Gene description for Slc25a3
Gene name solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3
Gene symbol Slc25a3
Other names/aliases 5730556H19Rik
PTP
Phc
Species Mus musculus
 Database cross references - Slc25a3
ExoCarta ExoCarta_18674
Vesiclepedia VP_18674
Entrez Gene 18674
UniProt Q8VEM8  
 Slc25a3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc25a3
Molecular Function
    phosphate transmembrane transporter activity GO:0005315 IBA
    phosphate transmembrane transporter activity GO:0005315 IEA
    phosphate:proton symporter activity GO:0015317 ISS
    protein-containing complex binding GO:0044877 ISO
Biological Process
    phosphate ion transmembrane transport GO:0035435 IBA
    proton transmembrane transport GO:1902600 IEA
    mitochondrial phosphate ion transmembrane transport GO:1990547 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 IBA
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Slc25a3 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc25a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ict1  
Co-fractionation Mus musculus
2 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
3 Cox4i1 12857
Co-fractionation Mus musculus
4 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
5 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
6 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
7 Slc25a3 18674
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Gbas  
Cross-Linking-MS (XL-MS) Mus musculus
9 Uqcrh  
Cross-Linking-MS (XL-MS) Mus musculus
10 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
11 Kcna3  
Affinity Capture-MS Mus musculus
12 Kctd13  
Affinity Capture-MS Mus musculus
13 Htt  
Affinity Capture-MS Mus musculus
14 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
15 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
16 SNCA 6622
Affinity Capture-MS Homo sapiens
17 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
18 Unk  
Affinity Capture-RNA Mus musculus
19 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
20 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
21 Sod2 20656
Co-fractionation Mus musculus
22 Sqstm1  
Affinity Capture-MS Mus musculus
23 Eed  
Affinity Capture-MS Mus musculus
24 C1qbp 12261
Co-fractionation Mus musculus
25 Mapt  
Affinity Capture-MS Mus musculus
26 Atg16l1  
Affinity Capture-MS Mus musculus
27 Uqcrb  
Cross-Linking-MS (XL-MS) Mus musculus
28 Fancd2  
Affinity Capture-MS Mus musculus
29 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
30 Acadvl 11370
Cross-Linking-MS (XL-MS) Mus musculus
31 Lipc  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Slc25a3 is involved
No pathways found





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