Gene description for ADGRE5
Gene name adhesion G protein-coupled receptor E5
Gene symbol ADGRE5
Other names/aliases CD97
TM7LN1
Species Homo sapiens
 Database cross references - ADGRE5
ExoCarta ExoCarta_976
Vesiclepedia VP_976
Entrez Gene 976
HGNC 1711
MIM 601211
UniProt P48960  
 ADGRE5 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 22740476    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for ADGRE5
Molecular Function
    transmembrane signaling receptor activity GO:0004888 TAS
    G protein-coupled receptor activity GO:0004930 IBA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    inflammatory response GO:0006954 TAS
    immune response GO:0006955 TAS
    cell adhesion GO:0007155 IEA
    cell surface receptor signaling pathway GO:0007166 IEA
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    cell-cell signaling GO:0007267 TAS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    membrane GO:0016020 HDA
    secretory granule membrane GO:0030667 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ADGRE5 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 201
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22740476    
Organism Homo sapiens
Experiment description Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors "Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ."
Journal name Electrophoresis
Publication year 2012
Sample Colorectal cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 282
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
14
Experiment ID 283
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 286
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
16
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
17
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 275
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
29
Experiment ID 274
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
30
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADGRE5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HMOX1 3162
Affinity Capture-MS Homo sapiens
2 SGCA  
Affinity Capture-MS Homo sapiens
3 SLC29A1 2030
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 C5AR1  
Affinity Capture-MS Homo sapiens
5 Cul1 26965
Affinity Capture-MS Mus musculus
6 OCLN 100506658
Affinity Capture-MS Homo sapiens
7 TMEM161A  
Affinity Capture-MS Homo sapiens
8 TTYH3 80727
Affinity Capture-MS Homo sapiens
9 SYNGR1 9145
Affinity Capture-MS Homo sapiens
10 KIAA2013 90231
Affinity Capture-MS Homo sapiens
11 JPH1 56704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 NDUFB5 4711
Affinity Capture-MS Homo sapiens
13 SURF4 6836
Affinity Capture-MS Homo sapiens
14 MFSD8 256471
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CMTM6 54918
Affinity Capture-MS Homo sapiens
16 TNFSF18 8995
Affinity Capture-MS Homo sapiens
17 POTEF 728378
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KIAA1429 25962
Affinity Capture-MS Homo sapiens
19 ARL6IP6  
Affinity Capture-MS Homo sapiens
20 PTDSS2 81490
Affinity Capture-MS Homo sapiens
21 MAOB  
Affinity Capture-MS Homo sapiens
22 TMEM252  
Affinity Capture-MS Homo sapiens
23 NDUFB8 4714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 P2RY1  
Affinity Capture-MS Homo sapiens
25 TMPPE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CASC5  
Affinity Capture-MS Homo sapiens
27 B4GALT7 11285
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TM9SF1 10548
Affinity Capture-MS Homo sapiens
29 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 GPRC5B 51704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 B3GAT3 26229
Affinity Capture-MS Homo sapiens
32 ADARB1 104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ZDHHC21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 IPO8 10526
Affinity Capture-MS Homo sapiens
35 ERMP1 79956
Affinity Capture-MS Homo sapiens
36 NDUFAF1  
Affinity Capture-MS Homo sapiens
37 KIAA0368 23392
Affinity Capture-MS Homo sapiens
38 PIGG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 BET1 10282
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
41 TMEM106A  
Affinity Capture-MS Homo sapiens
42 FZD7 8324
Affinity Capture-MS Homo sapiens
43 TMEM87A 25963
Affinity Capture-MS Homo sapiens
44 CLDND1 56650
Affinity Capture-MS Homo sapiens
45 ATP1A3 478
Affinity Capture-MS Homo sapiens
46 TMEM63B 55362
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 NNT 23530
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 GLT8D1 55830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
50 MTCH2 23788
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ALG6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 NDUFS4 4724
Affinity Capture-MS Homo sapiens
53 FUT8 2530
Affinity Capture-MS Homo sapiens
54 ND5 4540
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 XRN1 54464
Two-hybrid Homo sapiens
56 GPR35  
Affinity Capture-MS Homo sapiens
57 Srp72  
Affinity Capture-MS Mus musculus
58 BACE2 25825
Affinity Capture-MS Homo sapiens
59 LEMD2 221496
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 UPK1A 11045
Affinity Capture-MS Homo sapiens
62 ATP2C1 27032
Affinity Capture-MS Homo sapiens
63 XYLT2 64132
Affinity Capture-MS Homo sapiens
64 TMEM171  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 VDAC2 7417
Affinity Capture-MS Homo sapiens
66 ANKRD13A 88455
Affinity Capture-MS Homo sapiens
67 TMED1 11018
Affinity Capture-MS Homo sapiens
68 ZACN  
Affinity Capture-MS Homo sapiens
69 UPK2 7379
Affinity Capture-MS Homo sapiens
70 HNRNPD 3184
Two-hybrid Homo sapiens
71 VIPR2  
Affinity Capture-MS Homo sapiens
72 PSEN1 5663
Affinity Capture-MS Homo sapiens
73 TTYH1  
Affinity Capture-MS Homo sapiens
74 CANX 821
Affinity Capture-MS Homo sapiens
75 YIPF6 286451
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 NDUFA9 4704
Affinity Capture-MS Homo sapiens
77 LILRB4  
Affinity Capture-MS Homo sapiens
78 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 TMEM223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 GOLT1B 51026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 P2RY2 5029
Affinity Capture-MS Homo sapiens
82 CUL3 8452
Affinity Capture-MS Homo sapiens
83 EGFR 1956
Negative Genetic Homo sapiens
84 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 SERINC1 57515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 FDFT1 2222
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 CHRNB4  
Affinity Capture-MS Homo sapiens
88 RFT1 91869
Affinity Capture-MS Homo sapiens
89 NDUFB3 4709
Affinity Capture-MS Homo sapiens
90 GNB2 2783
Affinity Capture-MS Homo sapiens
91 ACKR2  
Affinity Capture-MS Homo sapiens
92 ZDHHC18 84243
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 SLC30A7 148867
Affinity Capture-MS Homo sapiens
94 ACAD9 28976
Affinity Capture-MS Homo sapiens
95 CERS6  
Affinity Capture-MS Homo sapiens
96 ENTPD2 954
Affinity Capture-MS Homo sapiens
97 CACNG1  
Affinity Capture-MS Homo sapiens
98 CYP2C9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 NTSR1  
Affinity Capture-MS Homo sapiens
100 FFAR1  
Affinity Capture-MS Homo sapiens
101 YIF1B 90522
Affinity Capture-MS Homo sapiens
102 SLC4A2 6522
Affinity Capture-MS Homo sapiens
103 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
104 GPR89A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 SYNGR2 9144
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 SERINC2 347735
Affinity Capture-MS Homo sapiens
107 PIGO  
Affinity Capture-MS Homo sapiens
108 OPALIN  
Affinity Capture-MS Homo sapiens
109 HBEGF 1839
Affinity Capture-MS Homo sapiens
110 EMR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 CLEC2D  
Affinity Capture-MS Homo sapiens
112 TMEM120B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 TSPAN15 23555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 B4GAT1 11041
Affinity Capture-MS Homo sapiens
115 UNC93B1 81622
Affinity Capture-MS Homo sapiens
116 SLC39A14 23516
Affinity Capture-MS Homo sapiens
117 ND4 4538
Affinity Capture-MS Homo sapiens
118 SCN4A  
Affinity Capture-MS Homo sapiens
119 GPRC5C 55890
Affinity Capture-MS Homo sapiens
120 NRAS 4893
Affinity Capture-MS Homo sapiens
121 TPST1 8460
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 GOLGA5 9950
Affinity Capture-MS Homo sapiens
123 EXTL3 2137
Affinity Capture-MS Homo sapiens
124 Samhd1 56045
Affinity Capture-MS Mus musculus
125 CLPTM1L 81037
Affinity Capture-MS Homo sapiens
126 SGCZ  
Affinity Capture-MS Homo sapiens
127 VDAC3 7419
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 SLC1A1 6505
Affinity Capture-MS Homo sapiens
129 OR1D4  
Affinity Capture-MS Homo sapiens
130 SCAMP3 10067
Affinity Capture-MS Homo sapiens
131 NAGPA 51172
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 ATP13A1 57130
Affinity Capture-MS Homo sapiens
133 ECSIT 51295
Affinity Capture-MS Homo sapiens
134 S1PR4  
Affinity Capture-MS Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here