Gene description for Nrp1
Gene name neuropilin 1
Gene symbol Nrp1
Other names/aliases C530029I03
NP-1
NPN-1
Npn1
Nrp
Species Mus musculus
 Database cross references - Nrp1
ExoCarta ExoCarta_18186
Vesiclepedia VP_18186
Entrez Gene 18186
UniProt P97333  
 Nrp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nrp1
Molecular Function
    vascular endothelial growth factor receptor activity GO:0005021 IBA
    vascular endothelial growth factor receptor activity GO:0005021 IMP
    GTPase activator activity GO:0005096 IEA
    GTPase activator activity GO:0005096 ISO
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IEA
    semaphorin receptor activity GO:0017154 IBA
    semaphorin receptor activity GO:0017154 IDA
    semaphorin receptor activity GO:0017154 IGI
    semaphorin receptor activity GO:0017154 IPI
    semaphorin receptor activity GO:0017154 ISO
    growth factor binding GO:0019838 IPI
    growth factor binding GO:0019838 ISO
    protein kinase binding GO:0019901 IPI
    vascular endothelial growth factor binding GO:0038085 IBA
    vascular endothelial growth factor binding GO:0038085 IPI
    vascular endothelial growth factor binding GO:0038085 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    angiogenesis GO:0001525 IGI
    angiogenesis GO:0001525 IMP
    angiogenesis GO:0001525 IMP
    angiogenesis GO:0001525 ISO
    branching involved in blood vessel morphogenesis GO:0001569 IMP
    branching involved in blood vessel morphogenesis GO:0001569 IMP
    vasculogenesis GO:0001570 IBA
    neural crest cell migration GO:0001755 IBA
    neuron migration GO:0001764 IMP
    sprouting angiogenesis GO:0002040 IBA
    sprouting angiogenesis GO:0002040 IMP
    cell migration involved in sprouting angiogenesis GO:0002042 IC
    cell migration involved in sprouting angiogenesis GO:0002042 IMP
    outflow tract septum morphogenesis GO:0003148 IMP
    cardiac neural crest cell migration involved in outflow tract morphogenesis GO:0003253 IMP
    substrate-dependent cell migration, cell extension GO:0006930 IEA
    substrate-dependent cell migration, cell extension GO:0006930 ISO
    integrin-mediated signaling pathway GO:0007229 IMP
    integrin-mediated signaling pathway GO:0007229 ISO
    axon guidance GO:0007411 IBA
    axon guidance GO:0007411 IDA
    axon guidance GO:0007411 IMP
    axonal fasciculation GO:0007413 IMP
    heart development GO:0007507 IGI
    motor neuron axon guidance GO:0008045 IMP
    response to wounding GO:0009611 IBA
    positive regulation of endothelial cell migration GO:0010595 IBA
    positive regulation of endothelial cell migration GO:0010595 IMP
    positive regulation of endothelial cell migration GO:0010595 ISO
    positive regulation of platelet-derived growth factor receptor signaling pathway GO:0010641 IEA
    positive regulation of platelet-derived growth factor receptor signaling pathway GO:0010641 ISO
    dendrite development GO:0016358 IMP
    facial nerve structural organization GO:0021612 IMP
    trigeminal nerve morphogenesis GO:0021636 IMP
    trigeminal nerve structural organization GO:0021637 IMP
    vestibulocochlear nerve structural organization GO:0021649 IMP
    branchiomotor neuron axon guidance GO:0021785 IMP
    gonadotrophin-releasing hormone neuronal migration to the hypothalamus GO:0021828 IMP
    negative regulation of axon extension GO:0030517 IGI
    regulation of vascular endothelial growth factor receptor signaling pathway GO:0030947 IBA
    retinal ganglion cell axon guidance GO:0031290 IMP
    regulation of Cdc42 protein signal transduction GO:0032489 IMP
    regulation of Cdc42 protein signal transduction GO:0032489 ISO
    cellular response to hepatocyte growth factor stimulus GO:0035729 IEA
    cellular response to hepatocyte growth factor stimulus GO:0035729 ISO
    endothelial cell chemotaxis GO:0035767 IEA
    endothelial cell chemotaxis GO:0035767 ISO
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IMP
    ventral trunk neural crest cell migration GO:0036486 IMP
    neuropilin signaling pathway GO:0038189 IMP
    neuropilin signaling pathway GO:0038189 ISO
    VEGF-activated neuropilin signaling pathway GO:0038190 IMP
    VEGF-activated neuropilin signaling pathway GO:0038190 ISO
    wound healing GO:0042060 IEA
    wound healing GO:0042060 ISO
    positive regulation of phosphorylation GO:0042327 IMP
    negative regulation of neuron apoptotic process GO:0043524 IMP
    blood vessel endothelial cell migration GO:0043534 IMP
    endothelial cell migration GO:0043542 IMP
    positive regulation of angiogenesis GO:0045766 IMP
    symbiont entry into host cell GO:0046718 IEA
    symbiont entry into host cell GO:0046718 ISO
    platelet-derived growth factor receptor signaling pathway GO:0048008 IEA
    platelet-derived growth factor receptor signaling pathway GO:0048008 ISO
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IBA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 ISO
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 ISO
    sympathetic nervous system development GO:0048485 IMP
    neuron development GO:0048666 IMP
    axon extension GO:0048675 IGI
    regulation of axon extension involved in axon guidance GO:0048841 IDA
    positive regulation of axon extension involved in axon guidance GO:0048842 IMP
    negative regulation of axon extension involved in axon guidance GO:0048843 IDA
    negative regulation of axon extension involved in axon guidance GO:0048843 ISO
    artery morphogenesis GO:0048844 IMP
    axon extension involved in axon guidance GO:0048846 IDA
    axon extension involved in axon guidance GO:0048846 IMP
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IMP
    positive chemotaxis GO:0050918 IMP
    positive regulation of filopodium assembly GO:0051491 IBA
    positive regulation of filopodium assembly GO:0051491 IMP
    positive regulation of filopodium assembly GO:0051491 ISO
    positive regulation of stress fiber assembly GO:0051496 IEA
    positive regulation of stress fiber assembly GO:0051496 ISO
    positive regulation of focal adhesion assembly GO:0051894 IEA
    positive regulation of focal adhesion assembly GO:0051894 ISO
    axonogenesis involved in innervation GO:0060385 IMP
    dichotomous subdivision of terminal units involved in salivary gland branching GO:0060666 IGI
    dichotomous subdivision of terminal units involved in salivary gland branching GO:0060666 IMP
    angiogenesis involved in coronary vascular morphogenesis GO:0060978 IMP
    cell migration involved in coronary vasculogenesis GO:0060980 IC
    coronary artery morphogenesis GO:0060982 IMP
    retina vasculature development in camera-type eye GO:0061298 IMP
    retina vasculature morphogenesis in camera-type eye GO:0061299 IMP
    renal artery morphogenesis GO:0061441 IMP
    sympathetic ganglion development GO:0061549 IMP
    trigeminal ganglion development GO:0061551 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    semaphorin-plexin signaling pathway GO:0071526 IDA
    semaphorin-plexin signaling pathway GO:0071526 IGI
    semaphorin-plexin signaling pathway GO:0071526 IMP
    positive regulation of smooth muscle cell chemotaxis GO:0071673 IEA
    positive regulation of smooth muscle cell chemotaxis GO:0071673 ISO
    commissural neuron axon guidance GO:0071679 IMP
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 IMP
    sensory neuron axon guidance GO:0097374 IMP
    motor neuron migration GO:0097475 IMP
    sympathetic neuron projection extension GO:0097490 IMP
    sympathetic neuron projection guidance GO:0097491 IMP
    postsynapse organization GO:0099173 IDA
    postsynapse organization GO:0099173 IMP
    basal dendrite development GO:0150018 IMP
    basal dendrite arborization GO:0150020 IGI
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    neural crest cell migration involved in autonomic nervous system development GO:1901166 IMP
    protein localization to early endosome GO:1902946 IMP
    facioacoustic ganglion development GO:1903375 IMP
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 IEA
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 ISO
    dorsal root ganglion morphogenesis GO:1904835 IMP
    otic placode development GO:1905040 IMP
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
Subcellular Localization
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    neurofilament GO:0005883 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 IDA
    focal adhesion GO:0005925 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    axon GO:0030424 IBA
    axon GO:0030424 IDA
    axon GO:0030424 ISO
    growth cone GO:0030426 IEA
    growth cone GO:0030426 ISO
    mitochondrial membrane GO:0031966 IEA
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    postsynaptic membrane GO:0045211 IDA
    sorting endosome GO:0097443 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Nrp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nrp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEMA3F 6405
Reconstituted Complex Homo sapiens
2 Plxnd1  
Affinity Capture-Western Mus musculus
3 Sema3a 20346
Reconstituted Complex Mus musculus
4 Plxna1 18844
Affinity Capture-Western Mus musculus
5 Plxna2  
Reconstituted Complex Mus musculus
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