Gene description for ETFDH
Gene name electron-transferring-flavoprotein dehydrogenase
Gene symbol ETFDH
Other names/aliases ETFQO
MADD
Species Homo sapiens
 Database cross references - ETFDH
ExoCarta ExoCarta_2110
Vesiclepedia VP_2110
Entrez Gene 2110
HGNC 3483
MIM 231675
UniProt Q16134  
 ETFDH identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ETFDH
Molecular Function
    electron-transferring-flavoprotein dehydrogenase activity GO:0004174 IBA
    electron-transferring-flavoprotein dehydrogenase activity GO:0004174 IDA
    electron-transferring-flavoprotein dehydrogenase activity GO:0004174 TAS
    protein binding GO:0005515 IPI
    electron transfer activity GO:0009055 IDA
    oxidoreductase activity GO:0016491 IDA
    metal ion binding GO:0046872 IEA
    quinone binding GO:0048038 IDA
    ubiquinone binding GO:0048039 IDA
    flavin adenine dinucleotide binding GO:0050660 ISS
    4 iron, 4 sulfur cluster binding GO:0051539 IDA
Biological Process
    response to oxidative stress GO:0006979 ISS
    electron transport chain GO:0022900 IBA
    electron transport chain GO:0022900 IDA
    respiratory electron transport chain GO:0022904 TAS
    fatty acid beta-oxidation using acyl-CoA dehydrogenase GO:0033539 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial membrane GO:0031966 IDA
 Experiment description of studies that identified ETFDH in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ETFDH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Co-fractionation Homo sapiens
2 TPP1 1200
Co-fractionation Homo sapiens
3 NXF1 10482
Affinity Capture-RNA Homo sapiens
4 LRPPRC 10128
Co-fractionation Homo sapiens
5 HBB 3043
Affinity Capture-MS Homo sapiens
6 ZNF581  
Two-hybrid Homo sapiens
7 TRIM69 140691
Two-hybrid Homo sapiens
8 MOV10 4343
Affinity Capture-RNA Homo sapiens
9 KRTAP11-1  
Two-hybrid Homo sapiens
10 HSD17B10 3028
Two-hybrid Homo sapiens
11 CANX 821
Co-fractionation Homo sapiens
12 COQ9  
Affinity Capture-MS Homo sapiens
13 BCKDHB 594
Co-fractionation Homo sapiens
14 OTX1  
Two-hybrid Homo sapiens
15 KRTAP13-2  
Two-hybrid Homo sapiens
16 VCP 7415
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which ETFDH is involved
PathwayEvidenceSource
Aerobic respiration and respiratory electron transport TAS Reactome
Metabolism TAS Reactome
Respiratory electron transport TAS Reactome





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