Gene description for Ptpra
Gene name protein tyrosine phosphatase, receptor type, A
Gene symbol Ptpra
Other names/aliases Ptpa
Ptpalpha
Rptpalpha
Rptra
Rptralpha
Species Mus musculus
 Database cross references - Ptpra
ExoCarta ExoCarta_19262
Vesiclepedia VP_19262
Entrez Gene 19262
UniProt P18052  
 Ptpra identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ptpra
Molecular Function
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 IMP
    protein tyrosine phosphatase activity GO:0004725 ISO
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    protein-containing complex binding GO:0044877 ISO
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    signal transduction GO:0007165 IBA
    integrin-mediated signaling pathway GO:0007229 IMP
    insulin receptor signaling pathway GO:0008286 IMP
    positive regulation of oligodendrocyte differentiation GO:0048714 ISO
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    regulation of focal adhesion assembly GO:0051893 IMP
Subcellular Localization
    focal adhesion GO:0005925 IMP
    membrane GO:0016020 IMP
    receptor complex GO:0043235 ISO
    synaptic membrane GO:0097060 IDA
    synaptic membrane GO:0097060 IEP
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
 Experiment description of studies that identified Ptpra in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ptpra
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp2r1a 51792
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Ptpn5  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
3 PTPRM 5797
Affinity Capture-Western Homo sapiens
4 Ptprf  
Co-fractionation Mus musculus
5 Ptpn6 15170
Co-fractionation Mus musculus
6 Ptpn3  
Co-fractionation Mus musculus
7 Ptprk 19272
Co-fractionation Mus musculus
8 Eif3g 53356
Co-fractionation Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 Ptpre  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 Fyn  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
12 Ptprc 19264
Affinity Capture-Western Mus musculus
13 PTPRF 5792
Affinity Capture-Western Homo sapiens
14 Ptprm  
Co-fractionation Mus musculus
15 Ptprz1  
Co-fractionation Mus musculus
16 Uvrag  
Co-fractionation Mus musculus
17 Ptprq  
Co-fractionation Mus musculus
18 Ptprs 19280
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
19 FYN 2534
Biochemical Activity Homo sapiens
20 Ptpru  
Co-fractionation Mus musculus
21 1700021K19Rik  
Co-fractionation Mus musculus
22 Sstr3  
Proximity Label-MS Mus musculus
23 Ptprd  
Affinity Capture-Western Mus musculus
24 Ptpn13  
Co-fractionation Mus musculus
25 Tgfbrap1  
Co-fractionation Mus musculus
26 Ptpn11 19247
Co-fractionation Mus musculus
27 Ptprn2  
Co-fractionation Mus musculus
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