Gene description for TMED1
Gene name transmembrane emp24 protein transport domain containing 1
Gene symbol TMED1
Other names/aliases IL1RL1LG
Il1rl1l
Tp24
Species Homo sapiens
 Database cross references - TMED1
ExoCarta ExoCarta_11018
Vesiclepedia VP_11018
Entrez Gene 11018
HGNC 17291
MIM 605395
UniProt Q13445  
 TMED1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TMED1
Molecular Function
    signaling receptor binding GO:0005102 TAS
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    Golgi organization GO:0007030 IBA
    signal transduction GO:0007165 TAS
    cell-cell signaling GO:0007267 TAS
    innate immune response GO:0045087 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IEA
    COPII-coated ER to Golgi transport vesicle GO:0030134 IBA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
 Experiment description of studies that identified TMED1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TMED1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 F2RL1  
Two-hybrid Homo sapiens
2 BFAR  
Affinity Capture-MS Homo sapiens
3 LIPG 9388
Affinity Capture-MS Homo sapiens
4 Tmed2 56334
Affinity Capture-MS Mus musculus
5 TPGS1  
Affinity Capture-MS Homo sapiens
6 TMED10 10972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 UBL4A 8266
Affinity Capture-MS Homo sapiens
8 TMED9 54732
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
9 CD226 10666
Affinity Capture-MS Homo sapiens
10 NKX2-5  
Affinity Capture-MS Homo sapiens
11 KIF14 9928
Affinity Capture-MS Homo sapiens
12 FBXL6  
Affinity Capture-MS Homo sapiens
13 Tmed10 68581
Affinity Capture-MS Mus musculus
14 KLK5 25818
Affinity Capture-MS Homo sapiens
15 EGLN3  
Affinity Capture-MS Homo sapiens
16 DUOXA2  
Affinity Capture-MS Homo sapiens
17 E2F4  
Affinity Capture-MS Homo sapiens
18 CHST8 64377
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SEC23B 10483
Protein-peptide Homo sapiens
20 TPTE  
Affinity Capture-MS Homo sapiens
21 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PLD4  
Affinity Capture-MS Homo sapiens
23 TP53 7157
Negative Genetic Homo sapiens
24 KLRD1  
Affinity Capture-MS Homo sapiens
25 FGF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 LTB4R2  
Two-hybrid Homo sapiens
27 PDE2A  
Affinity Capture-MS Homo sapiens
28 TSPAN2 10100
Affinity Capture-MS Homo sapiens
29 VAPA 9218
Affinity Capture-MS Homo sapiens
30 JUN 3725
Affinity Capture-MS Homo sapiens
31 RNF5  
Affinity Capture-MS Homo sapiens
32 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 S1PR1 1901
Affinity Capture-MS Homo sapiens
34 EXOC1 55763
Affinity Capture-MS Homo sapiens
35 EXOC3 11336
Affinity Capture-MS Homo sapiens
36 SMAD3 4088
Two-hybrid Homo sapiens
37 NFKBIL1  
Affinity Capture-MS Homo sapiens
38 CDK5RAP3 80279
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 IQCB1  
Affinity Capture-MS Homo sapiens
40 TPST2 8459
Affinity Capture-MS Homo sapiens
41 CREB3  
Affinity Capture-MS Homo sapiens
42 TMED2 10959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KCNK1  
Affinity Capture-MS Homo sapiens
44 CCDC101  
Affinity Capture-MS Homo sapiens
45 DDRGK1 65992
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ADGRE5 976
Affinity Capture-MS Homo sapiens
47 GPRC5B 51704
Affinity Capture-MS Homo sapiens
48 GPR37  
Two-hybrid Homo sapiens
49 FGF17  
Affinity Capture-MS Homo sapiens
50 RNF26  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
51 SAYSD1  
Affinity Capture-MS Homo sapiens
52 ATP2A3 489
Affinity Capture-MS Homo sapiens
53 GORASP2 26003
Affinity Capture-MS Homo sapiens
54 IL1RL1 9173
Reconstituted Complex Homo sapiens
55 S1PR4  
Affinity Capture-MS Homo sapiens
56 AVPR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMED1 is involved
No pathways found





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