Gene description for EGF
Gene name epidermal growth factor
Gene symbol EGF
Other names/aliases HOMG4
URG
Species Homo sapiens
 Database cross references - EGF
ExoCarta ExoCarta_1950
Vesiclepedia VP_1950
Entrez Gene 1950
HGNC 3229
MIM 131530
UniProt P01133  
 EGF identified in sEVs derived from the following tissue/cell type
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Plasma 36631249    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 15326289    
Urine 19056867    
Urine 21082674    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for EGF
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    epidermal growth factor receptor binding GO:0005154 TAS
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IBA
    growth factor activity GO:0008083 IDA
    transmembrane receptor protein tyrosine kinase activator activity GO:0030297 IDA
    transmembrane receptor protein tyrosine kinase activator activity GO:0030297 TAS
    receptor ligand activity GO:0048018 IDA
Biological Process
    angiogenesis GO:0001525 IDA
    positive regulation of endothelial cell proliferation GO:0001938 IDA
    positive regulation of receptor internalization GO:0002092 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 IMP
    positive regulation of cell population proliferation GO:0008284 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IMP
    positive regulation of endothelial cell migration GO:0010595 IDA
    positive regulation of gene expression GO:0010628 IDA
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IDA
    cerebellar granule cell precursor proliferation GO:0021930 IEA
    positive regulation of cerebellar granule cell precursor proliferation GO:0021940 IEA
    positive regulation of cell migration GO:0030335 IDA
    ERBB2-EGFR signaling pathway GO:0038134 IDA
    positive regulation of phosphorylation GO:0042327 IDA
    positive regulation of DNA binding GO:0043388 ISS
    positive regulation of MAPK cascade GO:0043410 IBA
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of MAPK cascade GO:0043410 IMP
    positive regulation of mitotic nuclear division GO:0045840 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    regulation of receptor signaling pathway via JAK-STAT GO:0046425 ISS
    branching morphogenesis of an epithelial tube GO:0048754 IEA
    epithelial cell proliferation GO:0050673 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    negative regulation of secretion GO:0051048 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IGI
    mammary gland alveolus development GO:0060749 IEA
    ERK1 and ERK2 cascade GO:0070371 IDA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IDA
    regulation of calcium ion import GO:0090279 IDA
    negative regulation of cholesterol efflux GO:0090370 IEA
    positive regulation of hyaluronan biosynthetic process GO:1900127 IDA
    positive regulation of protein localization to early endosome GO:1902966 IDA
    positive regulation of epithelial tube formation GO:1905278 IDA
    regulation of protein localization to cell surface GO:2000008 IDA
    positive regulation of ubiquitin-dependent protein catabolic process GO:2000060 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    lysosomal membrane GO:0005765 HDA
    plasma membrane GO:0005886 IBA
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    platelet alpha granule lumen GO:0031093 TAS
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified EGF in sEVs
1
Experiment ID 1371
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 1372
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 1373
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 1374
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 1377
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 1378
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 1381
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 1382
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 1383
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 1385
MISEV standards
Biophysical techniques
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 1387
MISEV standards
Biophysical techniques
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 1388
MISEV standards
Biophysical techniques
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 1370
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Immuno-EM
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Plasma
Sample name Hepatocellular carcinoma patients
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
14
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
18
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
19
Experiment ID 102
MISEV standards
✔
IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21082674    
Organism Homo sapiens
Experiment description "Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS."
Authors "Zhang Y, Li Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
20
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EGF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VTN 7448
Reconstituted Complex Homo sapiens
2 APP 351
Affinity Capture-Western Homo sapiens
3 EGF 1950
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
4 UBE2I 7329
Affinity Capture-Western Homo sapiens
5 PCGF2 7703
Affinity Capture-Western Homo sapiens
6 RPS6KA5 9252
Phenotypic Enhancement Homo sapiens
Co-localization Homo sapiens
7 APEX1 328
Affinity Capture-RNA Homo sapiens
8 MAPKAPK2 9261
Phenotypic Enhancement Homo sapiens
9 FNBP1 23048
Co-localization Homo sapiens
10 ZFYVE28  
Co-localization Homo sapiens
11 FGF5  
Co-localization Homo sapiens
12 ERBB2 2064
Reconstituted Complex Homo sapiens
13 SREBF1 6720
Affinity Capture-Western Homo sapiens
14 EMD 2010
Affinity Capture-Western Homo sapiens
15 EGFR 1956
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
16 SHC1 6464
Affinity Capture-Western Homo sapiens
17 ERBB3 2065
Reconstituted Complex Homo sapiens
18 LMNB1 4001
Affinity Capture-Western Homo sapiens
19 LMNA 4000
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which EGF is involved
PathwayEvidenceSource
Cargo recognition for clathrin-mediated endocytosis TAS Reactome
Cellular response to chemical stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Clathrin-mediated endocytosis TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Constitutive Signaling by EGFRvIII TAS Reactome
Constitutive Signaling by Ligand-Responsive EGFR Cancer Variants TAS Reactome
Developmental Biology TAS Reactome
Developmental Cell Lineages TAS Reactome
Differentiation of keratinocytes in interfollicular epidermis in mammalian skin TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downregulation of ERBB2 signaling TAS Reactome
EGFR downregulation TAS Reactome
EGFR interacts with phospholipase C-gamma TAS Reactome
ERBB2 Activates PTK6 Signaling TAS Reactome
ERBB2 Regulates Cell Motility TAS Reactome
ESR-mediated signaling TAS Reactome
Estrogen-dependent nuclear events downstream of ESR-membrane signaling TAS Reactome
Extra-nuclear estrogen signaling TAS Reactome
GAB1 signalosome TAS Reactome
GRB2 events in EGFR signaling TAS Reactome
GRB2 events in ERBB2 signaling IEA Reactome
Hemostasis TAS Reactome
Inhibition of Signaling by Overexpressed EGFR TAS Reactome
Intracellular signaling by second messengers TAS Reactome
KEAP1-NFE2L2 pathway TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Membrane Trafficking TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
NFE2L2 regulating tumorigenic genes TAS Reactome
NOTCH3 Activation and Transmission of Signal to the Nucleus TAS Reactome
Nuclear events mediated by NFE2L2 TAS Reactome
PI3K events in ERBB2 signaling TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet degranulation TAS Reactome
PLCG1 events in ERBB2 signaling IEA Reactome
RAF/MAP kinase cascade TAS Reactome
Response to elevated platelet cytosolic Ca2+ TAS Reactome
SHC1 events in EGFR signaling TAS Reactome
SHC1 events in ERBB2 signaling IEA Reactome
SHC1 events in ERBB2 signaling TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by EGFR IEA Reactome
Signaling by EGFR TAS Reactome
Signaling by EGFR in Cancer TAS Reactome
Signaling by EGFRvIII in Cancer TAS Reactome
Signaling by ERBB2 TAS Reactome
Signaling by ERBB2 IEA Reactome
Signaling by ERBB2 ECD mutants TAS Reactome
Signaling by ERBB2 in Cancer TAS Reactome
Signaling by ERBB2 KD Mutants TAS Reactome
Signaling by ERBB2 TMD/JMD mutants TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by Ligand-Responsive EGFR Variants in Cancer TAS Reactome
Signaling by Non-Receptor Tyrosine Kinases TAS Reactome
Signaling by NOTCH TAS Reactome
Signaling by NOTCH3 TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by Overexpressed Wild-Type EGFR in Cancer TAS Reactome
Signaling by PTK6 TAS Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Vesicle-mediated transport TAS Reactome





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