Gene description for Alb
Gene name albumin
Gene symbol Alb
Other names/aliases Alb-1
Alb1
Species Mus musculus
 Database cross references - Alb
ExoCarta ExoCarta_11657
Vesiclepedia VP_11657
Entrez Gene 11657
UniProt P07724  
 Alb identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Fibroblasts 23260141    
Mast cells 17486113    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Alb
Molecular Function
    DNA binding GO:0003677 ISO
    DNA binding GO:0003677 ISS
    fatty acid binding GO:0005504 IEA
    fatty acid binding GO:0005504 ISO
    zinc ion binding GO:0008270 ISO
    toxic substance binding GO:0015643 ISO
    toxic substance binding GO:0015643 ISS
    oxygen binding GO:0019825 ISO
    oxygen binding GO:0019825 ISS
    enzyme binding GO:0019899 ISO
    pyridoxal phosphate binding GO:0030170 ISO
    pyridoxal phosphate binding GO:0030170 ISS
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    protein-folding chaperone binding GO:0051087 IEA
    protein-folding chaperone binding GO:0051087 ISO
    modified amino acid binding GO:0072341 ISO
    exogenous protein binding GO:0140272 IEA
    exogenous protein binding GO:0140272 ISO
    enterobactin binding GO:1903981 IBA
    enterobactin binding GO:1903981 ISO
    enterobactin binding GO:1903981 ISS
Biological Process
    cellular response to starvation GO:0009267 ISO
    cellular response to starvation GO:0009267 ISS
    positive regulation of circadian sleep/wake cycle, non-REM sleep GO:0046010 ISO
    negative regulation of mitochondrial depolarization GO:0051902 ISO
    negative regulation of mitochondrial depolarization GO:0051902 ISS
    cellular response to calcium ion starvation GO:0072732 ISO
    cellular response to calcium ion starvation GO:0072732 ISS
Subcellular Localization
    extracellular region GO:0005576 IDA
    basement membrane GO:0005604 ISO
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    protein-containing complex GO:0032991 ISO
    protein-containing complex GO:0032991 ISS
    myelin sheath GO:0043209 HDA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Alb in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
5
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Alb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Rnf123  
Affinity Capture-MS Mus musculus
3 Rbp4  
Co-fractionation Mus musculus
4 Tln2  
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 C4b  
Co-fractionation Mus musculus
8 Krt6a 16687
Co-fractionation Mus musculus
9 Serpina1b 20701
Co-fractionation Mus musculus
10 Set 56086
Affinity Capture-MS Mus musculus
11 Nphp1  
Affinity Capture-MS Mus musculus
12 Alb 11657
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
13 Qtrtd1  
Co-fractionation Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Cst3  
Co-fractionation Mus musculus
16 Kcnma1  
Affinity Capture-MS Mus musculus
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