Gene description for F2R
Gene name coagulation factor II (thrombin) receptor
Gene symbol F2R
Other names/aliases CF2R
HTR
PAR-1
PAR1
TR
Species Homo sapiens
 Database cross references - F2R
ExoCarta ExoCarta_2149
Vesiclepedia VP_2149
Entrez Gene 2149
HGNC 3537
MIM 187930
UniProt P25116  
 F2R identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for F2R
Molecular Function
    G-protein alpha-subunit binding GO:0001965 ISS
    G protein-coupled receptor activity GO:0004930 ISS
    G protein-coupled receptor activity GO:0004930 TAS
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    thrombin-activated receptor activity GO:0015057 IBA
    thrombin-activated receptor activity GO:0015057 IDA
    G-protein beta-subunit binding GO:0031681 ISS
Biological Process
    connective tissue replacement involved in inflammatory response wound healing GO:0002248 IDA
    negative regulation of glomerular filtration GO:0003105 ISS
    inflammatory response GO:0006954 ISS
    G protein-coupled receptor signaling pathway GO:0007186 ISS
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IBA
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IDA
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 ISS
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISS
    establishment of synaptic specificity at neuromuscular junction GO:0007529 ISS
    positive regulation of cell population proliferation GO:0008284 ISS
    negative regulation of cell population proliferation GO:0008285 IDA
    response to wounding GO:0009611 IDA
    anatomical structure morphogenesis GO:0009653 TAS
    platelet activation GO:0030168 IDA
    regulation of blood coagulation GO:0030193 IDA
    positive regulation of blood coagulation GO:0030194 IBA
    positive regulation of blood coagulation GO:0030194 IDA
    positive regulation of cell migration GO:0030335 IMP
    response to lipopolysaccharide GO:0032496 ISS
    regulation of interleukin-1 beta production GO:0032651 ISS
    positive regulation of interleukin-6 production GO:0032755 IDA
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of collagen biosynthetic process GO:0032967 IDA
    positive regulation of Rho protein signal transduction GO:0035025 ISS
    dendritic cell homeostasis GO:0036145 IEA
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEP
    positive regulation of MAPK cascade GO:0043410 IDA
    negative regulation of neuron apoptotic process GO:0043524 ISS
    positive regulation of GTPase activity GO:0043547 IMP
    cell-cell junction maintenance GO:0045217 IMP
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of vasoconstriction GO:0045907 ISS
    positive regulation of smooth muscle contraction GO:0045987 ISS
    positive regulation of receptor signaling pathway via JAK-STAT GO:0046427 NAS
    regulation of synaptic plasticity GO:0048167 ISS
    homeostasis of number of cells within a tissue GO:0048873 ISS
    release of sequestered calcium ion into cytosol GO:0051209 ISS
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISS
    positive regulation of calcium ion transport GO:0051928 ISS
    regulation of sensory perception of pain GO:0051930 ISS
    platelet dense granule organization GO:0060155 IC
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    thrombin-activated receptor signaling pathway GO:0070493 IDA
    trans-synaptic signaling by endocannabinoid, modulating synaptic transmission GO:0099553 IEA
    negative regulation of renin secretion into blood stream GO:1900134 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    Golgi apparatus GO:0005794 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 IDA
    cell surface GO:0009986 IDA
    platelet dense tubular network GO:0031094 IDA
    neuromuscular junction GO:0031594 ISS
    postsynaptic membrane GO:0045211 ISS
 Experiment description of studies that identified F2R in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for F2R
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRPR  
Affinity Capture-MS Homo sapiens
2 SNX1 6642
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 GNA12 2768
Affinity Capture-Western Homo sapiens
4 PDCD6IP 10015
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 CAPN1 823
Co-localization Homo sapiens
6 ATP13A2  
Two-hybrid Homo sapiens
7 CRYL1 51084
Affinity Capture-MS Homo sapiens
8 GNAQ 2776
Affinity Capture-Western Homo sapiens
9 TAB1 10454
Affinity Capture-Western Homo sapiens
10 ARMC6 93436
Affinity Capture-MS Homo sapiens
11 MMP1 4312
Co-localization Homo sapiens
12 CHRD  
Two-hybrid Homo sapiens
13 TAB2  
Affinity Capture-Western Homo sapiens
14 ARRDC3 57561
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
15 GNA13 10672
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 PROCR 10544
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 GNAI2 2771
Affinity Capture-Western Homo sapiens
18 MAPK14 1432
Affinity Capture-Western Homo sapiens
19 AP3D1 8943
Affinity Capture-Western Homo sapiens
20 FLOT2 2319
Affinity Capture-Western Homo sapiens
21 CAV1 857
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 CHMP4A 29082
Affinity Capture-Western Homo sapiens
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