VIIA
Gene symbol MYO7A
Other names/aliases DFNA11
DFNB2
MYOVIIA
MYU7A
NSRD2
USH1B
Species Homo sapiens
 Database cross references - MYO7A
ExoCarta ExoCarta_4647
Vesiclepedia VP_4647
Entrez Gene 4647
HGNC 7606
MIM 276903
UniProt Q13402  
 MYO7A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MYO7A
Molecular Function
    microfilament motor activity GO:0000146 IBA
    microfilament motor activity GO:0000146 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IMP
    ATP binding GO:0005524 IEA
    protein domain specific binding GO:0019904 IEA
    spectrin binding GO:0030507 IDA
    identical protein binding GO:0042802 IEA
    ADP binding GO:0043531 IEA
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
Biological Process
    phagolysosome assembly GO:0001845 IEA
    intracellular protein transport GO:0006886 IEA
    endocytosis GO:0006897 IBA
    actin filament organization GO:0007015 IBA
    lysosome organization GO:0007040 IDA
    sensory organ development GO:0007423 IBA
    visual perception GO:0007601 IMP
    sensory perception of sound GO:0007605 IBA
    sensory perception of sound GO:0007605 IMP
    sensory perception of sound GO:0007605 IMP
    protein localization GO:0008104 ISS
    actin filament-based movement GO:0030048 IBA
    actin filament-based movement GO:0030048 IDA
    eye photoreceptor cell development GO:0042462 IC
    mechanoreceptor differentiation GO:0042490 ISS
    sensory perception of light stimulus GO:0050953 IMP
    equilibrioception GO:0050957 IMP
    pigment granule transport GO:0051904 IEA
    auditory receptor cell stereocilium organization GO:0060088 IEA
    cochlea development GO:0090102 IEA
Subcellular Localization
    photoreceptor outer segment GO:0001750 IDA
    photoreceptor inner segment GO:0001917 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    lysosomal membrane GO:0005765 IDA
    cytosol GO:0005829 IDA
    microvillus GO:0005902 IBA
    cell cortex GO:0005938 IEA
    actin cytoskeleton GO:0015629 IBA
    membrane GO:0016020 IBA
    apical plasma membrane GO:0016324 IEA
    myosin VII complex GO:0031477 IEA
    photoreceptor connecting cilium GO:0032391 IEA
    stereocilium GO:0032420 ISS
    melanosome GO:0042470 IEA
    synapse GO:0045202 IDA
    stereocilium base GO:0120044 ISS
    upper tip-link density GO:1990435 IEA
 Experiment description of studies that identified MYO7A in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 237
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MYO7A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDH1 999
Affinity Capture-Western Homo sapiens
2 USH1C  
Reconstituted Complex Homo sapiens
3 CTNNB1 1499
Affinity Capture-Western Homo sapiens
4 KEAP1 9817
Two-hybrid Homo sapiens
5 PRKAR1A 5573
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 KHDRBS1 10657
Protein-peptide Homo sapiens
7 FAM65A 79567
Affinity Capture-MS Homo sapiens
8 CTNNA1 1495
Affinity Capture-Western Homo sapiens
9 LANCL1 10314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MKRN2 23609
Affinity Capture-RNA Homo sapiens
11 SLK 9748
Cross-Linking-MS (XL-MS) Homo sapiens
12 MAP2 4133
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 CALM1 801
Reconstituted Complex Homo sapiens
14 CANX 821
Cross-Linking-MS (XL-MS) Homo sapiens
15 VEZT 55591
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
16 CUL9  
Affinity Capture-MS Homo sapiens
17 NR3C1 2908
Proximity Label-MS Homo sapiens
18 RDX 5962
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
19 LINC00839  
Protein-RNA Homo sapiens
20 MGMT 4255
Affinity Capture-MS Homo sapiens
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