Gene description for Hspa1l
Gene name heat shock protein 1-like
Gene symbol Hspa1l
Other names/aliases Hsc70t
Msh5
Species Mus musculus
 Database cross references - Hspa1l
ExoCarta ExoCarta_15482
Vesiclepedia VP_15482
Entrez Gene 15482
UniProt P16627  
 Hspa1l identified in sEVs derived from the following tissue/cell type
Oligodendrocytes 21136642    
 Gene ontology annotations for Hspa1l
Molecular Function
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 IEA
    heat shock protein binding GO:0031072 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein folding chaperone GO:0044183 IBA
    unfolded protein binding GO:0051082 IEA
    unfolded protein binding GO:0051082 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    spermatogenesis GO:0007283 IEA
    binding of sperm to zona pellucida GO:0007339 IDA
    cell differentiation GO:0030154 IEA
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 IEA
    protein refolding GO:0042026 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    positive regulation of protein targeting to mitochondrion GO:1903955 IEA
    positive regulation of protein targeting to mitochondrion GO:1903955 ISO
Subcellular Localization
    zona pellucida receptor complex GO:0002199 IDA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    mitochondrial matrix GO:0005759 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    COP9 signalosome GO:0008180 IEA
    COP9 signalosome GO:0008180 ISO
    cell body GO:0044297 IDA
 Experiment description of studies that identified Hspa1l in sEVs
1
Experiment ID 33
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Hspa1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Proximity Label-MS Mus musculus
2 Mapt  
Affinity Capture-MS Mus musculus
3 Set 56086
Affinity Capture-MS Mus musculus
4 Rnf123  
Affinity Capture-MS Mus musculus
5 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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