Gene description for BCKDHB
Gene name branched chain keto acid dehydrogenase E1, beta polypeptide
Gene symbol BCKDHB
Other names/aliases E1B
dJ279A18.1
Species Homo sapiens
 Database cross references - BCKDHB
ExoCarta ExoCarta_594
Vesiclepedia VP_594
Entrez Gene 594
HGNC 987
MIM 248611
UniProt P21953  
 BCKDHB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for BCKDHB
Molecular Function
    3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity GO:0003863 IDA
    3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity GO:0003863 IMP
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IEA
    branched-chain alpha-keto acid dehydrogenase activity GO:0047101 IDA
Biological Process
    lipid metabolic process GO:0006629 IEA
    response to nutrient GO:0007584 IBA
    branched-chain amino acid catabolic process GO:0009083 IBA
    branched-chain amino acid catabolic process GO:0009083 IDA
    branched-chain amino acid catabolic process GO:0009083 IMP
    response to glucocorticoid GO:0051384 IEA
    response to cAMP GO:0051591 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IMP
    mitochondrial matrix GO:0005759 NAS
    mitochondrial matrix GO:0005759 TAS
    oxoglutarate dehydrogenase complex GO:0045252 IBA
    branched-chain alpha-ketoacid dehydrogenase complex GO:0160157 IDA
    branched-chain alpha-ketoacid dehydrogenase complex GO:0160157 IMP
    branched-chain alpha-ketoacid dehydrogenase complex GO:0160157 IPI
 Experiment description of studies that identified BCKDHB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BCKDHB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFV1 4723
Co-fractionation Homo sapiens
2 VCAM1 7412
Affinity Capture-MS Homo sapiens
3 DBT 1629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CLPP 8192
Proximity Label-MS Homo sapiens
5 SLC25A13 10165
Co-fractionation Homo sapiens
6 ZRANB1 54764
Affinity Capture-MS Homo sapiens
7 GPIHBP1  
Affinity Capture-MS Homo sapiens
8 CD1A 909
Affinity Capture-MS Homo sapiens
9 POR 5447
Co-fractionation Homo sapiens
10 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 FAF2 23197
Affinity Capture-MS Homo sapiens
12 HK1 3098
Co-fractionation Homo sapiens
13 CANX 821
Co-fractionation Homo sapiens
14 ATP6V1A 523
Co-fractionation Homo sapiens
15 CD3D 915
Affinity Capture-MS Homo sapiens
16 AMBRA1  
Affinity Capture-MS Homo sapiens
17 PTRF 284119
Co-fractionation Homo sapiens
18 NDUFA9 4704
Co-fractionation Homo sapiens
19 HCST  
Affinity Capture-MS Homo sapiens
20 TPP1 1200
Co-fractionation Homo sapiens
21 NNT 23530
Co-fractionation Homo sapiens
22 LARS2 23395
Affinity Capture-MS Homo sapiens
23 HDAC6 10013
Affinity Capture-MS Homo sapiens
24 PON2 5445
Co-fractionation Homo sapiens
25 PPP6C 5537
Affinity Capture-MS Homo sapiens
26 P4HA1 5033
Co-fractionation Homo sapiens
27 NDUFS1 4719
Co-fractionation Homo sapiens
28 CUL3 8452
Affinity Capture-MS Homo sapiens
29 TRIM33 51592
Affinity Capture-MS Homo sapiens
30 HTT 3064
Co-fractionation Homo sapiens
31 ATP2A2 488
Co-fractionation Homo sapiens
32 LRPPRC 10128
Co-fractionation Homo sapiens
33 LRP1 4035
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TTC5  
Affinity Capture-MS Homo sapiens
35 S100A4 6275
Affinity Capture-MS Homo sapiens
36 NDUFS3 4722
Co-fractionation Homo sapiens
37 UQCRC2 7385
Co-fractionation Homo sapiens
38 VCP 7415
Co-fractionation Homo sapiens
39 CYC1 1537
Co-fractionation Homo sapiens
40 MARS2 92935
Affinity Capture-MS Homo sapiens
41 ASPH 444
Co-fractionation Homo sapiens
42 PPM1K  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 BRD1 23774
Affinity Capture-MS Homo sapiens
44 POLL  
Affinity Capture-MS Homo sapiens
45 UQCRC1 7384
Co-fractionation Homo sapiens
46 ETFDH 2110
Co-fractionation Homo sapiens
47 Hdac6  
Affinity Capture-MS Mus musculus
48 HECTD1 25831
Affinity Capture-MS Homo sapiens
49 POLB 5423
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 BCKDHA 593
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TRAV20  
Affinity Capture-MS Homo sapiens
52 DNAJB5  
Affinity Capture-MS Homo sapiens
53 CEACAM8 1088
Affinity Capture-MS Homo sapiens
54 S100A2 6273
Affinity Capture-MS Homo sapiens
55 COMT 1312
Co-fractionation Homo sapiens
56 TCF25 22980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SP100 6672
Affinity Capture-MS Homo sapiens
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