Gene description for Krt6a
Gene name keratin 6A
Gene symbol Krt6a
Other names/aliases 2310016L08Rik
Krt2-6a
Krt2-6c
MK6a
mK6[a]
Species Mus musculus
 Database cross references - Krt6a
ExoCarta ExoCarta_16687
Vesiclepedia VP_16687
Entrez Gene 16687
UniProt P50446  
 Krt6a identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Krt6a
Molecular Function
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    morphogenesis of an epithelium GO:0002009 IGI
    morphogenesis of an epithelium GO:0002009 IMP
    Wnt signaling pathway GO:0016055 IDA
    keratinization GO:0031424 IBA
    keratinization GO:0031424 IGI
    wound healing GO:0042060 IMP
    intermediate filament organization GO:0045109 IBA
    intermediate filament organization GO:0045109 IGI
    defense response to Gram-positive bacterium GO:0050830 IMP
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IMP
Subcellular Localization
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 IEA
 Experiment description of studies that identified Krt6a in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Krt6a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Golga4  
Co-fractionation Mus musculus
3 Nphp4  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Thrb  
Affinity Capture-MS Mus musculus
6 Dsg3  
Co-fractionation Mus musculus
7 MAPT  
Reconstituted Complex Homo sapiens
8 C4b  
Co-fractionation Mus musculus
9 Cenpe  
Co-fractionation Mus musculus
10 Zbtb4 75580
Co-fractionation Mus musculus
11 Ndc80  
Co-fractionation Mus musculus
12 Alb 11657
Co-fractionation Mus musculus
13 Mks1  
Affinity Capture-MS Mus musculus
14 Aga  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Krt6a is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Formation of the cornified envelope IEA Reactome
Keratinization IEA Reactome





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