Gene description for MAPKAPK3
Gene name mitogen-activated protein kinase-activated protein kinase 3
Gene symbol MAPKAPK3
Other names/aliases 3PK
MAPKAP-K3
MAPKAP3
MAPKAPK-3
MK-3
Species Homo sapiens
 Database cross references - MAPKAPK3
ExoCarta ExoCarta_7867
Vesiclepedia VP_7867
Entrez Gene 7867
HGNC 6888
MIM 602130
UniProt Q16644  
 MAPKAPK3 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAPKAPK3
Molecular Function
    protein serine/threonine kinase activity GO:0004674 EXP
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    calcium/calmodulin-dependent protein kinase activity GO:0004683 IBA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    MAP kinase kinase activity GO:0004708 TAS
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    ATP binding GO:0005524 IEA
    calcium-dependent protein serine/threonine kinase activity GO:0009931 IBA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    mitogen-activated protein kinase binding GO:0051019 IBA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    MAPK cascade GO:0000165 IEA
    toll-like receptor signaling pathway GO:0002224 IBA
    toll-like receptor signaling pathway GO:0002224 ISS
    chromatin remodeling GO:0006338 IEA
    signal transduction GO:0007165 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    response to lipopolysaccharide GO:0032496 ISS
    response to cytokine GO:0034097 IBA
    response to cytokine GO:0034097 IDA
    intracellular signal transduction GO:0035556 IBA
    macropinocytosis GO:0044351 ISS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MAPKAPK3 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAPKAPK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BECN1 8678
Biochemical Activity Homo sapiens
2 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
3 PLS3 5358
Co-fractionation Homo sapiens
4 NEDD4 4734
Biochemical Activity Homo sapiens
5 RCSD1  
Biochemical Activity Homo sapiens
6 MAPK13 5603
Co-localization Homo sapiens
7 IGHM 3507
Two-hybrid Homo sapiens
8 TCF3  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
9 HSPB1 3315
Co-localization Homo sapiens
10 DIEXF  
Affinity Capture-MS Homo sapiens
11 ATF1  
Biochemical Activity Homo sapiens
12 APP 351
Reconstituted Complex Homo sapiens
13 PTPN7 5778
Affinity Capture-MS Homo sapiens
14 PTPN5  
Affinity Capture-MS Homo sapiens
15 RAB2A 5862
Two-hybrid Homo sapiens
16 EZH2  
Two-hybrid Homo sapiens
17 Nedd4 25489
Biochemical Activity Rattus norvegicus
18 PAPSS1 9061
Co-fractionation Homo sapiens
19 COPS6 10980
Two-hybrid Homo sapiens
20 PTPDC1  
Affinity Capture-MS Homo sapiens
21 NEDD4L 23327
Biochemical Activity Homo sapiens
22 MEF2A  
Affinity Capture-MS Homo sapiens
23 NUDT3 11165
Co-fractionation Homo sapiens
24 PRKY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RNF2  
Affinity Capture-Western Homo sapiens
26 BMI1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 SMS 6611
Two-hybrid Homo sapiens
28 PHC2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
29 MAPK14 1432
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
30 WARS 7453
Co-fractionation Homo sapiens
31 PTPRR  
Affinity Capture-MS Homo sapiens
32 SUMO3 6612
Two-hybrid Homo sapiens
33 PRKRIR  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
34 MAPK11  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MAPKAPK3 is involved
PathwayEvidenceSource
activated TAK1 mediates p38 MAPK activation TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Interleukin-17 signaling TAS Reactome
MAP kinase activation TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
p38MAPK events TAS Reactome
Signal Transduction TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by NTRK1 (TRKA) TAS Reactome
Signaling by NTRKs TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by VEGF TAS Reactome
Signalling to ERKs TAS Reactome
Signalling to RAS TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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