Gene description for Got2
Gene name glutamatic-oxaloacetic transaminase 2, mitochondrial
Gene symbol Got2
Other names/aliases AL022787
FABP-pm
Got-2
mAspAT
Species Mus musculus
 Database cross references - Got2
ExoCarta ExoCarta_14719
Vesiclepedia VP_14719
Entrez Gene 14719
UniProt P05202  
 Got2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Got2
Molecular Function
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 IBA
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 IDA
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 ISO
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 ISS
    phospholipid binding GO:0005543 ISO
    kynurenine-oxoglutarate transaminase activity GO:0016212 IEA
    amino acid binding GO:0016597 ISO
    enzyme binding GO:0019899 ISO
    pyridoxal phosphate binding GO:0030170 IEA
    pyridoxal phosphate binding GO:0030170 ISO
    carboxylic acid binding GO:0031406 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    2-oxoglutarate metabolic process GO:0006103 ISS
    oxaloacetate metabolic process GO:0006107 IDA
    oxaloacetate metabolic process GO:0006107 ISO
    amino acid metabolic process GO:0006520 ISO
    aspartate metabolic process GO:0006531 ISO
    aspartate metabolic process GO:0006531 ISS
    aspartate biosynthetic process GO:0006532 IDA
    aspartate catabolic process GO:0006533 IBA
    aspartate catabolic process GO:0006533 IEA
    aspartate catabolic process GO:0006533 ISO
    glutamate metabolic process GO:0006536 ISS
    glutamate catabolic process GO:0006538 IDA
    fatty acid transport GO:0015908 IEA
    glutamate catabolic process to aspartate GO:0019550 IDA
    malate-aspartate shuttle GO:0043490 IMP
    dicarboxylic acid metabolic process GO:0043648 ISO
    response to ethanol GO:0045471 IEA
    response to ethanol GO:0045471 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial matrix GO:0005759 IDA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 ISO
    T-tubule GO:0030315 ISO
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 ISO
    perikaryon GO:0043204 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Got2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Got2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hspa1a 193740
Cross-Linking-MS (XL-MS) Mus musculus
2 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Cox4i1 12857
Cross-Linking-MS (XL-MS) Mus musculus
5 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
6 Slc25a3 18674
Cross-Linking-MS (XL-MS) Mus musculus
7 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
9 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
10 Hadh  
Cross-Linking-MS (XL-MS) Mus musculus
11 Acadl 11363
Cross-Linking-MS (XL-MS) Mus musculus
12 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
13 Acadvl 11370
Cross-Linking-MS (XL-MS) Mus musculus
14 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
15 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
16 Ech1  
Cross-Linking-MS (XL-MS) Mus musculus
17 Aldh4a1  
Cross-Linking-MS (XL-MS) Mus musculus
18 Cox7a1  
Cross-Linking-MS (XL-MS) Mus musculus
19 Sdhb 67680
Co-fractionation Mus musculus
20 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
21 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
22 Abcd2  
Cross-Linking-MS (XL-MS) Mus musculus
23 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
24 MAPT  
Reconstituted Complex Homo sapiens
25 Idh3a 67834
Cross-Linking-MS (XL-MS) Mus musculus
26 Acadm 11364
Cross-Linking-MS (XL-MS) Mus musculus
27 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
28 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
29 Gfm2  
Cross-Linking-MS (XL-MS) Mus musculus
30 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
31 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
32 Ndufa12  
Cross-Linking-MS (XL-MS) Mus musculus
33 Eci1  
Cross-Linking-MS (XL-MS) Mus musculus
34 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
35 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
36 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
37 Uqcrc1  
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
38 Uqcrc2 67003
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
39 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
40 Idh3g  
Co-fractionation Mus musculus
41 Acot13  
Cross-Linking-MS (XL-MS) Mus musculus
42 Acaa2  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here