Gene description for Eif3g
Gene name eukaryotic translation initiation factor 3, subunit G
Gene symbol Eif3g
Other names/aliases 44kDa
D0Jmb4
Eif3s4
TU-189B2
eIF3-p44
p44
Species Mus musculus
 Database cross references - Eif3g
ExoCarta ExoCarta_53356
Vesiclepedia VP_53356
Entrez Gene 53356
UniProt Q9Z1D1  
 Eif3g identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif3g
Molecular Function
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 ISO
    protein binding GO:0005515 IPI
Biological Process
    formation of cytoplasmic translation initiation complex GO:0001732 IEA
    translational initiation GO:0006413 IDA
    translational initiation GO:0006413 ISO
    viral translational termination-reinitiation GO:0075525 IEA
    viral translational termination-reinitiation GO:0075525 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified Eif3g in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif3g
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GTSE1 51512
Two-hybrid Homo sapiens
2 EXOSC7 23016
Two-hybrid Homo sapiens
3 Pik3c3  
Co-fractionation Mus musculus
4 SARS 6301
Two-hybrid Homo sapiens
5 TFIP11  
Two-hybrid Homo sapiens
6 Ptpra 19262
Co-fractionation Mus musculus
7 Mrpl13  
Two-hybrid Mus musculus
8 MED1 5469
Two-hybrid Homo sapiens
9 Pkn2  
Co-fractionation Mus musculus
10 PRPF6 24148
Two-hybrid Homo sapiens
11 Cnn3 71994
Two-hybrid Mus musculus
12 Eif1 20918
Co-fractionation Mus musculus
13 HOOK3 84376
Two-hybrid Homo sapiens
14 EIF4A3 9775
Two-hybrid Homo sapiens
15 NXF1 10482
Two-hybrid Homo sapiens
16 Mettl21e  
Affinity Capture-MS Mus musculus
17 Chaf1a  
Affinity Capture-MS Mus musculus
18 EIF4ENIF1 56478
Two-hybrid Homo sapiens
19 Ranbp2  
Co-fractionation Mus musculus
20 PRC1 9055
Two-hybrid Homo sapiens
21 CPSF7 79869
Two-hybrid Homo sapiens
22 Eed  
Affinity Capture-MS Mus musculus
23 Paip1  
Affinity Capture-Western Mus musculus
24 TEX10 54881
Two-hybrid Homo sapiens
25 TXNL4A 10907
Two-hybrid Homo sapiens
26 Tcf3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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