Gene description for HBEGF
Gene name heparin-binding EGF-like growth factor
Gene symbol HBEGF
Other names/aliases DTR
DTS
DTSF
HEGFL
Species Homo sapiens
 Database cross references - HBEGF
ExoCarta ExoCarta_1839
Vesiclepedia VP_1839
Entrez Gene 1839
HGNC 3059
MIM 126150
UniProt Q99075  
 HBEGF identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for HBEGF
Molecular Function
    epidermal growth factor receptor binding GO:0005154 IBA
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IBA
    growth factor activity GO:0008083 IDA
    heparin binding GO:0008201 IBA
    heparin binding GO:0008201 IMP
    transmembrane receptor protein tyrosine kinase activator activity GO:0030297 IDA
    receptor ligand activity GO:0048018 IDA
Biological Process
    signal transduction GO:0007165 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 IMP
    muscle organ development GO:0007517 TAS
    regulation of heart contraction GO:0008016 IEA
    positive regulation of cell population proliferation GO:0008284 IBA
    positive regulation of cell growth GO:0030307 IEA
    positive regulation of cell migration GO:0030335 IMP
    wound healing, spreading of epidermal cells GO:0035313 IEA
    ERBB2-EGFR signaling pathway GO:0038134 IEA
    ERBB2-ERBB4 signaling pathway GO:0038135 IEA
    positive regulation of smooth muscle cell proliferation GO:0048661 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    negative regulation of elastin biosynthetic process GO:0051545 IEA
    positive regulation of keratinocyte migration GO:0051549 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IMP
    cell chemotaxis GO:0060326 IMP
    positive regulation of wound healing GO:0090303 IMP
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISS
    extracellular space GO:0005615 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    cell surface GO:0009986 NAS
    endocytic vesicle membrane GO:0030666 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
 Experiment description of studies that identified HBEGF in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HBEGF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 BCL6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 Bag1  
Reconstituted Complex Mus musculus
4 CD44 960
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
5 GPC1 2817
Affinity Capture-MS Homo sapiens
6 BAG1 573
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
7 MMP7 4316
Affinity Capture-Western Homo sapiens
8 GPC4 2239
Affinity Capture-MS Homo sapiens
9 NRD1 4898
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 EGFR 1956
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 OCLN 100506658
Affinity Capture-MS Homo sapiens
12 ADGRE5 976
Affinity Capture-MS Homo sapiens
13 ZBTB16  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
14 CD82 3732
Affinity Capture-Western Homo sapiens
15 NR3C1 2908
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HBEGF is involved
PathwayEvidenceSource
Bacterial Infection Pathways TAS Reactome
Cargo recognition for clathrin-mediated endocytosis TAS Reactome
Clathrin-mediated endocytosis TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downregulation of ERBB2 signaling TAS Reactome
EGFR downregulation TAS Reactome
EGFR interacts with phospholipase C-gamma TAS Reactome
EGFR Transactivation by Gastrin TAS Reactome
EGFR Transactivation by Gastrin IEA Reactome
ERBB2 Activates PTK6 Signaling TAS Reactome
ERBB2 Regulates Cell Motility TAS Reactome
ESR-mediated signaling TAS Reactome
Estrogen-dependent nuclear events downstream of ESR-membrane signaling TAS Reactome
Extra-nuclear estrogen signaling TAS Reactome
G alpha (q) signalling events TAS Reactome
G alpha (q) signalling events IEA Reactome
GAB1 signalosome TAS Reactome
Gastrin-CREB signalling pathway via PKC and MAPK TAS Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
GPCR downstream signalling TAS Reactome
GPCR downstream signalling IEA Reactome
GRB2 events in EGFR signaling TAS Reactome
GRB2 events in ERBB2 signaling TAS Reactome
Infectious disease TAS Reactome
Inhibition of Signaling by Overexpressed EGFR TAS Reactome
Intracellular signaling by second messengers TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Membrane Trafficking TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
Nuclear signaling by ERBB4 IEA Reactome
PI3K events in ERBB2 signaling TAS Reactome
PI3K events in ERBB4 signaling TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
PTK6 promotes HIF1A stabilization TAS Reactome
RAF/MAP kinase cascade TAS Reactome
SHC1 events in EGFR signaling TAS Reactome
SHC1 events in ERBB2 signaling IEA Reactome
SHC1 events in ERBB2 signaling TAS Reactome
SHC1 events in ERBB4 signaling TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by EGFR TAS Reactome
Signaling by EGFR in Cancer TAS Reactome
Signaling by ERBB2 TAS Reactome
Signaling by ERBB2 IEA Reactome
Signaling by ERBB2 in Cancer TAS Reactome
Signaling by ERBB2 KD Mutants TAS Reactome
Signaling by ERBB2 TMD/JMD mutants TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by ERBB4 IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by Non-Receptor Tyrosine Kinases TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by Overexpressed Wild-Type EGFR in Cancer TAS Reactome
Signaling by PTK6 TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Uptake and actions of bacterial toxins TAS Reactome
Uptake and function of diphtheria toxin TAS Reactome
Vesicle-mediated transport TAS Reactome





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