Gene description for Sema3a
Gene name sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Gene symbol Sema3a
Other names/aliases Hsema-I
SEMA1
SemD
Semad
coll-1
Species Mus musculus
 Database cross references - Sema3a
ExoCarta ExoCarta_20346
Vesiclepedia VP_20346
Entrez Gene 20346
UniProt O08665  
 Sema3a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sema3a
Molecular Function
    protein binding GO:0005515 IPI
    semaphorin receptor binding GO:0030215 IBA
    semaphorin receptor binding GO:0030215 IPI
    neuropilin binding GO:0038191 IBA
    neuropilin binding GO:0038191 IPI
    chemorepellent activity GO:0045499 IBA
    chemorepellent activity GO:0045499 IDA
Biological Process
    neural crest cell migration GO:0001755 IBA
    neuron migration GO:0001764 IMP
    regulation of heart rate GO:0002027 IMP
    nervous system development GO:0007399 IGI
    axon guidance GO:0007411 IDA
    axon guidance GO:0007411 IMP
    axon guidance GO:0007411 ISO
    axonal fasciculation GO:0007413 IMP
    synaptic target recognition GO:0008039 IDA
    synaptic target recognition GO:0008039 IMP
    motor neuron axon guidance GO:0008045 IBA
    motor neuron axon guidance GO:0008045 IMP
    epithelial cell migration GO:0010631 IDA
    negative regulation of epithelial cell migration GO:0010633 IDA
    negative regulation of neuron projection development GO:0010977 ISO
    facial nerve structural organization GO:0021612 IMP
    trigeminal nerve structural organization GO:0021637 IMP
    nerve development GO:0021675 IMP
    olfactory bulb development GO:0021772 ISO
    olfactory bulb development GO:0021772 ISS
    branchiomotor neuron axon guidance GO:0021785 IMP
    gonadotrophin-releasing hormone neuronal migration to the hypothalamus GO:0021828 IMP
    positive regulation of cell migration GO:0030335 IBA
    negative regulation of axon extension GO:0030517 IDA
    negative regulation of axon extension GO:0030517 IGI
    negative regulation of axon extension GO:0030517 ISO
    ventral trunk neural crest cell migration GO:0036486 IMP
    axon extension GO:0048675 IDA
    axon extension GO:0048675 IGI
    dendrite morphogenesis GO:0048813 IMP
    regulation of axon extension involved in axon guidance GO:0048841 IDA
    regulation of axon extension involved in axon guidance GO:0048841 ISO
    negative regulation of axon extension involved in axon guidance GO:0048843 IDA
    negative regulation of axon extension involved in axon guidance GO:0048843 IGI
    axon extension involved in axon guidance GO:0048846 IDA
    axon extension involved in axon guidance GO:0048846 IGI
    axon extension involved in axon guidance GO:0048846 IMP
    negative chemotaxis GO:0050919 IBA
    negative chemotaxis GO:0050919 IGI
    axonogenesis involved in innervation GO:0060385 IMP
    dichotomous subdivision of terminal units involved in salivary gland branching GO:0060666 IDA
    dichotomous subdivision of terminal units involved in salivary gland branching GO:0060666 IGI
    sympathetic ganglion development GO:0061549 IMP
    trigeminal ganglion development GO:0061551 IMP
    semaphorin-plexin signaling pathway GO:0071526 IBA
    semaphorin-plexin signaling pathway GO:0071526 IGI
    semaphorin-plexin signaling pathway GO:0071526 IMP
    sympathetic neuron projection extension GO:0097490 IMP
    sympathetic neuron projection guidance GO:0097491 IMP
    maintenance of synapse structure GO:0099558 IDA
    maintenance of synapse structure GO:0099558 IMP
    basal dendrite arborization GO:0150020 IGI
    neural crest cell migration involved in autonomic nervous system development GO:1901166 IMP
    neural crest cell migration involved in sympathetic nervous system development GO:1903045 IMP
    facioacoustic ganglion development GO:1903375 IMP
    positive regulation of male gonad development GO:2000020 IMP
    positive regulation of neuron migration GO:2001224 IGI
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    plasma membrane GO:0005886 IBA
    axon GO:0030424 IBA
    axon GO:0030424 ISO
    dendrite GO:0030425 ISO
    glutamatergic synapse GO:0098978 IBA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    cerebellar climbing fiber to Purkinje cell synapse GO:0150053 IDA
    cerebellar climbing fiber to Purkinje cell synapse GO:0150053 IMP
 Experiment description of studies that identified Sema3a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sema3a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Npr1  
Reconstituted Complex Mus musculus
2 Nrp1 18186
Reconstituted Complex Mus musculus
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