Gene description for Mars
Gene name methionine-tRNA synthetase
Gene symbol Mars
Other names/aliases Metrs
Mtrns
Species Mus musculus
 Database cross references - Mars
ExoCarta ExoCarta_216443
Vesiclepedia VP_216443
Entrez Gene 216443
UniProt Q68FL6  
 Mars identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mars
Molecular Function
    tRNA binding GO:0000049 IEA
    methionine-tRNA ligase activity GO:0004825 IBA
    methionine-tRNA ligase activity GO:0004825 IMP
    methionine-tRNA ligase activity GO:0004825 ISO
    methionine-tRNA ligase activity GO:0004825 ISS
    ATP binding GO:0005524 IEA
Biological Process
    methionyl-tRNA aminoacylation GO:0006431 IBA
    methionyl-tRNA aminoacylation GO:0006431 IDA
    methionyl-tRNA aminoacylation GO:0006431 ISO
    methionyl-tRNA aminoacylation GO:0006431 ISO
    rRNA transcription GO:0009303 ISO
    rRNA transcription GO:0009303 ISO
    cellular response to platelet-derived growth factor stimulus GO:0036120 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    positive regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901838 ISO
Subcellular Localization
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 IBA
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 IDA
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 ISO
 Experiment description of studies that identified Mars in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mars
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Kcna3  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 Hars2  
Co-fractionation Mus musculus
8 Eprs 107508
Co-fractionation Mus musculus
9 Nars2 244141
Co-fractionation Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
11 Kars 85305
Co-fractionation Mus musculus
View the network image/svg+xml



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