Gene description for ND4
Gene name mitochondrially encoded NADH dehydrogenase 4
Gene symbol ND4
Other names/aliases MTND4
Species Homo sapiens
 Database cross references - ND4
ExoCarta ExoCarta_4538
Vesiclepedia VP_4538
Entrez Gene 4538
HGNC 7459
MIM 516003
UniProt P03905  
 ND4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ND4
Molecular Function
    NADH dehydrogenase activity GO:0003954 IBA
    NADH dehydrogenase (ubiquinone) activity GO:0008137 IMP
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
    ubiquinone binding GO:0048039 IBA
Biological Process
    response to hypoxia GO:0001666 IEA
    in utero embryonic development GO:0001701 IEA
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 IMP
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 IBA
    aerobic respiration GO:0009060 NAS
    electron transport coupled proton transport GO:0015990 IBA
    cerebellum development GO:0021549 IEA
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    response to nicotine GO:0035094 IEA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    response to ethanol GO:0045471 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified ND4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ND4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRELD1 78987
Affinity Capture-MS Homo sapiens
2 NDUFV3 4731
Affinity Capture-MS Homo sapiens
3 LIG4 3981
Two-hybrid Homo sapiens
4 GJA8  
Affinity Capture-MS Homo sapiens
5 NDUFA11 126328
Affinity Capture-MS Homo sapiens
6 DVL2 1856
Affinity Capture-MS Homo sapiens
7 ATP13A3 79572
Affinity Capture-MS Homo sapiens
8 TACR1  
Affinity Capture-MS Homo sapiens
9 GRB2 2885
Two-hybrid Homo sapiens
10 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NDUFA1 4694
Affinity Capture-MS Homo sapiens
12 ATP5J 522
Affinity Capture-MS Homo sapiens
13 NDUFA13 51079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 NDUFA9 4704
Affinity Capture-MS Homo sapiens
15 NIPAL1  
Affinity Capture-MS Homo sapiens
16 EXTL3 2137
Affinity Capture-MS Homo sapiens
17 NDUFB7 4713
Affinity Capture-MS Homo sapiens
18 APC  
Two-hybrid Homo sapiens
19 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NDUFS4 4724
Affinity Capture-MS Homo sapiens
21 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FUT1  
Affinity Capture-MS Homo sapiens
23 EMP3 2014
Affinity Capture-MS Homo sapiens
24 GHITM 27069
Affinity Capture-MS Homo sapiens
25 FFAR1  
Affinity Capture-MS Homo sapiens
26 LPAR1 1902
Affinity Capture-MS Homo sapiens
27 GPR35  
Affinity Capture-MS Homo sapiens
28 GOLGA7 51125
Affinity Capture-MS Homo sapiens
29 GPR17 2840
Affinity Capture-MS Homo sapiens
30 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 NDUFB3 4709
Affinity Capture-MS Homo sapiens
33 SPPL2B 56928
Affinity Capture-MS Homo sapiens
34 POMK  
Affinity Capture-MS Homo sapiens
35 NDUFA7 4701
Affinity Capture-MS Homo sapiens
36 TIMMDC1  
Affinity Capture-MS Homo sapiens
37 ASPH 444
Affinity Capture-MS Homo sapiens
38 ADGRE5 976
Affinity Capture-MS Homo sapiens
39 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
40 VIPR1  
Affinity Capture-MS Homo sapiens
41 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CCR6  
Affinity Capture-MS Homo sapiens
44 SLC22A4 6583
Affinity Capture-MS Homo sapiens
45 NDUFV2 4729
Affinity Capture-MS Homo sapiens
46 NDUFC2 4718
Affinity Capture-MS Homo sapiens
47 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FPR2  
Affinity Capture-MS Homo sapiens
49 MRPL18 29074
Affinity Capture-MS Homo sapiens
50 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ECSIT 51295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DNAJC30  
Affinity Capture-MS Homo sapiens
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