Gene description for NAGPA
Gene name N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Gene symbol NAGPA
Other names/aliases APAA
UCE
Species Homo sapiens
 Database cross references - NAGPA
ExoCarta ExoCarta_51172
Vesiclepedia VP_51172
Entrez Gene 51172
HGNC 17378
MIM 607985
UniProt Q9UK23  
 NAGPA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NAGPA
Molecular Function
    N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity GO:0003944 IEA
    protein binding GO:0005515 IPI
Biological Process
    carbohydrate metabolic process GO:0005975 TAS
    protein glycosylation GO:0006486 IEA
    protein targeting to lysosome GO:0006622 TAS
    lysosome organization GO:0007040 TAS
    secretion of lysosomal enzymes GO:0033299 IBA
    protein modification process GO:0036211 TAS
Subcellular Localization
    membrane GO:0016020 TAS
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified NAGPA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NAGPA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NGLY1 55768
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CALR 811
Affinity Capture-MS Homo sapiens
3 CHRNA4  
Affinity Capture-MS Homo sapiens
4 SLAMF7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 FADS3 3995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RTN3 10313
Affinity Capture-MS Homo sapiens
7 WDR54  
Affinity Capture-MS Homo sapiens
8 EPS15 2060
Reconstituted Complex Homo sapiens
9 LGALS1 3956
Affinity Capture-MS Homo sapiens
10 TYW3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TMX1 81542
Affinity Capture-MS Homo sapiens
12 UBR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 FSHR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LRRC32 2615
Affinity Capture-MS Homo sapiens
15 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DEGS1  
Two-hybrid Homo sapiens
17 BDKRB1 623
Two-hybrid Homo sapiens
18 ZHX2  
Two-hybrid Homo sapiens
19 PCDHGA9  
Affinity Capture-MS Homo sapiens
20 AP2A1 160
Reconstituted Complex Homo sapiens
21 ADGRE5 976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TSEN15 116461
Affinity Capture-MS Homo sapiens
23 CCR8  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NAGPA is involved
No pathways found





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