Gene description for ARMCX3
Gene name armadillo repeat containing, X-linked 3
Gene symbol ARMCX3
Other names/aliases ALEX3
GASP6
dJ545K15.2
Species Homo sapiens
 Database cross references - ARMCX3
ExoCarta ExoCarta_51566
Vesiclepedia VP_51566
Entrez Gene 51566
HGNC 24065
MIM 300364
UniProt Q9UH62  
 ARMCX3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ARMCX3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitochondrion organization GO:0007005 IEA
    protein localization GO:0008104 IEA
    axonal transport of mitochondrion GO:0019896 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial outer membrane GO:0005741 IEA
    cytosol GO:0005829 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified ARMCX3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ARMCX3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POR 5447
Co-fractionation Homo sapiens
2 MTCH1 23787
Proximity Label-MS Homo sapiens
3 NDUFS1 4719
Co-fractionation Homo sapiens
4 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RPL15 6138
Affinity Capture-MS Homo sapiens
6 CSNK2A1 1457
Biochemical Activity Homo sapiens
7 ADAMTS13 11093
Affinity Capture-MS Homo sapiens
8 VWA5A 4013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SEPT2 4735
Co-fractionation Homo sapiens
10 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 APP 351
Reconstituted Complex Homo sapiens
12 ANKRD50 57182
Affinity Capture-MS Homo sapiens
13 HCCS 3052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LRPPRC 10128
Co-fractionation Homo sapiens
15 FIS1 51024
Proximity Label-MS Homo sapiens
16 NDUFS3 4722
Co-fractionation Homo sapiens
17 PRDX3 10935
Co-fractionation Homo sapiens
18 HYOU1 10525
Co-fractionation Homo sapiens
19 CLEC2B  
Affinity Capture-MS Homo sapiens
20 NPM1 4869
Co-fractionation Homo sapiens
21 THTPA  
Affinity Capture-MS Homo sapiens
22 FBL 2091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 LONP1 9361
Co-fractionation Homo sapiens
24 ATP2A3 489
Affinity Capture-MS Homo sapiens
25 LACTB 114294
Co-fractionation Homo sapiens
26 SLC25A46 91137
Proximity Label-MS Homo sapiens
27 NDUFV1 4723
Co-fractionation Homo sapiens
28 CLTC 1213
Two-hybrid Homo sapiens
29 JCHAIN 3512
Affinity Capture-MS Homo sapiens
30 GPN1  
Affinity Capture-MS Homo sapiens
31 LRRC17 10234
Affinity Capture-MS Homo sapiens
32 FAM177A1 283635
Affinity Capture-MS Homo sapiens
33 NDUFS5 4725
Co-fractionation Homo sapiens
34 ACIN1 22985
Co-fractionation Homo sapiens
35 EMC1 23065
Co-fractionation Homo sapiens
36 LMAN1 3998
Co-fractionation Homo sapiens
37 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NNT 23530
Co-fractionation Homo sapiens
39 HAUS2  
Affinity Capture-MS Homo sapiens
40 BCAR1 9564
Co-fractionation Homo sapiens
41 MTCH2 23788
Proximity Label-MS Homo sapiens
42 P4HA1 5033
Co-fractionation Homo sapiens
43 TUBA1A 7846
Co-fractionation Homo sapiens
44 GNAT2 2780
Affinity Capture-MS Homo sapiens
45 VCP 7415
Co-fractionation Homo sapiens
46 ZMPSTE24 10269
Co-fractionation Homo sapiens
47 CYC1 1537
Co-fractionation Homo sapiens
48 UQCRC1 7384
Co-fractionation Homo sapiens
49 SMC1A 8243
Co-fractionation Homo sapiens
50 PML 5371
Affinity Capture-MS Homo sapiens
51 BRAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 UQCRH 7388
Co-fractionation Homo sapiens
53 RANBP6 26953
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RHOT2 89941
Proximity Label-MS Homo sapiens
55 ATP5B 506
Co-fractionation Homo sapiens
56 RMDN3 55177
Proximity Label-MS Homo sapiens
57 CCDC59  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 P2RX4 5025
Affinity Capture-MS Homo sapiens
59 EPDR1 54749
Affinity Capture-MS Homo sapiens
60 IPO5 3843
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CANX 821
Co-fractionation Homo sapiens
62 LRRC59 55379
Co-fractionation Homo sapiens
63 MAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 UQCRC2 7385
Co-fractionation Homo sapiens
65 HMOX2 3163
Co-fractionation Homo sapiens
66 C2CD4B  
Affinity Capture-MS Homo sapiens
67 EEF1B2 1933
Co-fractionation Homo sapiens
68 OCIAD1 54940
Proximity Label-MS Homo sapiens
69 AMY1C 278
Affinity Capture-MS Homo sapiens
70 KCNE3  
Affinity Capture-MS Homo sapiens
71 FAM25G  
Two-hybrid Homo sapiens
72 GNAS 2778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ATP2A1 487
Affinity Capture-MS Homo sapiens
74 ATP6V1A 523
Co-fractionation Homo sapiens
75 RCN2 5955
Co-fractionation Homo sapiens
76 RANBP1 5902
Affinity Capture-MS Homo sapiens
77 KTN1 3895
Co-fractionation Homo sapiens
78 GANAB 23193
Co-fractionation Homo sapiens
79 NDUFV2 4729
Co-fractionation Homo sapiens
80 AKAP1 8165
Proximity Label-MS Homo sapiens
81 IMMT 10989
Two-hybrid Homo sapiens
82 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
83 PRKCSH 5589
Co-fractionation Homo sapiens
84 TSEN34 79042
Co-fractionation Homo sapiens
85 SIDT2 51092
Affinity Capture-MS Homo sapiens
86 P2RX1 5023
Affinity Capture-MS Homo sapiens
87 EHHADH 1962
Two-hybrid Homo sapiens
88 BCKDHA 593
Co-fractionation Homo sapiens
89 RPAP2  
Affinity Capture-MS Homo sapiens
90 NDUFS7 374291
Co-fractionation Homo sapiens
91 PDHB 5162
Co-fractionation Homo sapiens
92 FAM25C  
Two-hybrid Homo sapiens
93 FAM25A  
Two-hybrid Homo sapiens
94 PTGER3  
Affinity Capture-MS Homo sapiens
95 ESR1  
Affinity Capture-MS Homo sapiens
96 NDUFS8 4728
Co-fractionation Homo sapiens
97 SEC62 7095
Affinity Capture-MS Homo sapiens
98 RAB7A 7879
Proximity Label-MS Homo sapiens
99 ATP1A1 476
Co-fractionation Homo sapiens
100 C1QBP 708
Co-fractionation Homo sapiens
101 MEA1  
Affinity Capture-MS Homo sapiens
102 CKAP4 10970
Co-fractionation Homo sapiens
103 ESYT1 23344
Co-fractionation Homo sapiens
View the network image/svg+xml



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