Gene description for Hsph1
Gene name heat shock 105kDa/110kDa protein 1
Gene symbol Hsph1
Other names/aliases 105kDa
AI790491
Hsp105
Hsp110
hsp-E7I
hsp110/105
Species Mus musculus
 Database cross references - Hsph1
ExoCarta ExoCarta_15505
Vesiclepedia VP_15505
Entrez Gene 15505
UniProt Q61699  
 Hsph1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hsph1
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IBA
    adenyl-nucleotide exchange factor activity GO:0000774 ISO
    adenyl-nucleotide exchange factor activity GO:0000774 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    alpha-tubulin binding GO:0043014 IDA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IBA
    response to unfolded protein GO:0006986 TAS
    negative regulation of neuron apoptotic process GO:0043524 TAS
    positive regulation of MHC class I biosynthetic process GO:0045345 TAS
    chaperone cofactor-dependent protein refolding GO:0051085 IDA
    positive regulation of NK T cell activation GO:0051135 TAS
    regulation of microtubule cytoskeleton organization GO:0070507 NAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    microtubule GO:0005874 IDA
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Hsph1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hsph1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUSC2  
Affinity Capture-MS Homo sapiens
2 TCP11L1 55346
Affinity Capture-MS Homo sapiens
3 SNRPG 6637
Affinity Capture-MS Homo sapiens
4 SOD1 6647
Affinity Capture-MS Homo sapiens
5 BAZ1B 9031
Affinity Capture-MS Homo sapiens
6 EIF2S3 1968
Affinity Capture-MS Homo sapiens
7 MTNR1B  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
8 ANO3  
Affinity Capture-MS Homo sapiens
9 Kctd13  
Affinity Capture-MS Mus musculus
10 Chaf1a  
Affinity Capture-MS Mus musculus
11 Iqcb1  
Affinity Capture-MS Mus musculus
12 BUB3 9184
Affinity Capture-MS Homo sapiens
13 PLD1 5337
Affinity Capture-MS Homo sapiens
14 Tmem173  
Proximity Label-MS Mus musculus
15 Tuba1a 22142
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
16 Ranbp3  
Co-fractionation Mus musculus
17 BCL2L1 598
Affinity Capture-MS Homo sapiens
18 DNAJB4 11080
Affinity Capture-MS Homo sapiens
19 ZNFX1 57169
Affinity Capture-MS Homo sapiens
20 HSPA1A 3303
Affinity Capture-MS Homo sapiens
21 XPOT 11260
Affinity Capture-MS Homo sapiens
22 USMG5 84833
Affinity Capture-MS Homo sapiens
23 Eed  
Affinity Capture-MS Mus musculus
24 Mapt  
Affinity Capture-MS Mus musculus
25 DNAJB1 3337
Affinity Capture-MS Homo sapiens
26 USP9Y 8287
Affinity Capture-MS Homo sapiens
27 Fancd2  
Affinity Capture-MS Mus musculus
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