Gene description for Hspa1a
Gene name heat shock protein 1A
Gene symbol Hspa1a
Other names/aliases Hsp70-3
Hsp70.3
Hsp72
hsp68
hsp70A1
Species Mus musculus
 Database cross references - Hspa1a
ExoCarta ExoCarta_193740
Vesiclepedia VP_193740
Entrez Gene 193740
UniProt Q61696  
 Hspa1a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Oligodendrocytes 21136642    
Osteosarcoma cells 33785738    
Osteosarcoma cells 33785738    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hspa1a
Molecular Function
    G protein-coupled receptor binding GO:0001664 ISO
    protease binding GO:0002020 ISO
    transcription corepressor activity GO:0003714 ISO
    transcription corepressor activity GO:0003714 ISS
    double-stranded RNA binding GO:0003725 ISO
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 ISO
    enzyme binding GO:0019899 ISO
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 ISO
    denatured protein binding GO:0031249 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    death receptor agonist activity GO:0038177 ISO
    histone deacetylase binding GO:0042826 ISO
    protein folding chaperone GO:0044183 IBA
    protein folding chaperone GO:0044183 IDA
    protein folding chaperone GO:0044183 ISO
    receptor ligand activity GO:0048018 ISO
    NF-kappaB binding GO:0051059 ISO
    unfolded protein binding GO:0051082 ISO
    misfolded protein binding GO:0051787 ISO
    C3HC4-type RING finger domain binding GO:0055131 ISO
    disordered domain specific binding GO:0097718 ISO
    ATP-dependent protein disaggregase activity GO:0140545 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    telomere maintenance GO:0000723 IMP
    DNA repair GO:0006281 IMP
    mRNA catabolic process GO:0006402 ISS
    protein folding GO:0006457 IDA
    response to unfolded protein GO:0006986 ISS
    lysosomal transport GO:0007041 IMP
    negative regulation of cell population proliferation GO:0008285 ISS
    response to heat GO:0009408 IDA
    response to heat GO:0009408 IMP
    negative regulation of cell growth GO:0030308 ISS
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IBA
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 ISS
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    positive regulation of microtubule nucleation GO:0090063 ISS
    regulation of mitotic spindle assembly GO:1901673 ISS
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 ISS
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    COP9 signalosome GO:0008180 ISO
    inclusion body GO:0016234 ISO
    aggresome GO:0016235 ISO
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    protein-containing complex GO:0032991 ISO
    membrane raft GO:0045121 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Hspa1a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 1321
MISEV standards
Biophysical techniques
✘ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33785738    
Organism Homo sapiens
Experiment description Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
Authors "Todkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M. "
Journal name Nat Commun
Publication year 2021
Sample Osteosarcoma cells
Sample name U2OS
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 1322
MISEV standards
Biophysical techniques
✘ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33785738    
Organism Homo sapiens
Experiment description Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs
Authors "Todkar K, Chikhi L, Desjardins V, El-Mortada F, Pépin G, Germain M. "
Journal name Nat Commun
Publication year 2021
Sample Osteosarcoma cells
Sample name U2OS
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hspa1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
2 Dnajc7 56354
Co-fractionation Mus musculus
3 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Tubgcp2  
Co-fractionation Mus musculus
6 Mapt  
Affinity Capture-MS Mus musculus
7 Atg5  
Affinity Capture-RNA Mus musculus
8 Nqo1  
Co-fractionation Mus musculus
9 Park2  
Affinity Capture-Western Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
11 Dnajc3  
Co-fractionation Mus musculus
12 Tomm70a  
Co-fractionation Mus musculus
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