Gene description for ACIN1
Gene name apoptotic chromatin condensation inducer 1
Gene symbol ACIN1
Other names/aliases ACINUS
ACN
fSAP152
Species Homo sapiens
 Database cross references - ACIN1
ExoCarta ExoCarta_22985
Vesiclepedia VP_22985
Entrez Gene 22985
HGNC 17066
MIM 604562
UniProt Q9UKV3  
 ACIN1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Hepatocytes 26054723    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
 Gene ontology annotations for ACIN1
Molecular Function
    nucleic acid binding GO:0003676 NAS
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    ATP hydrolysis activity GO:0016887 NAS
    enzyme binding GO:0019899 NAS
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IBA
    erythrocyte differentiation GO:0030218 IEP
    apoptotic chromosome condensation GO:0030263 IDA
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of monocyte differentiation GO:0045657 IEP
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    nuclear speck GO:0016607 IDA
    exon-exon junction complex GO:0035145 IDA
    ASAP complex GO:0061574 IBA
    ASAP complex GO:0061574 IDA
 Experiment description of studies that identified ACIN1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
16
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for ACIN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFAP20 29105
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LDHA 3939
Cross-Linking-MS (XL-MS) Homo sapiens
5 ABCF3 55324
Two-hybrid Homo sapiens
6 UBE2H 7328
Affinity Capture-MS Homo sapiens
7 LUZP4  
Affinity Capture-MS Homo sapiens
8 SENP3 26168
Affinity Capture-MS Homo sapiens
9 E2F4  
Affinity Capture-MS Homo sapiens
10 KPNA3 3839
Co-fractionation Homo sapiens
11 LGR4 55366
Affinity Capture-MS Homo sapiens
12 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 EEF1G 1937
Co-fractionation Homo sapiens
14 ANLN 54443
Affinity Capture-MS Homo sapiens
15 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
16 PPIE 10450
Affinity Capture-MS Homo sapiens
17 SRPK2 6733
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
18 RPA2 6118
Proximity Label-MS Homo sapiens
19 DDX17 10521
Co-fractionation Homo sapiens
20 ACTC1 70
Proximity Label-MS Homo sapiens
21 SF3B1 23451
Affinity Capture-MS Homo sapiens
22 BRD4 23476
Affinity Capture-MS Homo sapiens
23 BRD2  
Affinity Capture-MS Homo sapiens
24 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
25 CSNK2A1 1457
Biochemical Activity Homo sapiens
26 SCAF4 57466
Cross-Linking-MS (XL-MS) Homo sapiens
27 PRPF40A 55660
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SNRPF 6636
Affinity Capture-MS Homo sapiens
29 SEPT2 4735
Co-fractionation Homo sapiens
30 GSK3A 2931
Affinity Capture-MS Homo sapiens
31 GOLGA7 51125
Affinity Capture-MS Homo sapiens
32 SAFB 6294
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
33 CUL7 9820
Affinity Capture-MS Homo sapiens
34 DDX23 9416
Proximity Label-MS Homo sapiens
35 RNPS1 10921
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
36 GNS 2799
Co-fractionation Homo sapiens
37 SULF2 55959
Affinity Capture-MS Homo sapiens
38 DENND2D  
Affinity Capture-MS Homo sapiens
39 SSRP1 6749
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
40 KIF23 9493
Affinity Capture-MS Homo sapiens
41 NFKBIL1  
Affinity Capture-MS Homo sapiens
42 TRMT112 51504
Co-fractionation Homo sapiens
43 PCBD1 5092
Reconstituted Complex Homo sapiens
44 PRC1 9055
Affinity Capture-MS Homo sapiens
45 IQGAP1 8826
Affinity Capture-MS Homo sapiens
46 TMEM184B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SNRPC 6631
Affinity Capture-MS Homo sapiens
48 OBSL1 23363
Affinity Capture-MS Homo sapiens
49 SF3A2 8175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SRP14 6727
Co-fractionation Homo sapiens
51 NELFE 7936
Affinity Capture-MS Homo sapiens
52 RCC1 1104
Co-fractionation Homo sapiens
53 SARNP 84324
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 U2AF1 7307
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 BET1 10282
Co-fractionation Homo sapiens
56 MECP2 4204
Affinity Capture-MS Homo sapiens
57 MYCN  
Affinity Capture-MS Homo sapiens
58 RBM42  
Affinity Capture-MS Homo sapiens
59 RRP7A 27341
Co-fractionation Homo sapiens
60 RBM14 10432
Affinity Capture-MS Homo sapiens
61 RBM8A 9939
Affinity Capture-MS Homo sapiens
62 KIF14 9928
Affinity Capture-MS Homo sapiens
63 SUB1 10923
Co-fractionation Homo sapiens
64 CHMP4C 92421
Affinity Capture-MS Homo sapiens
65 AURKA 6790
Affinity Capture-MS Homo sapiens
66 CLK2 1196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 DLD 1738
Affinity Capture-MS Homo sapiens
68 UTP14A 10813
Co-fractionation Homo sapiens
69 COX15 1355
Affinity Capture-MS Homo sapiens
70 DDX21 9188
Affinity Capture-MS Homo sapiens
71 FUT8 2530
Co-fractionation Homo sapiens
72 PARK2  
Affinity Capture-MS Homo sapiens
73 SNAP91 9892
Co-fractionation Homo sapiens
74 SUPT5H 6829
Affinity Capture-MS Homo sapiens
75 SNRPA 6626
Affinity Capture-MS Homo sapiens
76 SMU1 55234
Co-fractionation Homo sapiens
77 ATP7A 538
Co-fractionation Homo sapiens
78 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
79 SRSF6 6431
Affinity Capture-MS Homo sapiens
80 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
81 SRRM2 23524
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
82 PTCD3 55037
Co-fractionation Homo sapiens
83 SNRPB 6628
Affinity Capture-MS Homo sapiens
84 SNIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 NFIA 4774
Co-fractionation Homo sapiens
86 GRAMD4 23151
Affinity Capture-MS Homo sapiens
87 YAP1 10413
Affinity Capture-MS Homo sapiens
88 C11orf58  
Co-fractionation Homo sapiens
89 NTRK1 4914
Affinity Capture-MS Homo sapiens
90 U2AF2 11338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 RAD18  
Affinity Capture-MS Homo sapiens
92 CPSF6 11052
Affinity Capture-MS Homo sapiens
93 LUC7L2 51631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 BANF1 8815
Affinity Capture-MS Homo sapiens
95 EVPL 2125
Co-fractionation Homo sapiens
96 SMC1A 8243
Co-fractionation Homo sapiens
97 DHX9 1660
Co-fractionation Homo sapiens
98 HECTD1 25831
Affinity Capture-MS Homo sapiens
99 MAGOH 4116
Affinity Capture-MS Homo sapiens
100 INSL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 SRPK3  
Affinity Capture-MS Homo sapiens
102 SON 6651
Co-fractionation Homo sapiens
103 DDX39B 7919
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 SDPR 8436
Co-fractionation Homo sapiens
105 DNM1L 10059
Affinity Capture-MS Homo sapiens
106 SRPK1 6732
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 SLC25A5 292
Cross-Linking-MS (XL-MS) Homo sapiens
108 SNRPB2 6629
Affinity Capture-MS Homo sapiens
109 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 RTCB 51493
Affinity Capture-MS Homo sapiens
111 SRSF11 9295
Affinity Capture-MS Homo sapiens
112 RBBP6 5930
Affinity Capture-MS Homo sapiens
113 Rab5c 19345
Affinity Capture-MS Mus musculus
114 EPB41L1 2036
Affinity Capture-MS Homo sapiens
115 PABPC5 140886
Affinity Capture-MS Homo sapiens
116 ZBTB2 57621
Affinity Capture-MS Homo sapiens
117 RBM39 9584
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 PRPF4B 8899
Affinity Capture-MS Homo sapiens
119 C11orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 PRKCA 5578
Co-fractionation Homo sapiens
121 CLK3  
Affinity Capture-MS Homo sapiens
122 ECT2 1894
Affinity Capture-MS Homo sapiens
123 SIRT7  
Affinity Capture-MS Homo sapiens
124 SRSF4 6429
Affinity Capture-MS Homo sapiens
125 Esrrb  
Affinity Capture-MS Mus musculus
126 Ccdc9  
Affinity Capture-MS Mus musculus
127 CUL3 8452
Affinity Capture-MS Homo sapiens
128 CD9 928
Co-fractionation Homo sapiens
129 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
130 FUS 2521
Affinity Capture-MS Homo sapiens
131 IKZF1  
Affinity Capture-MS Homo sapiens
132 RPL7 6129
Co-fractionation Homo sapiens
133 ZC3H11A  
Affinity Capture-MS Homo sapiens
134 SUZ12  
Affinity Capture-MS Homo sapiens
135 SF3A1 10291
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
136 SGTB  
Affinity Capture-MS Homo sapiens
137 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 CAV1 857
Co-fractionation Homo sapiens
139 PCBP1 5093
Affinity Capture-MS Homo sapiens
140 MYC  
Affinity Capture-MS Homo sapiens
141 RPA3 6119
Proximity Label-MS Homo sapiens
142 RPS13 6207
Co-fractionation Homo sapiens
143 ZC3H18  
Co-fractionation Homo sapiens
144 DHRS7B 25979
Co-fractionation Homo sapiens
145 DLST 1743
Affinity Capture-MS Homo sapiens
146 TOP1 7150
Affinity Capture-MS Homo sapiens
147 EED  
Affinity Capture-MS Homo sapiens
148 WSB2 55884
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 LGMN 5641
Co-fractionation Homo sapiens
150 RNF2  
Affinity Capture-MS Homo sapiens
151 BMI1  
Affinity Capture-MS Homo sapiens
152 IKZF3  
Affinity Capture-MS Homo sapiens
153 SEPT7 989
Co-fractionation Homo sapiens
154 ARGLU1  
Affinity Capture-MS Homo sapiens
155 BRD3 8019
Affinity Capture-MS Homo sapiens
156 SUPT16H 11198
Co-fractionation Homo sapiens
157 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 NKAP 79576
Affinity Capture-MS Homo sapiens
159 Lgals3bp 19039
Affinity Capture-MS Mus musculus
160 DHX15 1665
Co-fractionation Homo sapiens
161 GRK5 2869
Affinity Capture-MS Homo sapiens
162 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
163 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
164 RNF151  
Affinity Capture-MS Homo sapiens
165 BCAS2 10286
Co-fractionation Homo sapiens
166 ZRANB2 9406
Affinity Capture-MS Homo sapiens
167 SRRM1 10250
Affinity Capture-MS Homo sapiens
168 RRS1 23212
Co-fractionation Homo sapiens
169 WWOX 51741
Affinity Capture-MS Homo sapiens
170 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
171 PAFAH1B2 5049
Two-hybrid Homo sapiens
172 PHRF1  
Affinity Capture-MS Homo sapiens
173 SAP18 10284
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
174 BOP1 23246
Co-fractionation Homo sapiens
175 PDHA1 5160
Affinity Capture-MS Homo sapiens
176 ARMCX3 51566
Co-fractionation Homo sapiens
177 LDHB 3945
Cross-Linking-MS (XL-MS) Homo sapiens
178 THRAP3 9967
Co-fractionation Homo sapiens
179 C1orf35  
Affinity Capture-MS Homo sapiens
180 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
181 CHD4 1108
Affinity Capture-MS Homo sapiens
182 RHOT2 89941
Co-fractionation Homo sapiens
183 EIF4A3 9775
Affinity Capture-MS Homo sapiens
184 EPB41L2 2037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
185 S100A9 6280
Co-fractionation Homo sapiens
186 DDX58 23586
Affinity Capture-RNA Homo sapiens
187 MKI67  
Affinity Capture-MS Homo sapiens
188 CUL9  
Affinity Capture-MS Homo sapiens
189 UFL1 23376
Affinity Capture-MS Homo sapiens
190 SNRPGP15  
Co-fractionation Homo sapiens
191 EPB41L3 23136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
192 API5 8539
Affinity Capture-MS Homo sapiens
193 ALYREF 10189
Affinity Capture-MS Homo sapiens
194 TECTB  
Affinity Capture-MS Homo sapiens
195 WIBG 84305
Co-fractionation Homo sapiens
196 ARRB1 408
Affinity Capture-MS Homo sapiens
197 YBX3 8531
Co-fractionation Homo sapiens
198 SRSF7 6432
Affinity Capture-MS Homo sapiens
199 PINK1  
Affinity Capture-MS Homo sapiens
200 PRPF6 24148
Co-fractionation Homo sapiens
201 SNW1 22938
Affinity Capture-MS Homo sapiens
202 KRAS 3845
Affinity Capture-MS Homo sapiens
203 SNRPA1 6627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
204 MEN1 4221
Affinity Capture-MS Homo sapiens
205 RBM33  
Affinity Capture-MS Homo sapiens
206 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
207 CSGALNACT1 55790
Co-fractionation Homo sapiens
208 NDUFAF4 29078
Co-fractionation Homo sapiens
209 SLC3A2 6520
Co-fractionation Homo sapiens
View the network image/svg+xml



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