Experiment description of studies that identified CCNYL1 in sEVs

1 Experiment ID 1275 MISEV standards Biophysical techniques ✔ CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 1280 MISEV standards Biophysical techniques ✔ TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.151 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

4 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for CCNYL1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 DUSP22   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 GYPA   Affinity Capture-MS Homo sapiens 3 CIR1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 TCEAL2   Affinity Capture-MS Homo sapiens 5 BCAN   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 SERPINB4 6318 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 IVL 3713 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 HORMAD2   Affinity Capture-MS Homo sapiens 9 SPATA7 55812 Affinity Capture-MS Homo sapiens 10 OR1E1   Affinity Capture-MS Homo sapiens 11 HOXB5   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 YWHAE 7531 Affinity Capture-MS Homo sapiens 13 ARMC12   Affinity Capture-MS Homo sapiens 14 CDK16 5127 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 POF1B 79983 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 16 BIRC2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 FGB 2244 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 C19orf38   Affinity Capture-MS Homo sapiens 19 SERPINB2 5055 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 GRB10 2887 Affinity Capture-MS Homo sapiens 21 S100A7A 338324 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 PI4K2A 55361 Affinity Capture-MS Homo sapiens 23 DDX58 23586 Affinity Capture-RNA Homo sapiens 24 SERPINB5 5268 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 WEE1 7465 Affinity Capture-MS Homo sapiens 26 NXF1 10482 Affinity Capture-RNA Homo sapiens 27 A2ML1 144568 Affinity Capture-MS Homo sapiens 28 TREX2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 CERS2 29956 Affinity Capture-MS Homo sapiens 30 GJB7   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 PPL 5493 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 COMTD1 118881 Affinity Capture-MS Homo sapiens 33 FIS1 51024 Affinity Capture-MS Homo sapiens 34 MOV10 4343 Affinity Capture-RNA Homo sapiens 35 FGG 2266 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 SPPL2B 56928 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 CKAP5 9793 Affinity Capture-MS Homo sapiens 38 CENPM   Affinity Capture-MS Homo sapiens 39 S100A8 6279 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 RAB3IP   Affinity Capture-MS Homo sapiens 41 CAPG 822 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 MEOX2   Two-hybrid Homo sapiens 43 EVPL 2125 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 SBSN 374897 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 45 FYTTD1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 DHRS3 9249 Affinity Capture-MS Homo sapiens 47 TRIM29 23650 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 HIST2H3PS2 440686 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 CALML5 51806 Affinity Capture-MS Homo sapiens 50 CCNY 219771 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 51 TAS2R7   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 TACSTD2 4070 Affinity Capture-MS Homo sapiens 53 PKP3 11187 Affinity Capture-MS Homo sapiens 54 RTEL1 51750 Affinity Capture-MS Homo sapiens 55 TMEM185A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which CCNYL1 is involved

No pathways found